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Sheep Erythrocyte Rosette Formation and Phytohemagglutinin-Stimulated Cells: Two Cases of Mycosis Fungoides With Skin Tumors and Lymph Nodes

Sheep Erythrocyte Rosette Formation and Phytohemagglutinin-Stimulated Cells: Two Cases of Mycosis... Abstract Infiltrating cells were harvested by mechanical means from skin tumors and lymph nodes of myocis fungoides. Sheep erythrocyte rosette formation was observed on more than 90% of small lymphocyte-like cells with irregular nuclei but seemed to be hardly detectable on larger cells. The small and large cells that formed erythrocyte rosettes showed similar basic ultrastructures, which suggested that these two types of cells are of the same origin. When cultured with phytohemagglutinin (PHA), 90% of the small cells underwent transformation, and they were found to be lymphoblast-like cells with irregular nuclei by electron microscopy. Only 40% of the large cells were transformed with PHA. (Arch Dermatol 115:847-850, 1979) References 1. Lutzner MA, Jordan HW: The ultrastructure of an abnormal cell in Sézary's syndrome . Blood 31:719-726, 1968. 2. Edelson RC, Kirkpatric CH, Shevach EM, et al: Preferential cutaneous infiltration by neoplastic thymus-derived (T) lymphocytes: Morphologic and functional studies . Ann Intern Med 80:685-692, 1974.Crossref 3. Lutzner MA, Edelson RC, Schein P, et al: Cutaneous T-cell lymphomas: The Sézary syndrome, mycosis fungoides, and related disorders . Ann Intern Med 83:534-552, 1975.Crossref 4. Lay WH, Mendes NF, Bianco C, et al: Binding of sheep red blood cells to a large population of human lymphocytes . Nature 230:531-532, 1971.Crossref 5. Zucker-Franklin D, Melton JW, Quagliata F: Ultrastructural, immunologic, and functional studies on Sézary cells: A neoplastic variant of thymus-derived (T) lymphocytes . Proc Natl Acad Sci USA 71:1877-1881, 1974.Crossref 6. Yata J, Tsukimoto I, Nakagawa T, et al: Application of T and B cell markers in the analysis of immunodeficiency status . Keio J Med 24:335-346, 1975.Crossref 7. Hayakawa H: Transformation of human peripheral blood lymphocytes in vitro: I. Response to phytohemagglutinin . J Allergy 17:188-209, 1968. 8. Yamada M, Yoshinaga H, Higashi K: Electron microscopic observation of dermal infiltration in senile erythroderma . J Clin Electron Microsc 8:499-500, 1975. 9. Flaxman BA, Zelazny G, Van Scott EJ: Nonspecificity of characteristic cells in mycosis fungoides . Arch Dermatol 104:141-147, 1971.Crossref 10. Yoshinaga H, Yamada M: Electron microscopic observation of sheep erythrocyte rosette formation in normal human lymphocytes . Jpn J Dermatol 87:311-314, 1977. 11. Yoshinaga H: Electron microscopic observation of blastoid transformation of lymphocytes stimulated by antigens . Acta Dermatol 68:131-154, 1973. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Sheep Erythrocyte Rosette Formation and Phytohemagglutinin-Stimulated Cells: Two Cases of Mycosis Fungoides With Skin Tumors and Lymph Nodes

Archives of Dermatology , Volume 115 (7) – Jul 1, 1979

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Publisher
American Medical Association
Copyright
Copyright © 1979 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1979.04010070023014
Publisher site
See Article on Publisher Site

Abstract

Abstract Infiltrating cells were harvested by mechanical means from skin tumors and lymph nodes of myocis fungoides. Sheep erythrocyte rosette formation was observed on more than 90% of small lymphocyte-like cells with irregular nuclei but seemed to be hardly detectable on larger cells. The small and large cells that formed erythrocyte rosettes showed similar basic ultrastructures, which suggested that these two types of cells are of the same origin. When cultured with phytohemagglutinin (PHA), 90% of the small cells underwent transformation, and they were found to be lymphoblast-like cells with irregular nuclei by electron microscopy. Only 40% of the large cells were transformed with PHA. (Arch Dermatol 115:847-850, 1979) References 1. Lutzner MA, Jordan HW: The ultrastructure of an abnormal cell in Sézary's syndrome . Blood 31:719-726, 1968. 2. Edelson RC, Kirkpatric CH, Shevach EM, et al: Preferential cutaneous infiltration by neoplastic thymus-derived (T) lymphocytes: Morphologic and functional studies . Ann Intern Med 80:685-692, 1974.Crossref 3. Lutzner MA, Edelson RC, Schein P, et al: Cutaneous T-cell lymphomas: The Sézary syndrome, mycosis fungoides, and related disorders . Ann Intern Med 83:534-552, 1975.Crossref 4. Lay WH, Mendes NF, Bianco C, et al: Binding of sheep red blood cells to a large population of human lymphocytes . Nature 230:531-532, 1971.Crossref 5. Zucker-Franklin D, Melton JW, Quagliata F: Ultrastructural, immunologic, and functional studies on Sézary cells: A neoplastic variant of thymus-derived (T) lymphocytes . Proc Natl Acad Sci USA 71:1877-1881, 1974.Crossref 6. Yata J, Tsukimoto I, Nakagawa T, et al: Application of T and B cell markers in the analysis of immunodeficiency status . Keio J Med 24:335-346, 1975.Crossref 7. Hayakawa H: Transformation of human peripheral blood lymphocytes in vitro: I. Response to phytohemagglutinin . J Allergy 17:188-209, 1968. 8. Yamada M, Yoshinaga H, Higashi K: Electron microscopic observation of dermal infiltration in senile erythroderma . J Clin Electron Microsc 8:499-500, 1975. 9. Flaxman BA, Zelazny G, Van Scott EJ: Nonspecificity of characteristic cells in mycosis fungoides . Arch Dermatol 104:141-147, 1971.Crossref 10. Yoshinaga H, Yamada M: Electron microscopic observation of sheep erythrocyte rosette formation in normal human lymphocytes . Jpn J Dermatol 87:311-314, 1977. 11. Yoshinaga H: Electron microscopic observation of blastoid transformation of lymphocytes stimulated by antigens . Acta Dermatol 68:131-154, 1973.

Journal

Archives of DermatologyAmerican Medical Association

Published: Jul 1, 1979

References