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Randomized Controlled Trial of Adjuvant Oral Dexamethasone Pulse Therapy in Pemphigus Vulgaris

Randomized Controlled Trial of Adjuvant Oral Dexamethasone Pulse Therapy in Pemphigus Vulgaris ObjectiveTo determine the therapeutic effect of adjuvant dexamethasone pulse therapy when given in addition to conventional treatment of pemphigus vulgaris.DesignA randomized, placebo-controlled trial.SettingInternational European, multicenter outpatient and inpatient study.PatientsOf the 20 enrolled patients, 11 were randomized to the dexamethasone pulse (DP) group and 9 to the placebo pulse (PP) group.InterventionsOral dexamethasone in 300-mg pulses or PPs 3 days per month. During the intervention, the DP and PP groups received conventional treatment with prednisolone, 80 mg/d, which was tapered across 19 weeks, and azathioprine sodium, 3 mg/kg per day, until the end of the study. Monthly pulses were continued until prednisolone treatment was tapered to 0 mg.Main Outcome MeasuresNumber of patients in remission, time to and duration of remission, cumulative prednisolone dose, and occurrence of adverse events during 1 year of follow-up.ResultsEight of the 11 DP-treated patients and all 9 PP-treated patients achieved remission. Mean time to remission was 173 days with DP and 176 days with PP. The mean duration of remission within the first year was 151 days for DP and 141 days for PP. Mean cumulative prednisolone dose was 5300 mg for DP and 4882 mg for PP. Weight gain (>5% of baseline) occurred in 8 DP-treated patients compared with 1 PP-treated patient (P<.01). We found no statistically significant difference (P>.05) of an adjuvant effect of DP on remission of pemphigus vulgaris.ConclusionIn patients with new pemphigus vulgaris disease activity, there was no benefit of oral DP therapy given in addition to conventional treatment.Trial Registrationclinicaltrials.gov Identifier: NCT00127764.Pemphigus vulgaris is a potentially fatal, chronic, autoimmune mucocutaneous bullous disease, precipitated by IgG autoantibodies binding to desmoglein 3 (Dsg3) and desmoglein 1 (Dsg1).Corticosteroids (CS) form the cornerstone in the treatment of pemphigus.To minimize the iatrogenic effects of CS therapy, adjuvant treatments by CS pulse therapy were used by Pasricha et alin a large open series of 300 patients. Pulse therapy, the “big shot,” refers to the discontinuous intravenous infusion of very-high-dose CS over a short time. Pulse therapy, which was initially used for the treatment of transplant rejection, began to be used to treat pemphigus in the 1980s. Many open studies differing in design, criteria, regimen, and concomitant drugs evaluated CS pulse therapy in pemphigus.For CS pulse therapy, 500- to 1000-mg shots of methylprednisolone acetate or, most often, 100- to 200-mg shots of dexamethasone are given intravenously divided during 3 consecutive days per month.To our knowledge, there are no previous randomized controlled trials of CS pulse therapy in pemphigus. We performed a multicenter, randomized, double-blind, placebo-controlled clinical trial to evaluate the efficacy of adjuvant CS pulse therapy in addition to a conventional regimen of daily prednisolone and azathioprine in patients with new pemphigus vulgaris disease activity. The therapeutic effect was measured by the time to remission, duration of remission, cumulative prednisolone dose, and occurrence of adverse events.METHODSSTUDY PROTOCOLThe study was designed to find an additive therapeutic effect of adjuvant dexamethasone pulse (DP) therapy on remission rate and if DPs had a CS-sparing effect. The pulses were not administered as monotherapy but given in addition to an optimal conventional treatment schedule of medium-dose prednisolone plus high-dose azathioprine. The study was a European, multicenter, prospective, randomized, double-blind, placebo-controlled clinical trial. The study was initiated and coordinated by the Center for Blistering Diseases of the University Medical Center Groningen, Groningen. It was conducted from 2000 to 2005.Tablets containing dexamethasone were produced in our hospital pharmacy according to the European Pharmacopoeia. The dexamethasone was hydrophilized with methylcellulose 15 mPa · s. The tablets were prepared by direct compression using microcrystalline cellulose (Avicel PH 101; FMC BioPolymer, Philadelphia, Pa) and magnesium stearate. During pulse visits, 6 oral tablets of 50 mg of dexamethasone, or 6 tablets of placebo (which were both specially produced to be identical in taste and smell and were tested for this study)were given on 3 consecutive days every 4 weeks. The pulses were stopped when prednisolone treatment was tapered to 0 mg. If relapse occurred, prednisolone was administered (Table 1and Figure 1). Pulse dosing was reinitiated only when 80 mg of prednisolone was needed.Figure 1.Tapering schedule of a typical case with mild relapse. Arrows indicate 3-day pulses of dexamethasone.Table 1. Instruction Protocol for Prednisolone Therapy and Pulse AdjustmentStepNo. of WeeksPrednisolone Dose, mg/d*PulseDisease Activity†No (Remission)Yes&numsp;1180Start and continue every 4 weeks for 3 consecutive days; randomized to DP, 300 mg/d, or PPGo to step 2Go to step A&numsp;A1120Go to step 2Go to step B&numsp;B1240Go to step AStop&numsp;2160Go 1 step forwardGo to step 1&numsp;3140Go 1 step back&numsp;4230&numsp;5220Go 2 steps back&numsp;6215&numsp;7212.5&numsp;8210&numsp;927.510251122.5Stop prednisolone therapy12 (Remission)During remission0Stop pulseRestart at step 5 (relapse) Abbreviations: DP, dexamethasone pulse; PP, placebo pulse. *During the study, prednisolone dose was adjusted following this table using a standard definition for disease activity of skin or of mucous membrane. †Indicates treatment outcome. Disease activity was considered true with the following: (1) skin had more than 1 new pemphigus lesion per week and presence of a positive direct Nikolsky sign(sheetlike removal of nonaffected skin, at least 2 cm away from a pemphigus lesion, by pressure with a finger), or (2) old or new lesions on the mucous membrane scored as severe by the physician and pain experienced by the patient that was rated at least 5 on a scale of 0 to 10.Both study arms received conventional treatment with prednisolone and medium-high-dose azathioprine. The initial prednisolone dose was 80 mg. Tapering was performed in response to disease activity according to a predetermined schedule (Table 1and Figure 1). Azathioprine treatment was started at a dosage of 50 mg/d for 1 week and increased to 3 mg/kg per day for the rest of the study, depending on thiopurine methyltransferase activity. If the thiopurine methyltransferase level was lower than 37.5 nmol per gram of hemoglobin per hour, then the azathioprine dose was lowered to 1.5 mg/kg. The azathioprine dose was halved if leukopenia developed (<4.3 × 109leukocytes/L), decreased by 50 mg if the liver enzyme levels more than doubled, or stopped if severe thrombocytopenia developed. Prophylactic ranitidine hydrochloride and a combination of etidronate disodium and calcium carbonate or cholecalciferol (vitamin D) were given as long as CS was administered.PARTICIPATING CENTERSIn Europe the following centers participated: University Medical Center Groningen; Albert Szent-Györgyi Medical University Szeged, Szeged, Hungary; Instituto Dermopatico dell’Immacolata–IRCCS (Istituto di Ricovero e Cura a Carattere Scientifico specializzato nelle malattie della pelle), Rome; University Medical Center, Utrecht, the Netherlands; Hospital Clinic, Universitat de Barcelona, Barcelona, Spain; University Hospital Ghent, Ghent, Belgium; and Military Medical Academy of Belgrade, Belgrade. The study was approved by the medical ethical committees at each center and followed the criteria of the World Medical Association Declaration of Helsinki.SUBJECTSInclusion CriteriaPatients 18 years or older with new pemphigus vulgaris or new disease activity were enrolled. The clinical diagnosis of pemphigus vulgaris must have been verified by histological analysis and immunofluorescence microscopy of at least 1 biopsy specimen from the skin or the mucosa.Exclusion CriteriaExclusion criteria were treatment with CS within the past 2 months, treatment with adjuvant therapy other than azathioprine or having contraindications for the use of high-dose CS or azathioprine, concomitant disease treated with CS, or no ability to attend follow-up.Patients were withdrawn from the study if a serious adverse event occurred, if the investigator considered it in the