Abstract THERE has been in use in this laboratory for several months a procedure for staining and mounting skin scrapings and hairs which is exceedingly simple and rapid, yields permanent preparations and produces slides of a quality equal to or better than those obtained by more complicated methods.1 The use of polyvinyl alcohol-lactophenol-cotton blue2 permits more rapid preparation of "permanent" mounts than does the new method, but fungus structures stained with the PVA medium lose their color quickly and cotton blue so applied penetrates clinical materials poorly. Tinea versicolor scales, prepared by the method about to be detailed, have remained in excellent condition for seven months (fig. 1), as have skin scrapings containing Trichophyton hyphae (fig. 2). The general procedure will be described first, followed by suggested modifications for different categories of clinical specimens. A. MATERIALS REQUIRED The following materials are required for the procedure: 1. Solution of methylene blue hydrochloride, prepared References 1. Muskatblit, E.: Staining of Fungi in Scales and Hairs: Method of Staining with Polychrome Methylene Blue , Arch. Dermat. & Syph. 59:236-242 ( (Feb.) ) 1949. 2. Huber, W. H., and Caplin, S. M.: Simple Plastic Mount for Permanent Preservation of Fungi and Small Arthropods , Arch. Dermat. & Syph. 56:763-765 ( (Dec.) ) 1947.
A.M.A. Archives of Dermatology & Syphilology – American Medical Association
Published: Mar 1, 1951