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Retinal Pigment Epithelial Cells in Culture Produce Retinal Vascular Mitogens

Retinal Pigment Epithelial Cells in Culture Produce Retinal Vascular Mitogens Abstract • Conditioned medium from human retinal pigment epithelial cells in culture significantly stimulated the proliferation of subcultured bovine retinal capillary endothelial cells, pericytes, and freshly isolated retinal microvessels. The vascular mitogenic activity enhanced that of retinal crude extract. No evidence emerged of an inhibitor of microvascular cellular proliferation produced by retinal pigment epithelial cells. References 1. Marshall J, Clover G, Rothery S: Some new findings on retinal irradiation by krypton and argon lasers . Doc Ophthalmol 1984;36:21-37. 2. Bryan J, Campochiaro PA: A retinal pigment epithelial cell-derived growth factor(s) . Arch Ophthalmol 1986;104:422-425.Crossref 3. D'Amore P, Klagsburn M: Endothelial cell mitogens derived from retina and hypothalamus: Biochemical and biological similarities . J Cell Biol 1984;99:1545-1549.Crossref 4. Baird A, Esch F, Gospodarowicz D, et al: Retina- and eye-derived endothelial cell growth factor: Partial molecular characterization and identity with acidic and basic growth factors . Biochemistry 1985;24:7855-7860.Crossref 5. Glaser BM, Campochiaro PA, Davis JL, et al: Retinal pigment epithelial cells release an inhibitor of neovascularization . Arch Ophthalmol 1985;103:1870-1875.Crossref 6. Glaser BM, Campochiaro PA, Davis JL, et al: Retinal pigment epithelial cells release inhibitors of neovascularization . Ophthalmology 1987;94:780-784.Crossref 7. Patz A: A new look at the retinal pigment epithelium and its neighbors, editorial . Arch Ophthalmol 1985;103:1794-1795. 8. Boulton ME, Wong HC, Clark P, et al: A mitogenic factor or factors produced by cultured human retinal pigment epithelial cells , in Ryan SJ, Glaser BM, Murphy RP (ed): Ocular Circulation and Neovascularization . Dordrecht, the Netherlands, Martinus Nijhoff Publishers/Dr W Junk Publishers, 1987, pp 529-534. 9. Wong HC, Boulton ME, Clark P, et al: Retinal pigment epithelial cells produce mitogenic factors for retinal microvascular cells in culture: A preliminary report . Eye 1987;1:754-756. 10. Connor T, Glaser BM: RPE cells can simultaneously release inhibitors and stimulators of endothelial cell proliferation . Invest Ophthalmol Vis Sci 1987;28( (suppl) ):203. 11. Boulton ME, Marshall J, Mellerio J: Retinitis pigmentosa: A preliminary report on tissue culture studies of retinal pigment epithelial cells from eight affected human eyes . Exp Eye Res 1983;37:307-313. 12. Wong HC, Boulton ME, Marshall J, et al: Growth of retinal capillary endothelia using pericyte conditioned medium . Invest Ophthalmol Vis Sci 1987;28:1767-1775. 13. Pledger WJ, Stiles CD, Antoniades HN, et al: Induction of DNA synthesis in BALB/c 3T3 cells by serum components: Reevaluation of the commitment process . Proc Natl Acad Sci USA 1977;74:4481-4485.Crossref 14. Gitlin JD, D'Amore PA: Culture of retinal capillary cells using selective growth media . Microvas Res 1983;26:74-80.Crossref 15. Thornton SC, Mueller SN, Levine EM: Human endothelial cells: Use of heparin in cloning and long-term serial cultivation . Science 1983;222:623-625.Crossref 16. Winer JB: Statistical Principles in Experimental Design . New York, McGraw-Hill International Book Co, 1971. 17. Wallow IHC, Geldner PS: Endothelial fenestrae in proliferative diabetic retinopathy . Invest Ophthalmol Vis Sci 1980;19:1176-1183. 18. Taniguchi Y: Ultrastructure of newly formed blood vessels in diabetic retinopathy . Jpn J Ophthalmol 1976;20:19-31. 19. Morse LS, Sidikaro Y, Terrell J: Retinal pigment epithelium promotes proliferation of choroidal microvessels in vitro . Invest Ophthalmol Vis Sci 1986;27( (suppl) ):327. 20. Orlidge A, D'Amore PA: Inhibition of capillary endothelial cell growth by pericytes and smooth muscle cells . J Cell Biol 1987;105:1455-1462.Crossref 21. Schweigerer L, Malerstein B, Neufield G, et al: Basic fibroblast growth factor is synthesized in cultured retinal pigment epithelial cells . Biochem Biophys Res Commun 1987;143:934-942.Crossref 22. Zetter B: The endothelial cell of large and small blood vessels . Diabetes 1981;30( (suppl 2) ):24-28.Crossref 23. Glaser BM, Kalebic T, Garbisa S, et al: Degradation of basement membrane components by vascular endothelial cells: Role in neovascularization , in Ryan SS, Dawson AK, Little HL (eds): Retinal Diseases . New York, Grune & Stratton, 1985, pp 21-24. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Retinal Pigment Epithelial Cells in Culture Produce Retinal Vascular Mitogens