best interest of the patient for safety reasons, if they withdrew their consent, or if the randomization code was broken. Patient data have been used only if the required variable data set was complete. Eligible volunteer patients signed an informed consent before entering the study.METHODS OF ASSIGNING PATIENTS TO TREATMENT GROUPSComputer-generated random numbers were used to randomize patients to the DP group or the placebo pulse (PP) group in blocks of 8. A sealed box containing the previously computer-randomized blinded study medication and case report form was provided via express mail shortly after inclusion.DETERMINATION OF SAMPLE SIZEFor this proof-of-concept study, effectiveness was defined as a difference of at least 5 weeks in the duration of remission, the primary variable. From a clinical perspective, this criterion was considered very strict, but it was chosen to achieve a more robust sample size for this multicenter study. To be able to detect this difference with a power of 90% at a 5% level of significance and an estimated standard deviation of 6 weeks, 30 patients were needed in both treatment groups.When we considered 10 weeks as the more clinically relevant difference in duration of remission for the assessment of superiority of adjuvant DP therapy compared with the conventional regimen, 9 patients in both groups would have been sufficient to conclude significance at a level of 5% with a power of 90%.SAFETY ANALYSISAll eligible patients underwent assessment of safety before inclusion in the trial. Extensive medical histories were obtained, and physical examinations were performed. A complete blood cell count, electrolyte levels, and blood glucose levels were measured and urinalysis and renal and liver function tests were performed at the initial visit, every pulse visit, and the end of study. Heart rate, blood pressure, body weight, and body temperature were recorded before and after every pulse therapy. During the first pulse visit, a chest radiograph and electrocardiogram were obtained, and blood samples for indirect immunofluorescence pemphigus titer, thiopurine methyltransferase activity, and rhesus and blood group analysis were collected. Bone density measurement for osteoporosis was performed by means of dual-energy x-ray absorptiometry scans at the first pulse and end-of-study visits.EFFICACY VARIABLESThe baseline time point was the time the patient entered the trial. The time to remission was the time between the first dose of prednisolone and the day that prednisolone treatment was tapered to 0 mg. The duration of remission was defined as the time between the prednisolone treatment taper to 0 mg and the time when there was new disease activity requiring additional prednisolone administration. The total use of prednisolone was calculated from the data in the case report form. The total number of pulses represents the number of months that dexamethasone was used during the study.DISEASE MONITORINGDuring the 1-year follow-up, assessment visits were planned every 2 weeks when pemphigus was active and every 4 weeks when the disease was in remission. Prednisolone dose was adjusted according to disease activity.Disease activity is defined as indicated in Table 1.At each visit the disease activity, compliance, medication dose and adjustment, adverse events, and safety measurements were recorded in the case report form.In each participating center, 1 trial physician recorded all of the case report forms for their patients. Prednisolone and azathioprine dose tables were faxed monthly to the trial coordination center. The adverse and serious adverse events, if any, were recorded at every visit, and appropriate action was taken, if necessary. The weight gain was calculated by comparing the initial weight with the maximum weight measured during the study.To evaluate the effect of therapy on pemphigus titer activity, serum specimens were collected at the beginning, first remission, and end of the study and were examined on antibody index level by the central coordinating laboratory at the Center for Blistering Diseases in Groningen. Therefore, the IgG indexes to Dsg1 and Dsg3 were determined by the enzyme-linked immunosorbent assay (ELISA) MESACUP desmoglein tests for Dsg1 and Dsg3 according to the protocols of the manufacturer (MBL, Nagoya, Japan).STATISTICAL ANALYSISCharacterization of Patient SampleComparability between groups was evaluated with respect to the demographic and baseline data. Baseline descriptive statistics are given as the mean (range) for continuous variables and as counts for categorical variables. To calculate differences between groups, we used the Mann-Whitney test for nonparametric continuous data and the Fisher exact test for categorical data. Kaplan-Meier survival analysis (with the log-rank test) was performed on time to remission, duration of remission, and relapses over time in the study population. In all analyses, a value of P<.05 (2 sided) was considered statistically significant. We used SPSS software, version 12 (SPSS Inc, Chicago, Ill), for statistical analysis.Sample SizeDuring the 4½ years that the study was open to accrual, we were confronted with financial and legal limitations of this trial, so that the aim of recruiting 60 patients was not reached. We decided to stop accruing patients after 4½ years and evaluate the European Randomised Placebo-Controlled Trial of Adjuvant Oral Glucocorticoid Pulse Therapy in Pemphigus (PEMPULS) using the 20 patients who were included at that time.After analysis, no statistically significant differences were observed between conventional treatment and the adjuvant regimen, raising the question of a sufficiently powered study. To further evaluate this issue, a post hoc power analysis was performed. Instead of a post hoc power estimation for differences as small as those observed in our study, we chose the clinically relevant perspective. Our small study was sufficiently powered to detect a clinically relevant difference after at least 10 weeks at a significance level of 5%.RESULTSPATIENTSFrom January 1, 2000, to June 1, 2004, 28 patients were contacted, 24 were eligible, and 20 gave informed consent and were included (Figure 2). The distribution per center was as follows: 7 patients from Groningen, 4 from Rome, 4 from Belgrade, 2 from Szeged, 1 from Utrecht, 1 from Barcelona, and 1 from Ghent. The final study follow-up assessment was performed in June 2005.Figure 2.Flowchart of patients during the study.Eleven patients received DP therapy and 9 received PP therapy. Baseline characteristics are shown in Table 2. Both groups were comparable regarding the number of lesions, age, and sex.Table 2. Baseline Demographics of Included Patients With Pemphigus VulgarisCharacteristicStudy GroupDP(n = 11)PP(n = 9)Sex, No. M/F9:24:5Age, mean (range), y46 (26-71)55 (29-67)Mean No. of skin lesions  (No. of patients)28 (7)26 (8)Mean No. of mucosal lesions  (No. of patients)7 (10)8 (9)Previous therapies, No. Systemic CS42 Topical CS10 Azathioprine11 Antibiotics20 Abbreviations: CS, corticosteroids; DP, dexamethasone pulse; PP, placebo pulse.Five patients were withdrawn from the study, including 3 from the DP group and 2 from the PP group. In the DP group, 1 patient was withdrawn because of Epstein-Barr viral hepatitis, 1 because of onset of muscle weakness and myalgia, and 1 because of cognitive impairment after pulses (steroid dementia syndrome), which was completely reversible shortly after the last pulse.In the PP group, 2 patients prematurely discontinued the study. One of these dropped out because of a newly diagnosed lung carcinoma. In retrospect, the carcinoma was not visible on the chest radiograph at the start of the study and could not be attributed to the medication in the trial. The other withdrew because of insurance problems.TIME TO REMISSIONIn the DP group, 8 of 11 patients achieved remission within 1 year. In the PP group, all 9 patients achieved remission. The mean time to remission was 173.2 days in the DP group and 175.6 days in the PP group, with a median of 141 and 140 days, respectively (Table 3).Table 3. Results of Adjuvant Dexamethasone and Placebo Treatment in Patients With Pemphigus VulgarisFindingStudy Group*PValueDPPPNo. of pulses until first remission  or end of follow-up5.44 (9)†6.44 (9).35Time to first remission, d  (or 365 d)‡173.2 (10)175.6 (9).76Survival of first remission to relapse  or to end of follow-up, d137 (9)122 (9).64No. of days of prednisolone use  during 1 y214 (9)224 (8).55Remission during 1 y, d151 (8)141 (8).82Remission until relapse, d102 (4)26 (3).06§Prednisolone dose to reach  remission, mg3654 (8)4329 (9).50Cumulative prednisolone dose  during 1 y, mg5300 (8)4882 (7).78 Abbreviations: DP, dexamethasone pulse; PP, placebo pulse. *Data are expressed as mean (number of patients). †Data were not available from all patients because some end points were never reached by these patients. ‡In patients who reached no remission by the end of the study, 365 days were calculated. §Pvalue applies only to patients who relapsed during the study. When the censoring of patients who did not relapse was accounted for, P = .88.An optimal response of remission within 19 weeks was found in 4 patients receiving DP therapy and 6 patients receiving PP therapy. The difference between the times each group took to achieve remission (1-2 days) was not significant (P = .76).DURATION OF REMISSIONThe mean number of days in remission until day 365 was 151 (range, 0-233) in the DP group, and 141 (range, 8-232) in the PP group. The difference of 10 days in duration of remission was statistically not significant (P = .82) (Table 3). Within the follow-up period, relapses after remission occurred in 4 patients in the DP group and 3 patients in the PP group. The mean time from remission to relapse before the end of the study was 102 days (range, 62-166 days) in the DP group and 26 days (range, 6-65 days) in the PP group. The trend of a longer relapse-free remission in the DP group was not statistically significant owing to the very small numbers of patients in this subset.CUMULATIVE CS USE AFTER 1 YEARThe mean cumulative prednisolone dose was 5300 mg in the DP group and 4882 mg in the PP group (Table 3) (P = .78). The number of days of prednisolone use in the DP group was 198; in the PP, group 224. The average daily dose of prednisolone was 24.8 mg in the DP group and 21.8 mg in the PP group (Figure 3).Figure 3.Mean doses of prednisolone used by the study groups. A, Mean doses for both groups during the study. These means were strongly influenced by individual extremes because of the small group sizes. B and C, Individual prednisolone doses in the dexamethasone pulse (DP) and placebo pulse (PP) groups, respectively, during the study. Thicker lines indicate more patients receiving the same dosage.The mean dose of prednisolone needed to achieve remission was lower in the DP group (3654 mg) than in the PP group (4329 mg), counting only those patients who achieved remission. The trend was statistically not significant. Patients in the DP and PP groups received a mean of 5.44 and 6.44 pulse courses, respectively, before the first remission.ADVERSE EVENTS AND TOLERABILITYIn the DP group, 30 adverse events occurred in 10 of 11 patients compared with 14 adverse events in 5 of 9 patients in the PP group (Table 4). Three patients in the DP group discontinued the study because of adverse events in the form of infection, myalgia, high blood glucose levels, and cognitive dysfunction. All adverse events disappeared after discontinuation of medication therapy. A greater than 5% weight gain was encountered by 8 of 9 patients in the DP group and in 1 of 9 patients in the PP group. The mean increase in body weight compared with the initial weight was 12.4% in the DP group compared with 2.9% (P = .01) in the PP group. After discontinuation of CS therapy, weight gain had the tendency to resolve. Adjustment of azathioprine dose, treatment of infection, and use of elastic stockings and antithrombotic agents controlled or prevented many of the adverse effects.Table 4. Adverse Events*Adverse EventStudy Group, No. of PatientsDP(n = 11)PP(n = 9)Weight gain†81Increased blood glucose level11Wound infection1Urinary tract infection1EBV hepatitis‡1Oral Candidaspecies infection1Nausea1Diarrhea2Myopathy2Cushingoid effect2Acne1Hypertrichosis1Facial flushing1Furuncle1Lentiginosis1Hypertension11Deep venous thrombosis11Lung tumor and metastasis‡1Coughing after azathioprine therapy1Concentration and memory disorders1Peroneal lesion1Leukopenia2Anemia1Kidney function disorder1Liver function disorders12Glaucoma1Postherpetic neuralgia§1 Abbreviations: DP, dexamethasone pulse; EBV, Epstein-Barr virus; PP, placebo pulse. *Adverse events occurred in 10 patients in the DP group and 5 patients in the PP group. Some patients experienced more than 1 event. †Indicates weight gain of at least 1 period during complete follow-up compared with total body weight. ‡Indicates severe adverse events. §Occurs after stopping prednisolone therapy.ANTIBODY LEVEL IN DIFFERENT STAGES OF THE STUDYIn the DP group, the mean IgG against Dsg1 ELISA indexes of serum samples collected at the beginning, remission, and end of study were 33 (n = 8), 1 (n = 6), and 2 (n = 7), respectively. In the PP group, these were 52 (n = 7), 6 (n = 6), and 25 (n = 7), respectively. In the DP group, the mean of IgG against Dsg3 ELISA indexes of the same serum samples collected at the beginning, remission, and end of study were 143, 53, and 74, respectively. In the PP group, these were 175, 102, and 129, respectively. The reference value of the anti-Dsg1 index is less than 14; of the anti-Dsg3 index, less than 7.COMMENTWe believe this to be the first time that a randomized, double-blind, placebo-controlled trial has been conducted in pemphigus vulgaris. We were unable to prove whether oral DP therapy had an additive beneficial effect compared with a conventional regimen of prednisolone, 80 mg, with a tapering schedule, and azathioprine, 3 mg/kg per day, in terms of time to remission, duration of remission, or cumulative prednisolone dose. The number of patients included in this study was only one third that of the preplanned number of patients; therefore, small benefits of less than 10 weeks' extension of remission might have been missed. However, we believe that from a clinical perspective, these differences would not have altered the decision to choose not to initiate oral DP therapy. In addition, a post hoc power analysis showed that our small study was sufficiently powered for a clinically relevant difference of at least 10 weeks. The trend of a longer relapse-free remission time in the DP group (102 days) vs the PP group (26 days) was based on subsets of only 4 and 3 patients, respectively. We did not see any relapses in the 12 patients who achieved remission at later than 141 days of prednisolone treatment, most likely because of the short follow-up period of 365 days. It is therefore hard to judge whether the difference in duration of the remission between the groups is a trend or merely a coincidence.A positive trend, which was statistically not significant, was the lower mean prednisolone dose used to achieve remission in the DP group. We should remember, however, that in this trial a very quick tapering of the prednisolone dose to 0 mg within 19 weeks was chosen to detect early relapses in the control group. The positive trends in the DP group might have been leveled if prednisolone treatment had not been tapered to less than 10 mg/d in both groups, as is often used in practice. However, relapses occurring in this quick tapering schedule could be controlled with stepped-up doses of 20 or 40 mg of prednisolone.The results of this randomized controlled trial cannot directly be compared with those of other open patient series in which DP was used in combination with cyclophosphamide, usually called dexamethasone-cyclophosphamide pulse (DCP) therapy.In DCP therapy, patients receive low daily doses of oral prednisolone and cyclophosphamide, 50 mg/d.We chose azathioprine instead of cyclophosphamide as the CS-sparing agent because the latter has the potential to induce malignanciesand might result in permanent sterilization in women. The surprisingly good results of the PP (control) group in this study might be attributed to the continued azathioprine administration during the study at a higher dosage (3 mg/kg per day) than is commonly used (<2 mg/kg per day). Randomized placebo-controlled studies addressing the effect of azathioprine in pemphigus are lacking, although its CS-sparing effect has been shown in other autoimmune bullous diseases.A second difference compared with the DCP study is that we stopped pulses after tapering prednisolone treatment to 0 mg, whereas DCP therapy was continued for 6 months. A third difference is that we used oral instead of intravenous CS pulses. Oral pulses are more convenient and serve as a more acceptable blinding method for placebo. Oral dexamethasone pulsing has a bioavailability of 60%.In this study, only patients with newly diagnosed pemphigus vulgaris or those in complete remission with a new flare-up