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SOCIETY FOR PEDIATRIC RESEARCH

SOCIETY FOR PEDIATRIC RESEARCH Harold E. Harrison, M.D., President S. Sydney Gellis, M.D., Secretary-Treasurer Hill 1954 Annual Buck Pa., 5, 6, and 7, Twenty-Fourth Meeting, Falls, May (Concluded from Page 674) Effect of Pertussis in Human Brain Tissue Cultures. Dr. Harriet M. Hemophilus Felton (by Dr. A. Gaggero and Dr. C. M. Pomerat (by invitation), (by invitation), Texas. invitation), Galveston, Inoculation of cerebellum and of into growing human suspensions Hemophilus pertussis cerebral cortex produces marked cellular in these tissues. Preliminary experiments changes were carried contrast out in a chamber and the results recorded continuous perfusion by phase a in cinematography. As a base 2-hour record was made of fetal cerebellum and cortex line, tissue culture, a of 48-hour of H. was introduced into the suspension growth pertussis prepara- tions, and records were made for a 24-hour At the end of this time the cells showed period. unmistakable There were nuclear the cell outlines were and there damage. changes, shrunken, was little movement. The addition of antiserum almost very marginal specific immediately resulted in reactivation cells had their signs of of cell function. Within several hours the regained former appearance. These observations have been in roller tube preparations using repeated newborn stained at the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American journal of diseases of children American Medical Association

SOCIETY FOR PEDIATRIC RESEARCH

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Publisher
American Medical Association
Copyright
Copyright © 1954 American Medical Association. All Rights Reserved. Applicable FARS/DFARS Restrictions Apply to Government Use.
ISSN
0096-8994
eISSN
1538-3628
DOI
10.1001/archpedi.1954.02050100781013
Publisher site
See Article on Publisher Site

Abstract

Harold E. Harrison, M.D., President S. Sydney Gellis, M.D., Secretary-Treasurer Hill 1954 Annual Buck Pa., 5, 6, and 7, Twenty-Fourth Meeting, Falls, May (Concluded from Page 674) Effect of Pertussis in Human Brain Tissue Cultures. Dr. Harriet M. Hemophilus Felton (by Dr. A. Gaggero and Dr. C. M. Pomerat (by invitation), (by invitation), Texas. invitation), Galveston, Inoculation of cerebellum and of into growing human suspensions Hemophilus pertussis cerebral cortex produces marked cellular in these tissues. Preliminary experiments changes were carried contrast out in a chamber and the results recorded continuous perfusion by phase a in cinematography. As a base 2-hour record was made of fetal cerebellum and cortex line, tissue culture, a of 48-hour of H. was introduced into the suspension growth pertussis prepara- tions, and records were made for a 24-hour At the end of this time the cells showed period. unmistakable There were nuclear the cell outlines were and there damage. changes, shrunken, was little movement. The addition of antiserum almost very marginal specific immediately resulted in reactivation cells had their signs of of cell function. Within several hours the regained former appearance. These observations have been in roller tube preparations using repeated newborn stained at the

Journal

American journal of diseases of children – American Medical Association

Published: Dec 1, 1954

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