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Retinal Pigment Epithelial Cells Release an Inhibitor of Neovascularization

Retinal Pigment Epithelial Cells Release an Inhibitor of Neovascularization Abstract • Human retinal pigment epithelial cells in culture were found to release a substance (or substances) that causes the regression of new blood vessels on the chick embryonic yolk sac and inhibits proliferation of fetal bovine aortic endothelial cells in vitro. Neither astrocytes nor fibroblasts, under identical test conditions, released detectable inhibitors of neovascularization or endothelial cell growth. Subconfluent and superconfluent cultures of human retinal pigment epithelial cells released higher levels of inhibitor than confluent cultures. References 1. Folkman J: Tumor angiogenesis: Therapeutic implications . N Engl J Med 1971;285:1182-1186.Crossref 2. Eisenstein R, Sorgente N, Soble LW, et al: The resistance of certain tissues to invasion: Penetrability of explanted tissues by vascular mesenchyme . Am J Pathol 1973;73:765-774. 3. Sorgente N, Kuettner KE, Soble LW, et al: The resistance of certain tissues to invasion: II. Evidence for extractable factors in cartilage which inhibit invasion by vascularized mesenchyme . Lab Invest 1975;32:217-222. 4. Brem H, Folkman J: Inhibition of tumor angiogenesis mediated by cartilage . J Exp Med 1975;141:427-439.Crossref 5. Langer R, Brem H, Falterman K, et al: Isolation of a cartilage factor that inhibits tumor neovascularization . Science 1976;19:70-72.Crossref 6. Brem S, Preis I, Langer R, et al: Inhibition of neovascularization by an extract derived from vitreous . Am J Ophthalmol 1977;84:323-328. 7. Lutty GA, Thompson DC, Gallup JY, et al: Vitreous: An inhibitor of retinal extract-induced neovascularization . Invest Ophthalmol Vis Sci 1983;24:52-56. 8. Williams GA, Eisenstein R, Schumacher B, et al: Inhibitor of vascular endothelial cell growth in the lens . Am J Ophthalmol 1984;97:366-371. 9. Lee A, Langer R: Shark cartilage contains inhibitors of tumor angiogenesis . Science 1983; 221:1185-1187.Crossref 10. Beetham WP, Aiello LM, Balodimos MC, et al: Ruby-laser photocoagulation of early diabetic neovascular retinopathy: Preliminary report of a long-term controlled study . Trans Am Ophthalmol Soc 1969;67:39-67. 11. Doft BH, Blankenship G: Retinopathy risk factor regression after laser panretinal photocoagulation for proliferative diabetic retinopathy . Ophthalmology 1984;91:1453-1457.Crossref 12. Diabetic Retinopathy Study Group: Photocoagulation treatment of proliferative diabetic retinopathy: The second report of diabetic retinopathy findings . Ophthalmology 1978;85:82-106.Crossref 13. Weiter JJ, Zuckerman R: The influence of the photoreceptor-RPE complex on the inner retina: An explanation for the beneficial effects of photocoagulation . Ophthalmology 1980;87: 1133-1139.Crossref 14. Foulds WS: The role of photocoagulation in the treatment of retinal disease . Trans Ophthalmol Soc NZ 1980;32:82-90. 15. Stefansson E, Landers MB, Wolbarsht ML: Oxygenation and vasodilation in relation to diabetic and other proliferative retinopathies . Ophthalmic Surg 1983;14:209-226. 