were included, so the results do not extend to patients with refractory disease that is resistant to conventional therapy. Adjuvant pulse therapy may have a value for severe or refractory cases of pemphigus.We saw a significantly larger weight gain in patients in the DP group. This weight gain was probably due to cushingoid obesity, which is a known to be an adverse effect of CS therapy. In this study, a small number of adverse events were probably caused by azathioprine, such as liver and kidney dysfunction, anemia, and leukopenia. These effects were reversible after lowering the azathioprine dose. The end-of-study dual-energy x-ray absorptiometry scans showed no signs of osteoporosis in any patient.With regard to the anti-Dsg1 and anti-Dsg3 ELISA results, both study groups showed a significant decline between the start and remission levels of Dsg1 antibodies, and also, but to a lesser extent, in Dsg3 antibodies. After discontinuation of CS therapy, a slight increase in Dsg3 antibody levels was seen in both groups, but in Dsg1 antibody levels only in the PP group. We could not find a statistically significant difference in reduction of antibody levels between the groups.As we stated, the conclusions that can be drawn from this study for the treatment of patients with pemphigus vulgaris are somewhat restricted because of the small number of patients (n = 20), the relatively short follow-up period (365 days), and the restriction to patients with new disease activity. However, we believe that when the observed treatment differences are considered from a clinical respective, these restrictions do not alter our overall recommendation against using DP therapy in this patient group. The results from both groups imply that conventional treatment with prednisolone, 80 mg/d, on a tapering schedule to 0 mg in 19 weeks, and azathioprine sodium, 3 mg/kg, continued for 1 year after tapering, is an effective therapy for new disease activity in patients with pemphigus vulgaris, and that adding DP therapy to this regimen has no benefit.Correspondence:Marcel F. Jonkman, MD, PhD, Department of Dermatology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, the Netherlands (m.f.jonkman@derm.umcg.nl).Accepted for Publication:January 19, 2006.Author Affiliations:Center for Blistering Diseases, Department of Dermatology (Drs Mentink, Mackenzie, Tóth, and Jonkman), Department of Pharmacy (Dr Laseur), and Trial Coordinations Center (Messrs Lambert and Veeger), University Medical Center Groningen, University of Groningen, Groningen, the Netherlands; Department of Immunodermatology, Instituto Dermopatico dell’Immacolata-IRCCS, Rome, Italy (Dr Cianchini); and Department of Dermatology, Military Medical Academy, Belgrade, Serbia, and Montenegro (Dr Pavlovi&cacute;). Dr Mackenzie is now with the Department of Dermatology, University Medical Center St Radboud, Nijmegen, the Netherlands; Dr Tóth, Department of Dermatology, Martini Hospital, Groningen; and Mr Lambert, PharmaBio-Research, Groningen.Author Contributions:The authors had full access to the data and were the sole preparers of this manuscript. Study concept and design: Mentink, Tóth, Laseur, Lambert, and Jonkman. Acquisition of data: Mentink, Mackenzie, Tóth, Lambert, Cianchini, and Pavlovi&cacute;. Analysis and interpretation of data: Mentink and Veeger. Drafting of the manuscript: Mentink and Jonkman. Critical revision of the manuscript for important intellectual content: Mentink, Veeger, and Jonkman. Statistical analysis: Veeger. Obtained funding: Tóth and Jonkman. Administrative, technical, and material support: Mentink, Mackenzie, Tóth, Laseur, Lambert, and Pavlovi&cacute;. Study supervision and drafting of revised manuscript: Jonkman.Financial Disclosure:None.Funding/Support:This study was supported by a research grant from the University Medical Center Groningen, Groningen, the Netherlands.Previous Presentations:This study was presented at the 35th Annual Meeting of the European Sociey for Dermatological Research; September 22, 2005; Tübingen, Germany; and at the Annual Meeting of the Medical Dermatology Society; March 2, 2006; San Francisco, Calif.Additional Information:This manuscript was not prepared by someone other than the authors.Acknowledgment:We thank the following colleagues for voluntarily recruiting and treating patients for this study: Sandor Husz, MD (Hungary), Koos Sanders, MD (the Netherlands), Pilar Iranzo, MD (Spain), Hilde Beele, MD, and Barbara Boone, MD (Belgium); for data entry: Marco Assmann, MSc; for thiopurine methyltransferase analysis: Ido Kema, PhD; for pharmaceutical support: Ida Sikkema, RPh; for desmogleins ELISA: Hendri Pas, PhD; and for linguistic correction of the manuscript: Dédée Murrell, MD (Sydney, New South Wales).This article was corrected on 5/23/06, prior to publication of the correction in print.REFERENCESJCBystrynJLRudolphPemphigus.Lancet2005366617315993235KEHarmanSAlbertMMBlackGuidelines for the management of pemphigus vulgaris.Br J Dermatol200314992693714632796JSPasrichaBKKhaitanRSRamanMChandraDexamethasone-cyclophosphamide pulse therapy for pemphigus.Int J Dermatol1995348758828647673JSPasrichaJThanzamaUKKhanIntermittent high-dose dexamethasone-cyclophosphamide therapy for pemphigus.Br J Dermatol198811973773408666SKaurAJKanwarDexamethasone-cyclophosphamide pulse therapy in pemphigus.Int J Dermatol1990293713742361797JSPasrichaSSDasCurative effect of dexamethasone-cyclophosphamide pulse therapy for the treatment of pemphigus vulgaris.Int J Dermatol1992318758771478770FChryssomallisADimitriadesGCChaidemenosDPanagiotidesGKarakatsanisSteroid-pulse therapy in pemphigus vulgaris long term follow-up.Int J Dermatol1995344384427657447JSPasrichaBKKhaitanCurative treatment for pemphigus.Arch Dermatol1996132151815198961890VPWerthTreatment of pemphigus vulgaris with brief, high-dose intravenous glucocorticoids.Arch Dermatol1996132143514398961871FFemianoFGombosCScullyPemphigus vulgaris with oral involvement.J Eur Acad Dermatol Venereol20021635335612224691AJKanwarSKaurGPThamiLong-term efficacy of dexamethasone-cyclophosphamide pulse therapy in pemphigus.Dermatology200220422823112037452MDMignognaLLo MuzioERuoppoSFedeleLLo RussoEBucciHigh-dose intravenous “pulse” methylprednisone in the treatment of severe oropharyngeal pemphigus: a pilot study.J Oral Pathol Med20023133934412201245GGTothJBvan de MeerMFJonkmanDexamethasone pulse therapy in pemphigus.J Eur Acad Dermatol Venereol20021660761112482045RJainBKumarImmediate and delayed complications of dexamethasone cyclophosphamide pulse (DCP) therapy.J Dermatol20033071371814684952SSacchidanandNCHiremathHVNatrajDexamethasone-cyclophosphamide pulse therapy for autoimmune-vesiculobullous disorders at Victoria hospital, Bangalore.Dermatol Online J200392http://dermatology.cdlib.org/95/original/pemphigus/sacchidanand.html. Accessed September 5, 2005ERoseSWeverDZillikensRLinseUFHausteinEBBrockerIntravenous dexamethasone-cyclophosphamide pulse therapy in comparison with oral methylprednisolone-azathioprine therapy in patients with pemphigus: results of a multicenter prospectively randomized study.J Dtsch Dermatol Ges2005320020616372814RJainHBaliVKSharmaBKumarCardiovascular effects of corticosteroid pulse therapy.Int J Dermatol20054428528815811078GGTothCKloostermanDRUgesMFJonkmanPharmacokinetics of high-dose oral and intravenous dexamethasone.Ther Drug Monit19992153253510519450SAGrandoAAGrandoBTGlukhenkyVDoguzovVTNguyenKHolubarHistory and clinical significance of mechanical symptoms in blistering dermatoses: a reappraisal.J Am Acad Dermatol200348869212522376OSacksMShulmanSteroid dementia.Neurology20056470770915728296VKMahajanNLSharmaRCSharmaGGargTwelve-year clinico-therapeutic experience in pemphigus: a retrospective study of 54 cases.Int J Dermatol20054482182716207182CTalar-WilliamsYMHijaziMMWaltherCyclophosphamide-induced cystitis and bladder cancer in patients with Wegener granulomatosis.Ann Intern Med19961244774848602705JLBurtonRRHarmanRDPeacheyRPWarinAzathioprine plus prednisone in treatment of pemphigoid.BMJ1978211901191363229WFLeverGSchaumburg-LeverTreatment of pemphigus vulgaris: results obtained in 84 patients between 1961 and 1982.Arch Dermatol198412044476691714WAbererECWolf-SchreinerGStinglKWolffAzathioprine in the treatment of pemphigus vulgaris.J Am Acad Dermatol1987165275333819096 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png JAMA Dermatology American Medical Association

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Copyright 2006 American Medical Association. All Rights Reserved. Applicable FARS/DFARS Restrictions Apply to Government Use.
