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S060 Serum Protein Profile Analysis in Patients With Papillary Thyroid Carcinoma

S060 Serum Protein Profile Analysis in Patients With Papillary Thyroid Carcinoma Objective: To determine the sensitivity and specificity of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) for papillary thyroid cancer (PTC) detection. Design: SELDI-TOF-MS protein profiles of patients with PTC, benign nodular disease (BND), and healthy controls were analyzed to determine the sensitivity and specificity of SELDI assay for PTC detection. Data analysis was performed using Ciphergen Biomarker Wizards and Biomarker Pattern Software to process the spectral data and classify the disease status of the patients. Subjects: Serum samples were collected prospectively from 7 PTC patients, 8 BND patients, and 7 healthy control volunteers. Intervention: All sample patients underwent thyroidectomy between October 2004 and January 2006. Results: The majority of protein peaks resolved by the SELDI assay were in the range of 1 to 20 kDa. Classification tree analysis based on peak expression distinguished patients with PTC from BND with 85.7% sensitivity and 100% specificity. Serum samples from PTC patients differed most significantly from BND by the underexpression of a protein peak at 11 101 Da. Conclusions: This pilot study demonstrates that proteomic analysis of serum protein profiles distinguishes patients with PTC from patients with BND with a high degree of sensitivity and specificity. Further investigation into the clinical utility of this technology in PTC biomarker detection and surveillance is warranted. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Otolaryngology - Head & Neck Surgery American Medical Association

S060 Serum Protein Profile Analysis in Patients With Papillary Thyroid Carcinoma

S060 Serum Protein Profile Analysis in Patients With Papillary Thyroid Carcinoma

Abstract

Objective: To determine the sensitivity and specificity of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) for papillary thyroid cancer (PTC) detection. Design: SELDI-TOF-MS protein profiles of patients with PTC, benign nodular disease (BND), and healthy controls were analyzed to determine the sensitivity and specificity of SELDI assay for PTC detection. Data analysis was performed using Ciphergen Biomarker Wizards and Biomarker Pattern Software to...
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Publisher
American Medical Association
Copyright
Copyright © 2006 American Medical Association. All Rights Reserved.
ISSN
0886-4470
eISSN
1538-361X
DOI
10.1001/archotol.132.8.851-a
Publisher site
See Article on Publisher Site

Abstract

Objective: To determine the sensitivity and specificity of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) for papillary thyroid cancer (PTC) detection. Design: SELDI-TOF-MS protein profiles of patients with PTC, benign nodular disease (BND), and healthy controls were analyzed to determine the sensitivity and specificity of SELDI assay for PTC detection. Data analysis was performed using Ciphergen Biomarker Wizards and Biomarker Pattern Software to process the spectral data and classify the disease status of the patients. Subjects: Serum samples were collected prospectively from 7 PTC patients, 8 BND patients, and 7 healthy control volunteers. Intervention: All sample patients underwent thyroidectomy between October 2004 and January 2006. Results: The majority of protein peaks resolved by the SELDI assay were in the range of 1 to 20 kDa. Classification tree analysis based on peak expression distinguished patients with PTC from BND with 85.7% sensitivity and 100% specificity. Serum samples from PTC patients differed most significantly from BND by the underexpression of a protein peak at 11 101 Da. Conclusions: This pilot study demonstrates that proteomic analysis of serum protein profiles distinguishes patients with PTC from patients with BND with a high degree of sensitivity and specificity. Further investigation into the clinical utility of this technology in PTC biomarker detection and surveillance is warranted.

Journal

Archives of Otolaryngology - Head & Neck SurgeryAmerican Medical Association

Published: Aug 1, 2006

Keywords: thyroid carcinoma, papillary

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