Abstract CURRENT interest in the effect of viruses on chromosome morphology in man has resulted from the observations made on cultured leukocytes from measles-infected patients1,2 and studies of human fibroblast cultures infected with simian virus 40.3,4 Hampar and Ellison had previously shown that in hamster cell cultures herpes simplex infection produced chromosome changes similar to those reported with x-radiation and chemical mutagens.5 A relationship between the cytologic changes noted with these virus infections of cells and neoplastic changes has been inferred from these studies. It seemed tempting to seek evidence that rubella virus altered chromosome morphology in vivo and in turn to relate such effects to the teratologic disturbances noted in rubella-infected fetuses. We were particularly encouraged by the reports of a mild form of chromosome damage (chromatid breaks) after rubella virus infection of human diploid fibroblasts.6 In the recent rubella epidemic a number of infants have References 1. Nichols, W.W.: Relationship of Viruses, Chromosomes and Carcinogenesis , Hereditas 50:53, 1963.Crossref 2. Harnden, D.G.: Cytogenetic Studies on Patients With Virus Infections and Subjects Vaccinated Against Yellow Fever , Amer J Hum Genet 16:204, 1964. 3. Moorhead, P.S., and Saksela, E.: Non-Random Chromosomal Aberrations in SV-40-transformed Human Cells , J Cell Comp Physiol 62:57, 1963.Crossref 4. Shein, H.M., and Enders, J.F.: Transformation Induced by Simian Virus 40 in Human Renal Cell Cultures: I. Morphology and Growth Characteristics , Proc Nat Acad Sci 48:1164, 1962.Crossref 5. Hampar, B., and Ellison, S.A.: Chromosomal Aberrations Induced by an Animal Virus , Nature 192:145, 1961.Crossref 6. Plotkin, S.A.; Boue, A.; and Boue, J.G.: The in Vitro Growth of Rubella Virus in Human Embryo Cells , Amer J Epid 81:71, 1965. 7. Buckton, K.E., et al: Study of Chromosomal Damage Persisting After X-ray Therapy for Ankylosing Spondylitis , Lancet 2:676, 1962.Crossref 8. Mellman, W.J.: "Human Chromosome Methodology," in Yunis, J. (ed.): Human Peripheral Blood Leucocyte Cultures , New York: Academic Press, Inc., 1965, pp 21-49. 9. Parkman, P.D.; Buescher, E.L.; and Artenstein, M.: Recovery of Rubella Virus From Army Recruits , Proc Soc Exp Biol Med 111:225, 1962.Crossref 10. Plotkin, S.A.: Virological Assistance in the Management of German Measles in Pregnancy , JAMA 190:265, 1964.Crossref 11. Berg, R.B., and Rosenthal, M.S.: Measles Virus in Leucocytes , Proc Soc Exp Biol Med 106:581, 1961.Crossref 12. Berg, R.B.: Multiplication of Echo 9 Virus in Suspensions of Human Leucocytes , Proc Soc Exp Biol Med 108: 742, 1961.Crossref 13. Nahmias, A.J.; Kibrick, S.; and Rosan, R.C.: Viral Leukocyte Interrelationships: I. Multiplication of a DNA Virus—Herpes Simplex—in Human Leukocyte Cultures , J Immun 92:69, 1964.
American Journal of Diseases of Children – American Medical Association
Published: Oct 1, 1965