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Rapid Parlodion Embedding of Temporal Bones

Rapid Parlodion Embedding of Temporal Bones Abstract CELLOIDIN embedding of human temporal bones is a time-consuming process because of the size and complexity of the specimens. The temporal bone contains numerous air spaces and delicate structures which are often not completely infiltrated by the usual methods of embedding. The present study was undertaken to develop a rapid and more efficient method of celloidin embedding of human temporal bones. Materials and Methods Eight human temporal bones, averaging 28 gm in weight and 4.3 by 3.6 by 3.0 cm in dimension, were fixed in 10% neutral buffered Formalin and decalcified in tetrasodium edetate at neutral pH as described by Gussen and Donahue.1 The end point of decalcification was determined by x-ray. The specimens were then run through graded alcohols to absolute alcohol-ether (1:1) in an autotechnicon for eight hours in each solution. The slow oscillating motion of the autotechnicon insured thorough dehydration of the specimens. Parlodion was chosen References 1. Gussen, R., and Donahue, D.: Decalcification of Temporal Bones With Tetrasodium Edetate , Arch Otolaryng 82:110, 1965.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Otolaryngology American Medical Association

Rapid Parlodion Embedding of Temporal Bones

Archives of Otolaryngology , Volume 83 (1) – Jan 1, 1966

Rapid Parlodion Embedding of Temporal Bones

Abstract

Abstract CELLOIDIN embedding of human temporal bones is a time-consuming process because of the size and complexity of the specimens. The temporal bone contains numerous air spaces and delicate structures which are often not completely infiltrated by the usual methods of embedding. The present study was undertaken to develop a rapid and more efficient method of celloidin embedding of human temporal bones. Materials and Methods Eight human temporal bones, averaging 28 gm in weight and 4.3 by...
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Publisher
American Medical Association
Copyright
Copyright © 1966 American Medical Association. All Rights Reserved.
ISSN
0003-9977
DOI
10.1001/archotol.1966.00760020030009
Publisher site
See Article on Publisher Site

Abstract

Abstract CELLOIDIN embedding of human temporal bones is a time-consuming process because of the size and complexity of the specimens. The temporal bone contains numerous air spaces and delicate structures which are often not completely infiltrated by the usual methods of embedding. The present study was undertaken to develop a rapid and more efficient method of celloidin embedding of human temporal bones. Materials and Methods Eight human temporal bones, averaging 28 gm in weight and 4.3 by 3.6 by 3.0 cm in dimension, were fixed in 10% neutral buffered Formalin and decalcified in tetrasodium edetate at neutral pH as described by Gussen and Donahue.1 The end point of decalcification was determined by x-ray. The specimens were then run through graded alcohols to absolute alcohol-ether (1:1) in an autotechnicon for eight hours in each solution. The slow oscillating motion of the autotechnicon insured thorough dehydration of the specimens. Parlodion was chosen References 1. Gussen, R., and Donahue, D.: Decalcification of Temporal Bones With Tetrasodium Edetate , Arch Otolaryng 82:110, 1965.Crossref

Journal

Archives of OtolaryngologyAmerican Medical Association

Published: Jan 1, 1966

References

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