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References (26)

Publisher
American Medical Association
Copyright
Copyright © 1988 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1988.01060140603031
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Abstract

Abstract • Conditioned medium from human retinal pigment epithelial cells in culture significantly stimulated the proliferation of subcultured bovine retinal capillary endothelial cells, pericytes, and freshly isolated retinal microvessels. The vascular mitogenic activity enhanced that of retinal crude extract. No evidence emerged of an inhibitor of microvascular cellular proliferation produced by retinal pigment epithelial cells. References 1. Marshall J, Clover G, Rothery S: Some new findings on retinal irradiation by krypton and argon lasers . Doc Ophthalmol 1984;36:21-37. 2. Bryan J, Campochiaro PA: A retinal pigment epithelial cell-derived growth factor(s) . Arch Ophthalmol 1986;104:422-425.Crossref 3. D'Amore P, Klagsburn M: Endothelial cell mitogens derived from retina and hypothalamus: Biochemical and biological similarities . J Cell Biol 1984;99:1545-1549.Crossref 4. Baird A, Esch F, Gospodarowicz D, et al: Retina- and eye-derived endothelial cell growth factor: Partial molecular characterization and identity with acidic and basic growth factors . Biochemistry 1985;24:7855-7860.Crossref 5. Glaser BM, Campochiaro PA, Davis JL, et al: Retinal pigment epithelial cells release an inhibitor of neovascularization . Arch Ophthalmol 1985;103:1870-1875.Crossref 6. Glaser BM, Campochiaro PA, Davis JL, et al: Retinal pigment epithelial cells release inhibitors of neovascularization . Ophthalmology 1987;94:780-784.Crossref 7. Patz A: A new look at the retinal pigment epithelium and its neighbors, editorial . Arch Ophthalmol 1985;103:1794-1795. 8. Boulton ME, Wong HC, Clark P, et al: A mitogenic factor or factors produced by cultured human retinal pigment epithelial cells , in Ryan SJ, Glaser BM, Murphy RP (ed): Ocular Circulation and Neovascularization . Dordrecht, the Netherlands, Martinus Nijhoff Publishers/Dr W Junk Publishers, 1987, pp 529-534. 9. Wong HC, Boulton ME, Clark P, et al: Retinal pigment epithelial cells produce mitogenic factors for retinal microvascular cells in culture: A preliminary report . Eye 1987;1:754-756. 10. Connor T, Glaser BM: RPE cells can simultaneously release inhibitors and stimulators of endothelial cell proliferation . Invest Ophthalmol Vis Sci 1987;28( (suppl) ):203. 11. Boulton ME, Marshall J, Mellerio J: Retinitis pigmentosa: A preliminary report on tissue culture studies of retinal pigment epithelial cells from eight affected human eyes . Exp Eye Res 1983;37:307-313. 12. Wong HC, Boulton ME, Marshall J, et al: Growth of retinal capillary endothelia using pericyte conditioned medium . Invest Ophthalmol Vis Sci 1987;28:1767-1775. 13. Pledger WJ, Stiles CD, Antoniades HN, et al: Induction of DNA synthesis in BALB/c 3T3 cells by serum components: Reevaluation of the commitment process . Proc Natl Acad Sci USA 1977;74:4481-4485.Crossref 14. Gitlin JD, D'Amore PA: Culture of retinal capillary cells using selective growth media . Microvas Res 1983;26:74-80.Crossref 15. Thornton SC, Mueller SN, Levine EM: Human endothelial cells: Use of heparin in cloning and long-term serial cultivation . Science 1983;222:623-625.Crossref 16. Winer JB: Statistical Principles in Experimental Design . New York, McGraw-Hill International Book Co, 1971. 17. Wallow IHC, Geldner PS: Endothelial fenestrae in proliferative diabetic retinopathy . Invest Ophthalmol Vis Sci 1980;19:1176-1183. 18. Taniguchi Y: Ultrastructure of newly formed blood vessels in diabetic retinopathy . Jpn J Ophthalmol 1976;20:19-31. 19. Morse LS, Sidikaro Y, Terrell J: Retinal pigment epithelium promotes proliferation of choroidal microvessels in vitro . Invest Ophthalmol Vis Sci 1986;27( (suppl) ):327. 20. Orlidge A, D'Amore PA: Inhibition of capillary endothelial cell growth by pericytes and smooth muscle cells . J Cell Biol 1987;105:1455-1462.Crossref 21. Schweigerer L, Malerstein B, Neufield G, et al: Basic fibroblast growth factor is synthesized in cultured retinal pigment epithelial cells . Biochem Biophys Res Commun 1987;143:934-942.Crossref 22. Zetter B: The endothelial cell of large and small blood vessels . Diabetes 1981;30( (suppl 2) ):24-28.Crossref 23. Glaser BM, Kalebic T, Garbisa S, et al: Degradation of basement membrane components by vascular endothelial cells: Role in neovascularization , in Ryan SS, Dawson AK, Little HL (eds): Retinal Diseases . New York, Grune & Stratton, 1985, pp 21-24.

Journal

Archives of Ophthalmology – American Medical Association

Published: Oct 1, 1988

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