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Removal of Erythrocytes from the Circulation

Removal of Erythrocytes from the Circulation Abstract Labeling erythrocytes with radioactive chromium (Cr51) has made possible study of the fate of normal and diseased erythrocytes.1 The ability of the spleen to sequester red blood cells has been demonstrated in numerous pathological states, the most prominent of which are hereditary spherocytosis, certain autoimmune hemolytic anemias, cirrhosis of the liver, and Gaucher's disease. In an effort to determine why some red cells are destroyed in the spleen while others are not, Harris, McAlister, and Prankerd studied the sites of destruction of red cells with both induced and naturally occurring abnormalities.2 Spherocytes produced by heating red cells for 15 minutes at 50 C and red cells whose surface lipids had been reduced by alumina were selectively sequestered in the spleen after injection. Red cells in which metabolism had been inhibited by incubation in sodium arsenate were destroyed in areas other than the spleen. An extensive series of References 1. We are indebted to Dr. W. H. Zinkham for the determination of GSH and G-6-PD. 2. In contrast to the studies in human subjects, the dog's erythrocytes were relatively more resistant to heat, requiring 70-90 minutes heating at 50 C to achieve splenic sequestration. 3. Jandl, J. H.; Greenberg, M. S.; Yonemoto, R. H., and Castle, W. B.: Clinical Determination of the Sites of Red Cell Sequestration in Hemolytic Anemias , J. Clin. Invest. 35:842, 1956.Crossref 4. Harris, I. M.; McAlister, J. M., and Prankerd, T. A. J.: The Relationship of Abnormal Red Cells to the Normal Spleen , Clin. Sci. 16:223, 1957. 5. Jandl, J. H., and Simmons, R. L.: The Agglutination and Sensitization of Red Cells by Metallic Cations: Interactions Between Multivalent Metals and the Red Cell Membrane , Brit. J. Haemat. 3:19, 1957.Crossref 6. Jandl, J. H.; Jones, A. R., and Castle, W. B.: The Destruction of Red Cells by Antibodies in Man: I. Observations on the Sequestration and Lysis of Red Cells Altered by Immune Mechanisms , J. Clin. Invest. 36:1428, 1957.Crossref 7. Jacob, H. S., and Jandl, J. H.: Effect of Membrane Sulfhydryl Deficiency on the Shape and Survival of Red Cells , Clin. Res. 9:162, 1961. 8. Jandl, J. H., and Kaplan, M. E.: The Destruction of Red Cells by Antibodies in Man: III. Quantitative Factors Influencing the Patterns of Hemolysis in Vivo , J. Clin. Invest. 39:1145, 1960.Crossref 9. Beutler, E.: The Glutathione Instability of Drug-Sensitive Red Cells , J. Lab. Clin. Med. 49:84, 1957. 10. Zinkham, W. H., and Lenhard, R. E.: Metabolic Abnormalities of Erythrocytes from Patients with Congenital Nonspherocytic Hemolytic Anemia , J. Pediat. 55:319, 1959.Crossref 11. Marmount, A. M., and Fusco, F. A.: Perspectives on the Role of the RES in the Steroid-Induced Remissions of Autoimmune Blood Disorders, with Special Regard to Acquired Hemolytic Anemias , in Reticuloendothelial Structure and Function , edited by John H. Heller, New York, The Ronald Press Company, 1960. 12. Cutbush, M., and Mollison, P. L.: Relation Between Characteristics of Blood-Group Antibodies in Vitro and Associated Patterns of Red-Cell Destruction in Vivo , Brit. J. Haemat. 4:115, 1958.Crossref 13. Hughes Jones, N. C., and Szur, L.: Determination of the Sites of Red-Cell Destruction Using Cr51-Labeled Cells , Brit. J. Haemat. 3:320, 1957.Crossref 14. Prankerd, T. A. J.: The Aging of Red Cells , J. Physiol. (Lond.) 143:325, 1958. 15. Jandl, J. H.: The Anemia of Liver Disease: Observations on Its Mechanism , J. Clin. Invest. 34:390, 1955.Crossref 16. Crosby, W. H.: Normal Functions of the Spleen Relative to Red Blood Cells: A Review , Blood 14:399, 1959. 17. Green, T. W.; Conley, C. L., and Berthrong, M.: The Liver in Sickle Cell Anemia , Bull. Johns Hopkins Hosp. 92:99, 1953. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Internal Medicine American Medical Association

Removal of Erythrocytes from the Circulation

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References (17)

