Abstract • One hundred sixty-eight children with upper respiratory infections were screened for β-hemolytic streptococcal pharyngitis. A recently developed throat culture kit was processed in comparison with the conventional blood agar plate using a duplicate swab technique. Kits and plates were examined independently. Three individuals with no previous bacteriologic experience were allowed to interpret results of the culture kit after receiving instructions for approximately 15 minutes on three consecutive days. The interpretations of two of these individuals approached 95% accuracy, and those of the third individual surpassed this rate. The kit appears to be at least equal to the conventional blood agar plate in supporting the growth of β-hemolytic streptococci. Moreover, its selective nature allows the nontechnical office staff to interpret results of the cultures promptly and accurately with a minimum of instruction. (Am J Dis Child 130:171-172, 1976) References 1. Breese BB, Disney FA: The accuracy of diagnosis of β-hemolytic streptococcal infection on clinical grounds . J Pediatr 44:670-673, 1954.Crossref 2. Breese BB: Beta-hemolytic streptococcal infection in children . Pediatr Clin North Am 7:843-867, 1960. 3. Massel BF, Amezcua J, Michael SG: Home cultures as an aid in the diagnosis of streptococcal respiratory infection . N Engl J Med 271:581-585, 1964.Crossref 4. Vincent WF, Gibbons WE, Gaafar HW: Selective media for the isolation of streptococci from clinical specimens . Appl Microbiol 22:942-943, 1971. 5. Maxted WR: The use of bacitracin for identifying group A hemolytic streptococci . J Clin Pathol 6:224-226, 1953.Crossref 6. Katz HP, Clancy RR: Accuracy of a home throat culture program: A study of patient participation in health care . Pediatrics 53:687-691, 1974.
American Journal of Diseases of Children – American Medical Association
Published: Feb 1, 1976