Abstract Although concentrations of tocopherol in tissues or blood and estimates of dietary intake have been used as indices of vitamin E nutrition, the absence in man of recognized clinical, biochemical, or physiological correlations of vitamin E deficiency has hindered interpretation of the findings.1 Rose and György2 have established that hemolysis of erythrocytes in dilute solutions of hydrogen peroxide can be used as an index of tocopherol deficiency in rats and have reported that some newborn full-term infants show this evidence of deficiency.3 In previous studies, we have reported the presence of positive hemolysis tests in premature and full-term infants fed partially skimmed cows' milk mixtures, in unfed newborn infants, both full-term and premature, and in infants and children with steatorrhea associated with cystic fibrosis of the pancreas or biliary atresia.* It is the purpose of this report to present data correlating plasma tocopherol levels and hemolysis in References 1. Mason, K. E.: The Tocopherols , in the Vitamins , edited by W. H. Sebrell R.S. and Harris, New York, Academic Press, Inc., 1954, Vol. 3. 2. Rose, C. S., and György, P.: Specificity of Hemolytic Reaction in Vitamin E Deficient Erythrocytes , Am. J. Physiol. 168:414, 1952. 3. György, P.; Cogan, G., and Rose, C. S.: Availability of Vitamin E in the Newborn Infant , Proc. Soc. Exper. Biol. & Med. 81:536, 1952. 4. Gordon, H. H., and de Metry, J.: Hemolysis in Hydrogen Peroxide of Erythrocytes of Premature Infants: Effect of Alpha Tocopherol , Proc. Soc. Exper. Biol. & Med. 79:446, 1952. 5. Gordon, H. H.; Nitowsky, H. M., and Cornblath, M.: Studies of Tocopherol Deficiency in Infants and Children: I. Hemolysis of Erythrocytes in Hydrogen Peroxide , A. M. A. Am. J. Dis. Child. 90:669, 1955. 6. MacKenzie, J. B.: Relation Between Serum Tocopherol and Hemolysis in Hydrogen Peroxide of Erythrocytes in Premature Infants , Pediatrics 13:346, 1954. 7. Quaife, M. L.; Scrimshaw, N. S., and Lowry, O. H.: Micromethod for Assay of Total Tocopherols in Blood Serum , J. Biol. Chem. 180:1229, 1949. 8. Moyer, W. T.: Vitamin E Levels in Term and Premature Newborn Infants , Pediatrics 6:893, 1950. 9. Harris, P. L.; Hickman, K. C. D.; Jensen, J. L., and Spies, T. D.: Survey of Blood Plasma Levels of Vitamin A, Carotene, Ascorbic Acid and Tocopherols of Persons in Area of Endemic Malnutrition , Am. J. Pub. Health 36:155, 1946. 10. Chieffi, M., and Kirk, J. E.: Vitamin Studies in Middle-Aged and Old Individuals: IV. Tocopherol Plasma Concentrations , J. Gerontol. 6:17, 1951. 11. Ferguson, M. E.; Bridgeforth, E.; Quaife, M. L.; Martin, M. P.; Cannon, R. O.; McGanity, W. J.; Newhill, J., and Darby, W. J.: The Vanderbilt Cooperative Study of Maternal and Infant Nutrition: VII. Tocopherol in Relation to Pregnancy , J. Nutrition 55:305, 1955. 12. Wright, S. W.; Filer, L. J., Jr., and Mason, K. E.: Vitamin E Blood Levels in Premature and Full Term Infants , Pediatrics 7:386, 1951. 13. Varangot, J.; Chailley, H., and Rieux, N.: Sur la teneur du sang du cordon en vitamine E et sur le passage du tocophérol de la mère au foetus , Compt. rend. Soc. biol. 138: 24, 1944. 14. Straumfjard, J. V., and Quaife, M. L.: Vitamin E Levels in Maternal and Fetal Blood Plasma , Proc. Soc. Exper. Biol. & Med. 61:369, 1946. 15. Filer, L. J.; Wright, S. W.; Manning, M. P., and Mason, K. E.: Absorption of Alpha Tocopherol and Tocopheryl Esters by Premature and Full Term Infants and Children in Health and Disease , Pediatrics 8:328, 1951. 16. Darby, W. J.; Ferguson, M. E.; Furman, R. H.; Lemley, J. M.; Ball, C. T., and Meneely, G. R.: Plasma Tocopherols in Health and Disease , Ann. New York Acad. Sc. 52: 328, 1949. 17. Nitowsky, H. M., and Tildon, J. T.: Studies of Tocopherol Deficiency in Infants and Children: III. Relation of Blood Catalase Activity and Other Factors to Hemolysis of Erythrocytes in Hydrogen Peroxide, Am. J. Clin. Nutrition, to be published. 18. Nitowsky, H. M.; Gordon, H. H., and Tildon, J. T.: Studies of Tocopherol Deficiency in Infants and Children; IV The Effect of Alpha Tocopherol on Creatinuria in Patients with Cystic Fibrosis of the Pancreas and Biliary Atresia, Bull. Johns Hopkins Hosp., to be published. 19. Butler, A. M., and Cushman, M.: Distribution of Ascorbic Acid in the Blood and Its Significance , J. Clin. Invest. 19:459, 1940. 20. Allison, A. C.: Danger of Vitamin K to Newborn, Letters to the Editor , Lancet 1: 669, 1955. 21. Moore, T., and Sharman, I. M.: Danger of Vitamin K Analogues to Newborn, Letters to the Editor , Lancet 1:819, 1955. 22. Laurance, B.: Danger of Vitamin K Analogues to Newborn, Letters to the Editor , Lancet 1:819, 1955. 23. Hollingsworth, J. W.: Lifespan of Fetal Erythrocytes , J. Lab. & Clin. Med. 45:469, 1955. 24. Tappel, A. L.: Studies of the Mechanism of Vitamin E Action: II. Inhibition of Unsaturated Fatty Acid Oxidation Catalyzed by Hematin Compounds , Arch. Biochem. 50:473, 1954.Crossref 25. Collier, H. B., and McRae, S. C.: Lipoxidase Activity of Erythrocytes and Its Inhibition by Antioxidants , Fed. Proc. 14:195, 1955. 26. Woodruff, C. W.: Tocopherol Nutriture in a Patient with Xanthomatous Biliary Cirrhosis, to be published. 27. Woodruff, C. W.: Tocopherol Nutriture in a Patient with Xanthomatous Biliary Cirrhosis, Am. J. Clin. Nutrition, to be published.
A.M.A. Journal of Diseases of Children – American Medical Association
Published: Aug 1, 1956
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