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STERILITY AND CRYOSURGERY

STERILITY AND CRYOSURGERY This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables. Abstract To the Editor: We have read with interest Dr. John G. Bellows' letter on sterility and cryosurgery (Arch Ophthal74:290-291, 1965). Our experiences are consistent with his finding that cryogens are practically free of bacteria. We examined samples of liquid nitrogen by evaporating the samples under sterile conditions and subsequent determining germ count in the container. We found one living bacterium for every 5 to 10 ml of liquid nitrogen. It should be taken into account, however, that even the most dangerous bacteria are not at all impaired at the temperature of the liquid nitrogen. We added lyophilized cultures of Pseudomonas aeruginosa and Staphylococcus aureus thoroughly ground with talc to liquid nitrogen and determined the germ count in the container after the nitrogen had evaporated. The bacteria could be recovered in full number in both cases.We have drawn the conclusion that, in spite of the sterility (in the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

STERILITY AND CRYOSURGERY

Archives of Ophthalmology , Volume 75 (2) – Feb 1, 1966

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Publisher
American Medical Association
Copyright
Copyright © 1966 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1966.00970050304031
Publisher site
See Article on Publisher Site

Abstract

This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables. Abstract To the Editor: We have read with interest Dr. John G. Bellows' letter on sterility and cryosurgery (Arch Ophthal74:290-291, 1965). Our experiences are consistent with his finding that cryogens are practically free of bacteria. We examined samples of liquid nitrogen by evaporating the samples under sterile conditions and subsequent determining germ count in the container. We found one living bacterium for every 5 to 10 ml of liquid nitrogen. It should be taken into account, however, that even the most dangerous bacteria are not at all impaired at the temperature of the liquid nitrogen. We added lyophilized cultures of Pseudomonas aeruginosa and Staphylococcus aureus thoroughly ground with talc to liquid nitrogen and determined the germ count in the container after the nitrogen had evaporated. The bacteria could be recovered in full number in both cases.We have drawn the conclusion that, in spite of the sterility (in the

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Feb 1, 1966

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