Abstract Abnormal levels of serum enzymes have been observed in a number of diseases.1-4 Attention has been particularly devoted to the study of the serum glutamic oxalacetic transaminase levels in patients with cardiac and hepatic disease.2,3 There has been no systematic study of the serum enzymes in patients with anemia. In a study of lactic dehydrogenase levels of the serum in patients with various diseases4 it was found that the levels of this enzyme were elevated in patients with megaloblastic anemia and in those with sickle-cell disease but not in patients with a number of other types of anemia. The present study constitutes an extension of these observations. The levels of lactic dehydrogenase and glutamic oxalacetic transaminase have been studied in patients with megaloblastic anemia, hemoglobinopathies. other hemolytic anemias, and anemias of other causes. Materials and Methods The serum levels of lactic dehydrogenase (LD) were determined by a References 1. This included a phosphatase-buffered (pH 7.5) mixture of α-ketoglutaric and aspartic acids prepared and standardized by the Sigma Chemical Company, St. Louis, in accordance with the method of Reitman and Frankel.6 2. Sibley, J. A., and Fleisher, G. A.: The Clinical Significance of Serum Aldolase , Proc. Staff Meet. Mayo Clin. 29:591-603, 1954. 3. Mason, J. H., and Wróblewski, F.: Serum Glutamic Oxalacetic Transaminase Activity in Experimental and Disease States: A Review , A. M. A. Arch. Int. Med. 99:245-252, 1957.Crossref 4. Chinsky, M.; Shmagranoff, C. L., and Sherry, S.: Serum Transaminase Activity: Observations in a Large Group of Patients , J. Lab. & Clin. Med. 47:108-118, 1956. 5. Zimmerman, H. J., and Weinstein, H. G.: Lactic Dehydrogenase Activity in Human Serum , J. Lab. & Clin. Med. 48:607-616, 1956. 6. Karmen, A.; Wróblewski, F., and LaDue, J. S.: Transaminase Activity in Human Blood: Appendix; A Note on the Spectrophotometric Assay of Glutamic-Oxaloacetic Transaminase in Human Blood Serum, by A. Karmen , J. Clin. Invest. 34:126-133, 1955. 7. Reitman, S., and Frankel, S.: Personal communication to the authors. 8. Tonhazy, N. E.; White, N. G., and Umbreit, W. W.: Rapid Method for Determination of Glutamic-Aspartic Transaminase in Tissues and Its Application to Radiation Sickness , Arch. Biochem. 28:36-42, 1950. 9. Umbreit, W. W.; Kingsley, G. R.; Schaffert, R. R., and Siplet, H.: Colorimetric Method for Transaminase in Serum or Plasma , J. Lab. & Clin. Med. 49:454-459, 1957. 10. Blanchaer, M. C.; Weiss, P., and Bergsagel, D. E.: The Lactic Dehydrogenase Activity and Pyridine Nucleotide Content of Human Blood Cells in Anemia , Canad. J. M. Sc. 29:108-113, 1951. 11. Blanchaer, M. C., and Baldwin, S. L.: Pyruvate Accumulation in Preserved Blood , J. Appl. Physiol. 6:8-14, 1953. 12. Denstedt, D. F.: The Enzymology of the Erythrocyte , in Blood Cells and Plasma Proteins: Their Statein Nature , edited by J. L. Tullis, New York, Academic Press, Inc., 1953,p. 223. 13. Harris, J. W.; Brewster, H. H.; Ham, T. H., and Castle, W. B.: Studies on the Destrucion off Red Blood Cells: X. The Biophysics and Biology of Sickle-Cell Disease , A. M. A. Arch. Int. Med. 97:145-168, 1956. 14. Singer, K.; Robin, S.; King, E. J., and Jefferson, R. N.: The Life Span of the Sickle Cell and the Pathogenesis of Sickle Cell Anemia , J. Lab. & Clin. Med. 33:975-984, 1948. 15. Ham, T. H.; Weisman, R., Jr., and Hinz, C. F., Jr.: Mechanisms of Destruction of Red Cells in Certain Hemolytic Conditions , A. M. A. Arch. Int. Med. 98:574-592, 1956. 16. Green, T. W.; Conley, C. L., and Berthrong, M.: The Liver in Sickle Cell Anemia , Bull. Johns Hopkins Hosp. 92:99-127, 1953. 17. Bogoch, A.; Casselman, W. G. B., and Margolies, M. P.: Liver Disease in Sickle Cell Anemia , Am. J. Med. 19:583-603, 1955. 18. Singer, K.; King, J. C., and Robin, S.: The Life Span of the Megalocyte and the Hemolytic Syndrome of Pernicious Anemia , J. Lab. & Clin. Med. 33:1068-1076, 1948. 19. Warburg, O., and Christian, W.: Garüngsfermente im Blutserum von Tumor-Ratten , Biochem. Ztschr. 314:399-408, 1943. 20. Hill, B. R., and Levi, C.: Elevation of a Serum Component in Neoplastic Disease , Cancer Res. 14:513-515, 1954. 21. Bierman, H. R.; Hill, B.; Emory, E.; Reinhardt, L., and Samuels, A.: Correlation of Serum Lactic Dehydrogenase Activity with the Clinical Status of Patients with Neoplastic Diseases , Proc. Am. A. Cancer Res. 2:5, 1955. 22. Wróblewski, F., and LaDue, J. S.: Lactic Dehydrogenase Activity in Blood , Proc. Soc. Exper. Biol. & Med. 90:210-213, 1955. 23. West, M.; Heller, P., and Zimmerman, H. J.: Serum Enzymes in Lymphoma and Leukemia, to be published. 24. Hill, B. R.: Some Properties of Serum Lactic Dehydrogenase , Cancer Res. 16:460-467, 1956. 25. Greenstein, J. P.: Biochemistry of Cancer , Ed. 2, New York, Academic Press, Inc., 1954, p. 450. 26. Malnak, A.; Korn, R. J., and Zimmerman, H. J.: Serum Enzymes in Hepatic Disease, to be published.
A.M.A. Archives of Internal Medicine – American Medical Association
Published: Jul 1, 1958