16. Vidaurri-Leal JS, Hohman R, Glaser BM: Effect of vitreous on retinal pigment epithelial cell morphology: A new approach to the study of proliferative vitreoretinopathy . Arch Ophthalmol 1984;102:1220-1223.Crossref 17. McCarthy KD, deVellis J: Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue . J Cell Biol 1980; 85:890-902.Crossref 18. Raff MC, Fields KL, Hakamori SI, et al: Cell-type-specific markers for distinguishing and studying neurons and the major classes of glial cells in culture . Brain Res 1979;174:283-308.Crossref 19. Parks DR, Bryan VM, Oi VT, et al: Antigen-specific identification and cloning of hybridomas with a fluorescence activated cell-sorter . Proc Natl Acad Sci USA 1979;76:1962-1966.Crossref 20. Glaser BM, D'Amore PA, Michels RG, et al: Demonstration of vasoproliferative activity from mammalian retina . J Cell Biol 1980;84:298-304.Crossref 21. Taylor S, Folkman J: Protamine is an inhibitor of angiogenesis . Nature 1982;297:307-312.Crossref 22. Lowry OH, Rosebrough NJ, Farr AL, et al: Protein measurement with the Folin phenol reagent . J Biol Chem 1951;193:265-275. 23. Eckel RH, Fujimoto WY: Quantification of cell death in human fibroblasts by measuring the loss of [14C] thymidine from prelabeled cell monolayers . Biochemistry 1981;114:118-124. 24. Ausprunk DH, Falterman K, Folkman J: The sequence of events in the regression of corneal capillaries . Lab Invest 1978;38:284-294. 25. Wallow IHL, Tso MOM, Fine BS: Retinal repair after experimental xenon arc photocoagulation: I. A comparison between rhesus monkey and rabbit . Am J Ophthalmol 1973;75:32-52. 26. Wallow IHL, Tso MOM: Repair after xenon arc photocoagulation: III. An electron microscopic study of the evolution of retinal lesions in rhesus monkeys . Am J Ophthalmol 1973;75:957-972. 27. Wallow IHL, Davis MD: Clinicopathologic correlation of xenon arc and argon laser photocoagulation procedure in human diabetic eyes . Arch Ophthalmol 1979;97:2308-2315.Crossref 28. Eisenstein R, Kuettner KE, Neopolitan C, et al: The resistance of certain tissues to invasion: III. Cartilage extracts inhibit the growth of fibroblasts and endothelial cells in culture . Am J Pathol 1975;81:337-348. 29. Goren SB, Eisenstein R, Chromokos E: The inhibition of corneal vascularization in rabbits . Am J Ophthalmol 1977;84:305-309. 30. Rifkin DB, Gross JL, Moscatelli D, et al: Proteases and angiogenesis: Production of plasminogen activator and collagenase by endothelial cells , in Nossel HL, Vogel JH (eds): Pathobiology of the Endothelial Cell . New York, Academic Press Inc, 1982, pp 191-197. 31. Glaser BM, Kalebic T, Garbisa S, et al, in Nugent J, O'Connor M (eds): Development of the Vascular System: Ciba Foundation Symposium 100. London, Pitman Medical Books Ltd, 1983, pp 150-162. 32. Kalebic T, Garbisa S, Glaser BM, et al: Basement membrane collagen: Degradation of migrating endothelial cells . Science 1983;221:281-283.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Retinal Pigment Epithelial Cells Release an Inhibitor of Neovascularization

