Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You and Your Team.

Learn More →

Quantitative Microdetermination Of Enzymes in Sweat Gland: IV. Phosphatidic Acid Cycle in Cystic Fibrosis

Quantitative Microdetermination Of Enzymes in Sweat Gland: IV. Phosphatidic Acid Cycle in Cystic... Abstract ONE OF THE manifestations of cystic fibrosis is failure to produce a normal hypotonicity in the sweat. This defect may be due to inability to retrieve sodium and chloride from a precursor fluid. It may thus be failure of a "sodium pump" mechanism for secreting sodium from the sweat gland lumen into the extracellular fluid. Such a sodium pump mechanism has been investigated in the "salt gland" of the albatross and other marine birds.1 It has also been investigated in the electrolyte transport across erythrocyte membranes.2 The present study was undertaken to test the possibility that failure of this mechanism was involved in the defect of the sweat glands in cystic fibrosis. The mechanism in the avian salt glands and the erythrocyte membrane has been shown to be accompanied by a turnover of phospholipids as indicated by incorporation of radioactive phosphate, particularly into phosphatidic acid. This phosphatidic acid References 1. Hokin, L. E., and Hokin, M. R.: Studies on Carrier Function of Phosphatidic Acid in Sodium Transport , J Gen Physiol 44:61-85, 1960.Crossref 2. Hokin, L. E., and Hokin, M. R.: Diglyceride Kinase and Phosphatidic Acid Phosphatase in Erythrocyte Membranes , Nature 189:836-837, 1961.Crossref 3. Hokin, L. E.; Hokin, M. R.; and Lobeck, C. C.: Effects of Acetylcholine on Incorporation of P32 Into Phospholipids in Slices of Skin From Children With and Without Cystic Fibrosis of Pancreas , J Clin Invest 42:1232-1237, 1963.Crossref 4. Salk, J. E.; Youngner, J. S.; and Ward, E. N.: Use of Color Change of Phenol Red as Indicator in Titrating Poliomyelitis Virus or Its Antibody in Tissue-Culture System , Amer J Hyg 60:214, 1954. 5. Gibbs, G. E., and Reimer, K.: Quantitative Microdetermination of Enzymes in Sweat Gland: II. Dehydrogenases in Patients With Cystic Fibrosis and in Control Subjects , J Pediat 65:540-541, 1964.Crossref 6. Hack, M. H.: Chromatography of Phosphatides on Silicic Acid Impregnated Filter Paper , J Chromatog ( (5) ):531:540, 1961. 1961. 7. Kennedy, E. P.: Phosphatidic Acid Phosphatase, Methods in Enzymology , Colowick-Kaplan, New York: Academic Press, 1962, vol 5, p 482. 8. Kates, M.: Hydrolysis of Lecithin by Plant Plastid Enzymes , Canad J Biochem 33:575, 1955.Crossref 9. Kates, M.: Lecithinase Systems in Sugar Beet, Spinach, Cabbage, and Carrot , Canad J Biochem Physiol 32:571-573, 1954. 1954.Crossref 10. Gomori, G.: Modification of Colorimetric Phosphorous Determination for Use With Phostoelectric Colorimeter , J Lab Clin Med 27:955, 1942. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Diseases of Children American Medical Association

Quantitative Microdetermination Of Enzymes in Sweat Gland: IV. Phosphatidic Acid Cycle in Cystic Fibrosis

Loading next page...
 
/lp/american-medical-association/quantitative-microdetermination-of-enzymes-in-sweat-gland-iv-BV0cac7dPd
Publisher
American Medical Association
Copyright
Copyright © 1965 American Medical Association. All Rights Reserved.
ISSN
0002-922X
DOI
10.1001/archpedi.1965.02090030087012
Publisher site
See Article on Publisher Site

Abstract

Abstract ONE OF THE manifestations of cystic fibrosis is failure to produce a normal hypotonicity in the sweat. This defect may be due to inability to retrieve sodium and chloride from a precursor fluid. It may thus be failure of a "sodium pump" mechanism for secreting sodium from the sweat gland lumen into the extracellular fluid. Such a sodium pump mechanism has been investigated in the "salt gland" of the albatross and other marine birds.1 It has also been investigated in the electrolyte transport across erythrocyte membranes.2 The present study was undertaken to test the possibility that failure of this mechanism was involved in the defect of the sweat glands in cystic fibrosis. The mechanism in the avian salt glands and the erythrocyte membrane has been shown to be accompanied by a turnover of phospholipids as indicated by incorporation of radioactive phosphate, particularly into phosphatidic acid. This phosphatidic acid References 1. Hokin, L. E., and Hokin, M. R.: Studies on Carrier Function of Phosphatidic Acid in Sodium Transport , J Gen Physiol 44:61-85, 1960.Crossref 2. Hokin, L. E., and Hokin, M. R.: Diglyceride Kinase and Phosphatidic Acid Phosphatase in Erythrocyte Membranes , Nature 189:836-837, 1961.Crossref 3. Hokin, L. E.; Hokin, M. R.; and Lobeck, C. C.: Effects of Acetylcholine on Incorporation of P32 Into Phospholipids in Slices of Skin From Children With and Without Cystic Fibrosis of Pancreas , J Clin Invest 42:1232-1237, 1963.Crossref 4. Salk, J. E.; Youngner, J. S.; and Ward, E. N.: Use of Color Change of Phenol Red as Indicator in Titrating Poliomyelitis Virus or Its Antibody in Tissue-Culture System , Amer J Hyg 60:214, 1954. 5. Gibbs, G. E., and Reimer, K.: Quantitative Microdetermination of Enzymes in Sweat Gland: II. Dehydrogenases in Patients With Cystic Fibrosis and in Control Subjects , J Pediat 65:540-541, 1964.Crossref 6. Hack, M. H.: Chromatography of Phosphatides on Silicic Acid Impregnated Filter Paper , J Chromatog ( (5) ):531:540, 1961. 1961. 7. Kennedy, E. P.: Phosphatidic Acid Phosphatase, Methods in Enzymology , Colowick-Kaplan, New York: Academic Press, 1962, vol 5, p 482. 8. Kates, M.: Hydrolysis of Lecithin by Plant Plastid Enzymes , Canad J Biochem 33:575, 1955.Crossref 9. Kates, M.: Lecithinase Systems in Sugar Beet, Spinach, Cabbage, and Carrot , Canad J Biochem Physiol 32:571-573, 1954. 1954.Crossref 10. Gomori, G.: Modification of Colorimetric Phosphorous Determination for Use With Phostoelectric Colorimeter , J Lab Clin Med 27:955, 1942.

Journal

American Journal of Diseases of ChildrenAmerican Medical Association

Published: Jul 1, 1965

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$499/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month