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Abstract

ObjectiveTo determine the therapeutic effect of adjuvant dexamethasone pulse therapy when given in addition to conventional treatment of pemphigus vulgaris.DesignA randomized, placebo-controlled trial.SettingInternational European, multicenter outpatient and inpatient study.PatientsOf the 20 enrolled patients, 11 were randomized to the dexamethasone pulse (DP) group and 9 to the placebo pulse (PP) group.InterventionsOral dexamethasone in 300-mg pulses or PPs 3 days per month. During the intervention, the DP and PP groups received conventional treatment with prednisolone, 80 mg/d, which was tapered across 19 weeks, and azathioprine sodium, 3 mg/kg per day, until the end of the study. Monthly pulses were continued until prednisolone treatment was tapered to 0 mg.Main Outcome MeasuresNumber of patients in remission, time to and duration of remission, cumulative prednisolone dose, and occurrence of adverse events during 1 year of follow-up.ResultsEight of the 11 DP-treated patients and all 9 PP-treated patients achieved remission. Mean time to remission was 173 days with DP and 176 days with PP. The mean duration of remission within the first year was 151 days for DP and 141 days for PP. Mean cumulative prednisolone dose was 5300 mg for DP and 4882 mg for PP. Weight gain (>5% of baseline) occurred in 8 DP-treated patients compared with 1 PP-treated patient (P<.01). We found no statistically significant difference (P>.05) of an adjuvant effect of DP on remission of pemphigus vulgaris.ConclusionIn patients with new pemphigus vulgaris disease activity, there was no benefit of oral DP therapy given in addition to conventional treatment.Trial Registrationclinicaltrials.gov Identifier: NCT00127764.Pemphigus vulgaris is a potentially fatal, chronic, autoimmune mucocutaneous bullous disease, precipitated by IgG autoantibodies binding to desmoglein 3 (Dsg3) and desmoglein 1 (Dsg1).Corticosteroids (CS) form the cornerstone in the treatment of pemphigus.To minimize the iatrogenic effects of CS therapy, adjuvant treatments by CS pulse therapy were used by Pasricha et alin a large open series of 300 patients. Pulse therapy, the “big shot,” refers to the discontinuous intravenous infusion of very-high-dose CS over a short time. Pulse therapy, which was initially used for the treatment of transplant rejection, began to be used to treat pemphigus in the 1980s. Many open studies differing in design, criteria, regimen, and concomitant drugs evaluated CS pulse therapy in pemphigus.For CS pulse therapy, 500- to 1000-mg shots of methylprednisolone acetate or, most often, 100- to 200-mg shots of dexamethasone are given intravenously divided during 3 consecutive days per month.To our knowledge, there are no previous randomized controlled trials of CS pulse therapy in pemphigus. We performed a multicenter, randomized, double-blind, placebo-controlled clinical trial to evaluate the efficacy of adjuvant CS pulse therapy in addition to a conventional regimen of daily prednisolone and azathioprine in patients with new pemphigus vulgaris disease activity. The therapeutic effect was measured by the time to remission, duration of remission, cumulative prednisolone dose, and occurrence of adverse events.METHODSSTUDY PROTOCOLThe study was designed to find an additive therapeutic effect of adjuvant dexamethasone pulse (DP) therapy on remission rate and if DPs had a CS-sparing effect. The pulses were not administered as monotherapy but given in addition to an optimal conventional treatment schedule of medium-dose prednisolone plus high-dose azathioprine. The study was a European, multicenter, prospective, randomized, double-blind, placebo-controlled clinical trial. The study was initiated and coordinated by the Center for Blistering Diseases of the University Medical Center Groningen, Groningen. It was conducted from 2000 to 2005.Tablets containing dexamethasone were produced in our hospital pharmacy according to the European Pharmacopoeia. The dexamethasone was hydrophilized with methylcellulose 15 mPa · s. The tablets were prepared by direct compression using microcrystalline cellulose (Avicel PH 101; FMC BioPolymer, Philadelphia, Pa) and magnesium stearate. During pulse visits, 6 oral tablets of 50 mg of dexamethasone, or 6 tablets of placebo (which were both specially produced to be identical in taste and smell and were tested for this study)were given on 3 consecutive days every 4 weeks. The pulses were stopped when prednisolone treatment was tapered to 0 mg. If relapse occurred, prednisolone was administered (Table 1and Figure 1). Pulse dosing was reinitiated only when 80 mg of prednisolone was needed.Figure 1.Tapering schedule of a typical case with mild relapse. Arrows indicate 3-day pulses of dexamethasone.Table 1. Instruction Protocol for Prednisolone Therapy and Pulse AdjustmentStepNo. of WeeksPrednisolone Dose, mg/d*PulseDisease Activity†No (Remission)Yes&numsp;1180Start and continue every 4 weeks for 3 consecutive days; randomized to DP, 300 mg/d, or PPGo to step 2Go to step A&numsp;A1120Go to step 2Go to step B&numsp;B1240Go to step AStop&numsp;2160Go 1 step forwardGo to step 1&numsp;3140Go 1 step back&numsp;4230&numsp;5220Go 2 steps back&numsp;6215&numsp;7212.5&numsp;8210&numsp;927.510251122.5Stop prednisolone therapy12 (Remission)During remission0Stop pulseRestart at step 5 (relapse) Abbreviations: DP, dexamethasone pulse; PP, placebo pulse. *During the study, prednisolone dose was adjusted following this table using a standard definition for disease activity of skin or of mucous membrane. †Indicates treatment outcome. Disease activity was considered true with the following: (1) skin had more than 1 new pemphigus lesion per week and presence of a positive direct Nikolsky sign(sheetlike removal of nonaffected skin, at least 2 cm away from a pemphigus lesion, by pressure with a finger), or (2) old or new lesions on the mucous membrane scored as severe by the physician and pain experienced by the patient that was rated at least 5 on a scale of 0 to 10.Both study arms received conventional treatment with prednisolone and medium-high-dose azathioprine. The initial prednisolone dose was 80 mg. Tapering was performed in response to disease activity according to a predetermined schedule (Table 1and Figure 1). Azathioprine treatment was started at a dosage of 50 mg/d for 1 week and increased to 3 mg/kg per day for the rest of the study, depending on thiopurine methyltransferase activity. If the thiopurine methyltransferase level was lower than 37.5 nmol per gram of hemoglobin per hour, then the azathioprine dose was lowered to 1.5 mg/kg. The azathioprine dose was halved if leukopenia developed (<4.3 × 109leukocytes/L), decreased by 50 mg if the liver enzyme levels more than doubled, or stopped if severe thrombocytopenia developed. Prophylactic ranitidine hydrochloride and a combination of etidronate disodium and calcium carbonate or cholecalciferol (vitamin D) were given as long as CS was administered.PARTICIPATING CENTERSIn Europe the following centers participated: University Medical Center Groningen; Albert Szent-Györgyi Medical University Szeged, Szeged, Hungary; Instituto Dermopatico dell’Immacolata–IRCCS (Istituto di Ricovero e Cura a Carattere Scientifico specializzato nelle malattie della pelle), Rome; University Medical Center, Utrecht, the Netherlands; Hospital Clinic, Universitat de Barcelona, Barcelona, Spain; University Hospital Ghent, Ghent, Belgium; and Military Medical Academy of Belgrade, Belgrade. The study was approved by the medical ethical committees at each center and followed the criteria of the World Medical Association Declaration of Helsinki.SUBJECTSInclusion CriteriaPatients 18 years or older with new pemphigus vulgaris or new disease activity were enrolled. The clinical diagnosis of pemphigus vulgaris must have been verified by histological analysis and immunofluorescence microscopy of at least 1 biopsy specimen from the skin or the mucosa.Exclusion CriteriaExclusion criteria were treatment with CS within the past 2 months, treatment with adjuvant therapy other than azathioprine