Publisher
American Medical Association
Copyright
Copyright © 1962 American Medical Association. All Rights Reserved.
ISSN
0003-9926
eISSN
1538-3679
DOI
10.1001/archinte.1962.03620190092014
Publisher site
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Abstract

Abstract Labeling erythrocytes with radioactive chromium (Cr51) has made possible study of the fate of normal and diseased erythrocytes.1 The ability of the spleen to sequester red blood cells has been demonstrated in numerous pathological states, the most prominent of which are hereditary spherocytosis, certain autoimmune hemolytic anemias, cirrhosis of the liver, and Gaucher's disease. In an effort to determine why some red cells are destroyed in the spleen while others are not, Harris, McAlister, and Prankerd studied the sites of destruction of red cells with both induced and naturally occurring abnormalities.2 Spherocytes produced by heating red cells for 15 minutes at 50 C and red cells whose surface lipids had been reduced by alumina were selectively sequestered in the spleen after injection. Red cells in which metabolism had been inhibited by incubation in sodium arsenate were destroyed in areas other than the spleen. An extensive series of References 1. We are indebted to Dr. W. H. Zinkham for the determination of GSH and G-6-PD. 2. In contrast to the studies in human subjects, the dog's erythrocytes were relatively more resistant to heat, requiring 70-90 minutes heating at 50 C to achieve splenic sequestration. 3. Jandl, J. H.; Greenberg, M. S.; Yonemoto, R. H., and Castle, W. B.: Clinical Determination of the Sites of Red Cell Sequestration in Hemolytic Anemias , J. Clin. Invest. 35:842, 1956.Crossref 4. Harris, I. M.; McAlister, J. M., and Prankerd, T. A. J.: The Relationship of Abnormal Red Cells to the Normal Spleen , Clin. Sci. 16:223, 1957. 5. Jandl, J. H., and Simmons, R. L.: The Agglutination and Sensitization of Red Cells by Metallic Cations: Interactions Between Multivalent Metals and the Red Cell Membrane , Brit. J. Haemat. 3:19, 1957.Crossref 6. Jandl, J. H.; Jones, A. R., and Castle, W. B.: The Destruction of Red Cells by Antibodies in Man: I. Observations on the Sequestration and Lysis of Red Cells Altered by Immune Mechanisms , J. Clin. Invest. 36:1428, 1957.Crossref 7. Jacob, H. S., and Jandl, J. H.: Effect of Membrane Sulfhydryl Deficiency on the Shape and Survival of Red Cells , Clin. Res. 9:162, 1961. 8. Jandl, J. H., and Kaplan, M. E.: The Destruction of Red Cells by Antibodies in Man: III. Quantitative Factors Influencing the Patterns of Hemolysis in Vivo , J. Clin. Invest. 39:1145, 1960.Crossref 9. Beutler, E.: The Glutathione Instability of Drug-Sensitive Red Cells , J. Lab. Clin. Med. 49:84, 1957. 10. Zinkham, W. H., and Lenhard, R. E.: Metabolic Abnormalities of Erythrocytes from Patients with Congenital Nonspherocytic Hemolytic Anemia , J. Pediat. 55:319, 1959.Crossref 11. Marmount, A. M., and Fusco, F. A.: Perspectives on the Role of the RES in the Steroid-Induced Remissions of Autoimmune Blood Disorders, with Special Regard to Acquired Hemolytic Anemias , in Reticuloendothelial Structure and Function , edited by John H. Heller, New York, The Ronald Press Company, 1960. 12. Cutbush, M., and Mollison, P. L.: Relation Between Characteristics of Blood-Group Antibodies in Vitro and Associated Patterns of Red-Cell Destruction in Vivo , Brit. J. Haemat. 4:115, 1958.Crossref 13. Hughes Jones, N. C., and Szur, L.: Determination of the Sites of Red-Cell Destruction Using Cr51-Labeled Cells , Brit. J. Haemat. 3:320, 1957.Crossref 14. Prankerd, T. A. J.: The Aging of Red Cells , J. Physiol. (Lond.) 143:325, 1958. 15. Jandl, J. H.: The Anemia of Liver Disease: Observations on Its Mechanism , J. Clin. Invest. 34:390, 1955.Crossref 16. Crosby, W. H.: Normal Functions of the Spleen Relative to Red Blood Cells: A Review , Blood 14:399, 1959. 17. Green, T. W.; Conley, C. L., and Berthrong, M.: The Liver in Sickle Cell Anemia , Bull. Johns Hopkins Hosp. 92:99, 1953.

Journal

Archives of Internal Medicine – American Medical Association

Published: Jul 1, 1962

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