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Synthesis of RNA and Protein During Epidermal Cell Differentiation in Man

Synthesis of RNA and Protein During Epidermal Cell Differentiation in Man Abstract Synthesis of RNA and protein taking place during cornification was studied in human epidermis by autoradiography. Cytidine-3H incorporation was observed in almost all nuclei except 17.7% of nuclei scattered randomly and located in the outermost layer. Histidine-3H and arginine-3H concentrated in granular cells, while methionine-3H tended to concentrate in basal cells and phenylalanine-3H had a more general distribution. In the granular layer 10.4% and 10.7% of cells failed to incorporate histidine-3H and arginine-3H, respectively. The results suggest: (1) proteins of different composition are synthesized in different layers of the epidermis; and (2) granular cells stop synthesis of RNA and protein at a finite time before they are completely keratinized. This process occurs independently in granular cells suggesting that cell differentiation is regulated within individual cells. References 1. Amano, M.J.: Synthesis and Behaviour of RNA as Shown by RAG in Mouse Tissue in Vitro: Improved Techniques for the Enzymatic Extraction of Nucleic Acids From Tissue Sections , J Histochem Cytochem 10:204, 1962.Crossref 2. Fitzgerald, P.J., and Vinijchaikul, K.: Nucleic Acid Metabolism of Pancreatic Cells as Revealed by Cytidine-H3 , Lab Invest 8:319, 1959. 3. Fukuyama, K., and Bernstein, I.A.: Site of Synthesis of Ribonucleic Acid in Mammalian Epidermis , J Invest Derm 41:47, 1963.Crossref 4. Leblond, C.P.; Everett, N.B.; and Simmons, B.: Sites of Protein Synthesis as Shown by Radioautography After Administration of S35 Labeled Methionine , Amer J Anat 2:225, 1959. 5. Rothberg, S.; Crounse, R.G.; and Lee, J.L.: Glycine-C14 Incorporation Into the Proteins of Normal Stratum Corneum and the Abnormal Stratum Corneum of Psoriasis , J Invest Derm 37:497, 1961.Crossref 6. Fukuyama, K.; Nakamura, T.; and Bernstein, I.A.: Differentially Localized Incorporation of Amino Acids in Relation to Epidermal Keratinization in the Newborn Rat , Anat Rec 152:525, 1965.Crossref 7. Fukuyama, K., and Epstein, W.L.: Epidermal Keratinization: Localization of Isotopically Labeled Amino Acids , J Invest Derm 47:551, 1966.Crossref 8. Fukuyama, K., and Epstein, W.L.: Protein Synthesis Studied by Autoradiography in the Epidermis of Different Species , Amer J Anat to be published. 9. Rudall, K.M.: The Proteins of the Mammalian Epidermis , Adv Protein Chem 7:253, 1952. 10. Fukuyama, K., and Epstein, W.L.: Ultrastructural autoradiographic Studies of Keratohyalin Granule Formation , J Invest Derm 49:595, 1967.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Synthesis of RNA and Protein During Epidermal Cell Differentiation in Man

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Publisher
American Medical Association
Copyright
Copyright © 1968 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1968.01610130081016
Publisher site
See Article on Publisher Site

Abstract

Abstract Synthesis of RNA and protein taking place during cornification was studied in human epidermis by autoradiography. Cytidine-3H incorporation was observed in almost all nuclei except 17.7% of nuclei scattered randomly and located in the outermost layer. Histidine-3H and arginine-3H concentrated in granular cells, while methionine-3H tended to concentrate in basal cells and phenylalanine-3H had a more general distribution. In the granular layer 10.4% and 10.7% of cells failed to incorporate histidine-3H and arginine-3H, respectively. The results suggest: (1) proteins of different composition are synthesized in different layers of the epidermis; and (2) granular cells stop synthesis of RNA and protein at a finite time before they are completely keratinized. This process occurs independently in granular cells suggesting that cell differentiation is regulated within individual cells. References 1. Amano, M.J.: Synthesis and Behaviour of RNA as Shown by RAG in Mouse Tissue in Vitro: Improved Techniques for the Enzymatic Extraction of Nucleic Acids From Tissue Sections , J Histochem Cytochem 10:204, 1962.Crossref 2. Fitzgerald, P.J., and Vinijchaikul, K.: Nucleic Acid Metabolism of Pancreatic Cells as Revealed by Cytidine-H3 , Lab Invest 8:319, 1959. 3. Fukuyama, K., and Bernstein, I.A.: Site of Synthesis of Ribonucleic Acid in Mammalian Epidermis , J Invest Derm 41:47, 1963.Crossref 4. Leblond, C.P.; Everett, N.B.; and Simmons, B.: Sites of Protein Synthesis as Shown by Radioautography After Administration of S35 Labeled Methionine , Amer J Anat 2:225, 1959. 5. Rothberg, S.; Crounse, R.G.; and Lee, J.L.: Glycine-C14 Incorporation Into the Proteins of Normal Stratum Corneum and the Abnormal Stratum Corneum of Psoriasis , J Invest Derm 37:497, 1961.Crossref 6. Fukuyama, K.; Nakamura, T.; and Bernstein, I.A.: Differentially Localized Incorporation of Amino Acids in Relation to Epidermal Keratinization in the Newborn Rat , Anat Rec 152:525, 1965.Crossref 7. Fukuyama, K., and Epstein, W.L.: Epidermal Keratinization: Localization of Isotopically Labeled Amino Acids , J Invest Derm 47:551, 1966.Crossref 8. Fukuyama, K., and Epstein, W.L.: Protein Synthesis Studied by Autoradiography in the Epidermis of Different Species , Amer J Anat to be published. 9. Rudall, K.M.: The Proteins of the Mammalian Epidermis , Adv Protein Chem 7:253, 1952. 10. Fukuyama, K., and Epstein, W.L.: Ultrastructural autoradiographic Studies of Keratohyalin Granule Formation , J Invest Derm 49:595, 1967.Crossref

Journal

Archives of DermatologyAmerican Medical Association

Published: Jul 1, 1968

References