or having contraindications for the use of high-dose CS or azathioprine, concomitant disease treated with CS, or no ability to attend follow-up.Patients were withdrawn from the study if a serious adverse event occurred, if the investigator considered it in the best interest of the patient for safety reasons, if they withdrew their consent, or if the randomization code was broken. Patient data have been used only if the required variable data set was complete. Eligible volunteer patients signed an informed consent before entering the study.METHODS OF ASSIGNING PATIENTS TO TREATMENT GROUPSComputer-generated random numbers were used to randomize patients to the DP group or the placebo pulse (PP) group in blocks of 8. A sealed box containing the previously computer-randomized blinded study medication and case report form was provided via express mail shortly after inclusion.DETERMINATION OF SAMPLE SIZEFor this proof-of-concept study, effectiveness was defined as a difference of at least 5 weeks in the duration of remission, the primary variable. From a clinical perspective, this criterion was considered very strict, but it was chosen to achieve a more robust sample size for this multicenter study. To be able to detect this difference with a power of 90% at a 5% level of significance and an estimated standard deviation of 6 weeks, 30 patients were needed in both treatment groups.When we considered 10 weeks as the more clinically relevant difference in duration of remission for the assessment of superiority of adjuvant DP therapy compared with the conventional regimen, 9 patients in both groups would have been sufficient to conclude significance at a level of 5% with a power of 90%.SAFETY ANALYSISAll eligible patients underwent assessment of safety before inclusion in the trial. Extensive medical histories were obtained, and physical examinations were performed. A complete blood cell count, electrolyte levels, and blood glucose levels were measured and urinalysis and renal and liver function tests were performed at the initial visit, every pulse visit, and the end of study. Heart rate, blood pressure, body weight, and body temperature were recorded before and after every pulse therapy. During the first pulse visit, a chest radiograph and electrocardiogram were obtained, and blood samples for indirect immunofluorescence pemphigus titer, thiopurine methyltransferase activity, and rhesus and blood group analysis were collected. Bone density measurement for osteoporosis was performed by means of dual-energy x-ray absorptiometry scans at the first pulse and end-of-study visits.EFFICACY VARIABLESThe baseline time point was the time the patient entered the trial. The time to remission was the time between the first dose of prednisolone and the day that prednisolone treatment was tapered to 0 mg. The duration of remission was defined as the time between the prednisolone treatment taper to 0 mg and the time when there was new disease activity requiring additional prednisolone administration. The total use of prednisolone was calculated from the data in the case report form. The total number of pulses represents the number of months that dexamethasone was used during the study.DISEASE MONITORINGDuring the 1-year follow-up, assessment visits were planned every 2 weeks when pemphigus was active and every 4 weeks when the disease was in remission. Prednisolone dose was adjusted according to disease activity.Disease activity is defined as indicated in Table 1.At each visit the disease activity, compliance, medication dose and adjustment, adverse events, and safety measurements were recorded in the case report form.In each participating center, 1 trial physician recorded all of the case report forms for their patients. Prednisolone and azathioprine dose tables were faxed monthly to the trial coordination center. The adverse and serious adverse events, if any, were recorded at every visit, and appropriate action was taken, if necessary. The weight gain was calculated by comparing the initial weight with the maximum weight measured during the study.To evaluate the effect of therapy on pemphigus titer activity, serum specimens were collected at the beginning, first remission, and end of the study and were examined on antibody index level by the central coordinating laboratory at the Center for Blistering Diseases in Groningen. Therefore, the IgG indexes to Dsg1 and Dsg3 were determined by the enzyme-linked immunosorbent assay (ELISA) MESACUP desmoglein tests for Dsg1 and Dsg3 according to the protocols of the manufacturer (MBL, Nagoya, Japan).STATISTICAL ANALYSISCharacterization of Patient SampleComparability between groups was evaluated with respect to the demographic and baseline data. Baseline descriptive statistics are given as the mean (range) for continuous variables and as counts for categorical variables. To calculate differences between groups, we used the Mann-Whitney test for nonparametric continuous data and the Fisher exact test for categorical data. Kaplan-Meier survival analysis (with the log-rank test) was performed on time to remission, duration of remission, and relapses over time in the study population. In all analyses, a value of P<.05 (2 sided) was considered statistically significant. We used SPSS software, version 12 (SPSS Inc, Chicago, Ill), for statistical analysis.Sample SizeDuring the 4½ years that the study was open to accrual, we were confronted with financial and legal limitations of this trial, so that the aim of recruiting 60 patients was not reached. We decided to stop accruing patients after 4½ years and evaluate the European Randomised Placebo-Controlled Trial of Adjuvant Oral Glucocorticoid Pulse Therapy in Pemphigus (PEMPULS) using the 20 patients who were included at that time.After analysis, no statistically significant differences were observed between conventional treatment and the adjuvant regimen, raising the question of a sufficiently powered study. To further evaluate this issue, a post hoc power analysis was performed. Instead of a post hoc power estimation for differences as small as those observed in our study, we chose the clinically relevant perspective. Our small study was sufficiently powered to detect a clinically relevant difference after at least 10 weeks at a significance level of 5%.RESULTSPATIENTSFrom January 1, 2000, to June 1, 2004, 28 patients were contacted, 24 were eligible, and 20 gave informed consent and were included (Figure 2). The distribution per center was as follows: 7 patients from Groningen, 4 from Rome, 4 from Belgrade, 2 from Szeged, 1 from Utrecht, 1 from Barcelona, and 1 from Ghent. The final study follow-up assessment was performed in June 2005.Figure 2.Flowchart of patients during the study.Eleven patients received DP therapy and 9 received PP therapy. Baseline characteristics are shown in Table 2. Both groups were comparable regarding the number of lesions, age, and sex.Table 2. Baseline Demographics of Included Patients With Pemphigus VulgarisCharacteristicStudy GroupDP(n = 11)PP(n = 9)Sex, No. M/F9:24:5Age, mean (range), y46 (26-71)55 (29-67)Mean No. of skin lesions  (No. of patients)28 (7)26 (8)Mean No. of mucosal lesions  (No. of patients)7 (10)8 (9)Previous therapies, No. Systemic CS42 Topical CS10 Azathioprine11 Antibiotics20 Abbreviations: CS, corticosteroids; DP, dexamethasone pulse; PP, placebo pulse.Five patients were withdrawn from the study, including 3 from the DP group and 2 from the PP group. In the DP group, 1 patient was withdrawn because of Epstein-Barr viral hepatitis, 1 because of onset of muscle weakness and myalgia, and 1 because of cognitive impairment after pulses (steroid dementia syndrome), which was completely reversible shortly after the last pulse.In the PP group, 2 patients prematurely discontinued the study. One of these dropped out because of a newly diagnosed lung carcinoma. In retrospect, the carcinoma was not visible on the chest radiograph at the start of the study and could not be attributed to the medication in the trial. The other withdrew because of insurance problems.TIME TO REMISSIONIn the DP group, 8 of 11 patients achieved remission