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Publisher
American Medical Association
Copyright
Copyright © 1985 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1985.01050120104029
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See Article on Publisher Site

Abstract

Abstract • Human retinal pigment epithelial cells in culture were found to release a substance (or substances) that causes the regression of new blood vessels on the chick embryonic yolk sac and inhibits proliferation of fetal bovine aortic endothelial cells in vitro. Neither astrocytes nor fibroblasts, under identical test conditions, released detectable inhibitors of neovascularization or endothelial cell growth. Subconfluent and superconfluent cultures of human retinal pigment epithelial cells released higher levels of inhibitor than confluent cultures. References 1. Folkman J: Tumor angiogenesis: Therapeutic implications . N Engl J Med 1971;285:1182-1186.Crossref 2. Eisenstein R, Sorgente N, Soble LW, et al: The resistance of certain tissues to invasion: Penetrability of explanted tissues by vascular mesenchyme . Am J Pathol 1973;73:765-774. 3. Sorgente N, Kuettner KE, Soble LW, et al: The resistance of certain tissues to invasion: II. Evidence for extractable factors in cartilage which inhibit invasion by vascularized mesenchyme . Lab Invest 1975;32:217-222. 4. Brem H, Folkman J: Inhibition of tumor angiogenesis mediated by cartilage . J Exp Med 1975;141:427-439.Crossref 5. Langer R, Brem H, Falterman K, et al: Isolation of a cartilage factor that inhibits tumor neovascularization . Science 1976;19:70-72.Crossref 6. Brem S, Preis I, Langer R, et al: Inhibition of neovascularization by an extract derived from vitreous . Am J Ophthalmol 1977;84:323-328. 7. Lutty GA, Thompson DC, Gallup JY, et al: Vitreous: An inhibitor of retinal extract-induced neovascularization . Invest Ophthalmol Vis Sci 1983;24:52-56. 8. Williams GA, Eisenstein R, Schumacher B, et al: Inhibitor of vascular endothelial cell growth in the lens . Am J Ophthalmol 1984;97:366-371. 9. Lee A, Langer R: Shark cartilage contains inhibitors of tumor angiogenesis . Science 1983; 221:1185-1187.Crossref 10. Beetham WP, Aiello LM, Balodimos MC, et al: Ruby-laser photocoagulation of early diabetic neovascular retinopathy: Preliminary report of a long-term controlled study . Trans Am Ophthalmol Soc 1969;67:39-67. 11. Doft BH, Blankenship G: Retinopathy risk factor regression after laser panretinal photocoagulation for proliferative diabetic retinopathy . Ophthalmology 1984;91:1453-1457.Crossref 12. Diabetic Retinopathy Study Group: Photocoagulation treatment of proliferative diabetic retinopathy: The second report of diabetic retinopathy findings . Ophthalmology 1978;85:82-106.Crossref 13. Weiter JJ, Zuckerman R: The influence of the photoreceptor-RPE complex on the inner retina: An explanation for the beneficial effects of photocoagulation . Ophthalmology 1980;87: 1133-1139.Crossref 14. Foulds WS: The role of photocoagulation in the treatment of retinal disease . Trans Ophthalmol Soc NZ 1980;32:82-90. 15. Stefansson E, Landers MB, Wolbarsht ML: Oxygenation and vasodilation in relation to diabetic and other proliferative retinopathies . Ophthalmic Surg 1983;14:209-226. 16. Vidaurri-Leal JS, Hohman R, Glaser BM: Effect of vitreous on retinal pigment epithelial cell morphology: A new approach to the study of proliferative vitreoretinopathy . Arch Ophthalmol 1984;102:1220-1223.Crossref 17. McCarthy KD, deVellis J: Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue . J Cell Biol 1980; 85:890-902.Crossref 18. Raff MC, Fields KL, Hakamori SI, et al: Cell-type-specific markers for distinguishing and studying neurons and the major classes of glial cells in culture . Brain Res 1979;174:283-308.Crossref 19. Parks DR, Bryan VM, Oi VT, et al: Antigen-specific identification and cloning of hybridomas with a fluorescence activated cell-sorter . Proc Natl Acad Sci USA 1979;76:1962-1966.Crossref 20. Glaser BM, D'Amore PA, Michels RG, et al: Demonstration of vasoproliferative activity from mammalian retina . J Cell Biol 1980;84:298-304.Crossref 21. Taylor S, Folkman J: Protamine is an inhibitor of angiogenesis . Nature 1982;297:307-312.Crossref 22. Lowry OH, Rosebrough NJ, Farr AL, et al: Protein measurement with the Folin phenol reagent . J Biol Chem 1951;193:265-275. 23. Eckel RH, Fujimoto WY: Quantification of cell death in human fibroblasts by measuring the loss of [14C] thymidine from prelabeled cell monolayers . Biochemistry 1981;114:118-124. 24. Ausprunk DH, Falterman K, Folkman J: The sequence of events in the regression of corneal capillaries . Lab Invest 1978;38:284-294. 25. Wallow IHL, Tso MOM, Fine BS: Retinal repair after experimental xenon arc photocoagulation: I. A comparison between rhesus monkey and rabbit . Am J Ophthalmol 1973;75:32-52. 26. Wallow IHL, Tso MOM: Repair after xenon arc photocoagulation: III. An electron microscopic study of the evolution of retinal lesions in rhesus monkeys . Am J Ophthalmol 1973;75:957-972. 27. Wallow IHL, Davis MD: Clinicopathologic correlation of xenon arc and argon laser photocoagulation procedure in human diabetic eyes . Arch Ophthalmol 1979;97:2308-2315.Crossref 28. Eisenstein R, Kuettner KE, Neopolitan C, et al: The resistance of certain tissues to invasion: III. Cartilage extracts inhibit the growth of fibroblasts and endothelial cells in culture . Am J Pathol 1975;81:337-348. 29. Goren SB, Eisenstein R, Chromokos E: The inhibition of corneal vascularization in rabbits . Am J Ophthalmol 1977;84:305-309. 30. Rifkin DB, Gross JL, Moscatelli D, et al: Proteases and angiogenesis: Production of plasminogen activator and collagenase by endothelial cells , in Nossel HL, Vogel JH (eds): Pathobiology of the Endothelial Cell . New York, Academic Press Inc, 1982, pp 191-197. 31. Glaser BM, Kalebic T, Garbisa S, et al, in Nugent J, O'Connor M (eds): Development of the Vascular System: Ciba Foundation Symposium 100. London, Pitman Medical Books Ltd, 1983, pp 150-162. 32. Kalebic T, Garbisa S, Glaser BM, et al: Basement membrane collagen: Degradation of migrating endothelial cells . Science 1983;221:281-283.Crossref

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Dec 1, 1985

References