within 1 year. In the PP group, all 9 patients achieved remission. The mean time to remission was 173.2 days in the DP group and 175.6 days in the PP group, with a median of 141 and 140 days, respectively (Table 3).Table 3. Results of Adjuvant Dexamethasone and Placebo Treatment in Patients With Pemphigus VulgarisFindingStudy Group*PValueDPPPNo. of pulses until first remission  or end of follow-up5.44 (9)†6.44 (9).35Time to first remission, d  (or 365 d)‡173.2 (10)175.6 (9).76Survival of first remission to relapse  or to end of follow-up, d137 (9)122 (9).64No. of days of prednisolone use  during 1 y214 (9)224 (8).55Remission during 1 y, d151 (8)141 (8).82Remission until relapse, d102 (4)26 (3).06§Prednisolone dose to reach  remission, mg3654 (8)4329 (9).50Cumulative prednisolone dose  during 1 y, mg5300 (8)4882 (7).78 Abbreviations: DP, dexamethasone pulse; PP, placebo pulse. *Data are expressed as mean (number of patients). †Data were not available from all patients because some end points were never reached by these patients. ‡In patients who reached no remission by the end of the study, 365 days were calculated. §Pvalue applies only to patients who relapsed during the study. When the censoring of patients who did not relapse was accounted for, P = .88.An optimal response of remission within 19 weeks was found in 4 patients receiving DP therapy and 6 patients receiving PP therapy. The difference between the times each group took to achieve remission (1-2 days) was not significant (P = .76).DURATION OF REMISSIONThe mean number of days in remission until day 365 was 151 (range, 0-233) in the DP group, and 141 (range, 8-232) in the PP group. The difference of 10 days in duration of remission was statistically not significant (P = .82) (Table 3). Within the follow-up period, relapses after remission occurred in 4 patients in the DP group and 3 patients in the PP group. The mean time from remission to relapse before the end of the study was 102 days (range, 62-166 days) in the DP group and 26 days (range, 6-65 days) in the PP group. The trend of a longer relapse-free remission in the DP group was not statistically significant owing to the very small numbers of patients in this subset.CUMULATIVE CS USE AFTER 1 YEARThe mean cumulative prednisolone dose was 5300 mg in the DP group and 4882 mg in the PP group (Table 3) (P = .78). The number of days of prednisolone use in the DP group was 198; in the PP, group 224. The average daily dose of prednisolone was 24.8 mg in the DP group and 21.8 mg in the PP group (Figure 3).Figure 3.Mean doses of prednisolone used by the study groups. A, Mean doses for both groups during the study. These means were strongly influenced by individual extremes because of the small group sizes. B and C, Individual prednisolone doses in the dexamethasone pulse (DP) and placebo pulse (PP) groups, respectively, during the study. Thicker lines indicate more patients receiving the same dosage.The mean dose of prednisolone needed to achieve remission was lower in the DP group (3654 mg) than in the PP group (4329 mg), counting only those patients who achieved remission. The trend was statistically not significant. Patients in the DP and PP groups received a mean of 5.44 and 6.44 pulse courses, respectively, before the first remission.ADVERSE EVENTS AND TOLERABILITYIn the DP group, 30 adverse events occurred in 10 of 11 patients compared with 14 adverse events in 5 of 9 patients in the PP group (Table 4). Three patients in the DP group discontinued the study because of adverse events in the form of infection, myalgia, high blood glucose levels, and cognitive dysfunction. All adverse events disappeared after discontinuation of medication therapy. A greater than 5% weight gain was encountered by 8 of 9 patients in the DP group and in 1 of 9 patients in the PP group. The mean increase in body weight compared with the initial weight was 12.4% in the DP group compared with 2.9% (P = .01) in the PP group. After discontinuation of CS therapy, weight gain had the tendency to resolve. Adjustment of azathioprine dose, treatment of infection, and use of elastic stockings and antithrombotic agents controlled or prevented many of the adverse effects.Table 4. Adverse Events*Adverse EventStudy Group, No. of PatientsDP(n = 11)PP(n = 9)Weight gain†81Increased blood glucose level11Wound infection1Urinary tract infection1EBV hepatitis‡1Oral Candidaspecies infection1Nausea1Diarrhea2Myopathy2Cushingoid effect2Acne1Hypertrichosis1Facial flushing1Furuncle1Lentiginosis1Hypertension11Deep venous thrombosis11Lung tumor and metastasis‡1Coughing after azathioprine therapy1Concentration and memory disorders1Peroneal lesion1Leukopenia2Anemia1Kidney function disorder1Liver function disorders12Glaucoma1Postherpetic neuralgia§1 Abbreviations: DP, dexamethasone pulse; EBV, Epstein-Barr virus; PP, placebo pulse. *Adverse events occurred in 10 patients in the DP group and 5 patients in the PP group. Some patients experienced more than 1 event. †Indicates weight gain of at least 1 period during complete follow-up compared with total body weight. ‡Indicates severe adverse events. §Occurs after stopping prednisolone therapy.ANTIBODY LEVEL IN DIFFERENT STAGES OF THE STUDYIn the DP group, the mean IgG against Dsg1 ELISA indexes of serum samples collected at the beginning, remission, and end of study were 33 (n = 8), 1 (n = 6), and 2 (n = 7), respectively. In the PP group, these were 52 (n = 7), 6 (n = 6), and 25 (n = 7), respectively. In the DP group, the mean of IgG against Dsg3 ELISA indexes of the same serum samples collected at the beginning, remission, and end of study were 143, 53, and 74, respectively. In the PP group, these were 175, 102, and 129, respectively. The reference value of the anti-Dsg1 index is less than 14; of the anti-Dsg3 index, less than 7.COMMENTWe believe this to be the first time that a randomized, double-blind, placebo-controlled trial has been conducted in pemphigus vulgaris. We were unable to prove whether oral DP therapy had an additive beneficial effect compared with a conventional regimen of prednisolone, 80 mg, with a tapering schedule, and azathioprine, 3 mg/kg per day, in terms of time to remission, duration of remission, or cumulative prednisolone dose. The number of patients included in this study was only one third that of the preplanned number of patients; therefore, small benefits of less than 10 weeks' extension of remission might have been missed. However, we believe that from a clinical perspective, these differences would not have altered the decision to choose not to initiate oral DP therapy. In addition, a post hoc power analysis showed that our small study was sufficiently powered for a clinically relevant difference of at least 10 weeks. The trend of a longer relapse-free remission time in the DP group (102 days) vs the PP group (26 days) was based on subsets of only 4 and 3 patients, respectively. We did not see any relapses in the 12 patients who achieved remission at later than 141 days of prednisolone treatment, most likely because of the short follow-up period of 365 days. It is therefore hard to judge whether the difference in duration of the remission between the groups is a trend or merely a coincidence.A positive trend, which was statistically not significant, was the lower mean prednisolone dose used to achieve remission in the DP group. We should remember, however, that in this trial a very quick tapering of the prednisolone dose to 0 mg within 19 weeks was chosen to detect early relapses in the control group. The positive trends in the DP group might have been leveled if prednisolone treatment had not been tapered to less than 10 mg/d in both groups, as is often used in practice. However, relapses occurring in this quick tapering schedule could be controlled with stepped-up doses of 20 or 40 mg of prednisolone.The results of this randomized controlled trial cannot directly be compared with those of other open patient series in which DP was used in combination with cyclophosphamide, usually called dexamethasone-cyclophosphamide pulse (DCP) therapy.In DCP therapy, patients receive low daily doses of oral prednisolone and cyclophosphamide, 50 mg/d.We chose azathioprine instead of cyclophosphamide as the CS-sparing agent because the latter has the potential to induce malignanciesand might result in permanent sterilization in women. The surprisingly good results of the PP (control) group in this study might be attributed to the continued azathioprine administration during the study at a higher dosage (3 mg/kg per day) than is commonly used (<2 mg/kg per day). Randomized placebo-controlled studies addressing the effect of azathioprine in pemphigus are lacking, although its CS-sparing effect has been shown in other autoimmune bullous diseases.A second difference compared with the DCP study is that we stopped pulses after tapering prednisolone treatment to 0 mg, whereas DCP therapy was continued for 6 months. A third difference is that we used oral instead of intravenous CS pulses. Oral pulses are more convenient and serve as a more acceptable blinding method for placebo. Oral dexamethasone pulsing has a bioavailability of 60%.In this study, only patients with newly diagnosed pemphigus vulgaris or those in complete remission with a new flare-up were included, so the results do not extend to patients with refractory disease that is resistant to conventional therapy. Adjuvant pulse therapy may have a value for severe or refractory cases of pemphigus.We saw a significantly larger weight gain in patients in the DP group. This weight gain was probably due to cushingoid obesity, which is a known to be an adverse effect of CS therapy. In this study, a small number of adverse events were probably caused by azathioprine, such as liver and kidney dysfunction, anemia, and leukopenia. These effects were reversible after lowering the azathioprine dose. The end-of-study dual-energy x-ray absorptiometry scans showed no signs of osteoporosis in any patient.With regard to the anti-Dsg1 and anti-Dsg3 ELISA results, both study groups showed a significant decline between the start and remission levels of Dsg1 antibodies, and also, but to a lesser extent, in Dsg3 antibodies. After discontinuation of CS therapy, a slight increase in Dsg3 antibody levels was seen in both groups, but in Dsg1 antibody levels only in the PP group. We could not find a statistically significant difference in reduction of antibody levels between the groups.As we stated, the conclusions that can be drawn from this study for the treatment of patients with pemphigus vulgaris are somewhat restricted because of the small number of patients (n = 20), the relatively short follow-up period (365 days), and the restriction to patients with new disease activity. However, we believe that when the observed treatment differences are considered from a clinical respective, these restrictions do not alter our overall recommendation against using DP therapy in this patient group. The results from both groups imply that conventional treatment with prednisolone, 80 mg/d, on a tapering schedule to 0 mg in 19 weeks, and azathioprine sodium, 3 mg/kg, continued for 1 year after tapering, is an effective therapy for new disease activity in patients with pemphigus vulgaris, and that adding DP therapy to this regimen has no benefit.Correspondence:Marcel F. Jonkman, MD, PhD, Department of Dermatology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, the Netherlands (m.f.jonkman@derm.umcg.nl).Accepted for Publication:January 19, 2006.Author Affiliations:Center for Blistering Diseases, Department of Dermatology (Drs Mentink, Mackenzie, Tóth, and Jonkman), Department of Pharmacy (Dr Laseur), and Trial Coordinations Center (Messrs Lambert and Veeger), University Medical Center Groningen, University of Groningen, Groningen, the Netherlands; Department of Immunodermatology, Instituto Dermopatico dell’Immacolata-IRCCS, Rome, Italy (Dr Cianchini); and Department of Dermatology, Military Medical Academy, Belgrade, Serbia, and Montenegro (Dr Pavlovi&cacute;). Dr Mackenzie is now with the Department of Dermatology, University Medical Center St Radboud, Nijmegen, the Netherlands; Dr Tóth, Department of Dermatology, Martini Hospital, Groningen; and Mr Lambert, PharmaBio-Research, Groningen.Author Contributions:The authors had full access to the data and were the sole preparers of this manuscript. Study concept and design: Mentink, Tóth, Laseur, Lambert, and Jonkman. Acquisition of data: Mentink, Mackenzie, Tóth, Lambert, Cianchini, and Pavlovi&cacute;. Analysis and interpretation of data: Mentink and Veeger. Drafting of the manuscript: Mentink and Jonkman. Critical revision of the manuscript for important intellectual content: Mentink, Veeger, and Jonkman. Statistical analysis: Veeger. Obtained funding: Tóth and Jonkman. Administrative, technical, and material support: Mentink, Mackenzie, Tóth, Laseur, Lambert, and Pavlovi&cacute;. Study supervision and drafting of revised manuscript: Jonkman.Financial Disclosure:None.Funding/Support:This study was supported by a research grant from the University Medical Center Groningen, Groningen, the Netherlands.Previous Presentations:This study was presented at the 35th Annual Meeting of the European Sociey for Dermatological Research; September 22, 2005; Tübingen, Germany; and at the Annual Meeting of the Medical Dermatology Society; March 2, 2006; San Francisco, Calif.Additional Information:This manuscript was not prepared by someone other than the authors.Acknowledgment:We thank the following colleagues for voluntarily recruiting and treating patients for this study: Sandor Husz, MD (Hungary), Koos Sanders, MD (the Netherlands), Pilar Iranzo, MD (Spain), Hilde Beele, MD, and Barbara Boone, MD (Belgium); for data entry: Marco Assmann, MSc; for thiopurine methyltransferase analysis: Ido Kema, PhD; for pharmaceutical support: Ida Sikkema, RPh; for desmogleins ELISA: Hendri Pas, PhD; and for linguistic correction of the manuscript: Dédée Murrell, MD (Sydney, New South Wales).This article was corrected on 5/23/06, prior to publication of the correction in print.REFERENCESJCBystrynJLRudolphPemphigus.Lancet2005366617315993235KEHarmanSAlbertMMBlackGuidelines for the management of pemphigus vulgaris.Br J Dermatol200314992693714632796JSPasrichaBKKhaitanRSRamanMChandraDexamethasone-cyclophosphamide pulse therapy for pemphigus.Int J Dermatol1995348758828647673JSPasrichaJThanzamaUKKhanIntermittent high-dose dexamethasone-cyclophosphamide therapy for pemphigus.Br J Dermatol198811973773408666SKaurAJKanwarDexamethasone-cyclophosphamide pulse therapy in pemphigus.Int J Dermatol1990293713742361797JSPasrichaSSDasCurative effect of dexamethasone-cyclophosphamide pulse therapy for the treatment of pemphigus vulgaris.Int J Dermatol1992318758771478770FChryssomallisADimitriadesGCChaidemenosDPanagiotidesGKarakatsanisSteroid-pulse therapy in pemphigus vulgaris long term follow-up.Int J Dermatol1995344384427657447JSPasrichaBKKhaitanCurative treatment for pemphigus.Arch Dermatol1996132151815198961890VPWerthTreatment of pemphigus vulgaris with brief, high-dose intravenous glucocorticoids.Arch Dermatol1996132143514398961871FFemianoFGombosCScullyPemphigus vulgaris with oral involvement.J Eur Acad Dermatol Venereol20021635335612224691AJKanwarSKaurGPThamiLong-term efficacy of dexamethasone-cyclophosphamide pulse therapy in pemphigus.Dermatology200220422823112037452MDMignognaLLo MuzioERuoppoSFedeleLLo RussoEBucciHigh-dose intravenous “pulse” methylprednisone in the treatment of severe oropharyngeal pemphigus: a pilot study.J Oral Pathol Med20023133934412201245GGTothJBvan de MeerMFJonkmanDexamethasone pulse therapy in pemphigus.J Eur Acad Dermatol Venereol20021660761112482045RJainBKumarImmediate and delayed complications of dexamethasone cyclophosphamide pulse (DCP) therapy.J Dermatol20033071371814684952SSacchidanandNCHiremathHVNatrajDexamethasone-cyclophosphamide pulse therapy for autoimmune-vesiculobullous disorders at Victoria hospital, Bangalore.Dermatol Online J200392http://dermatology.cdlib.org/95/original/pemphigus/sacchidanand.html. 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JAMA DermatologyAmerican Medical Association

Published: May 1, 2006

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