Abstract ONE OF THE manifestations of cystic fibrosis is failure to produce a normal hypotonicity in the sweat. This defect may be due to inability to retrieve sodium and chloride from a precursor fluid. It may thus be failure of a "sodium pump" mechanism for secreting sodium from the sweat gland lumen into the extracellular fluid. Such a sodium pump mechanism has been investigated in the "salt gland" of the albatross and other marine birds.1 It has also been investigated in the electrolyte transport across erythrocyte membranes.2 The present study was undertaken to test the possibility that failure of this mechanism was involved in the defect of the sweat glands in cystic fibrosis. The mechanism in the avian salt glands and the erythrocyte membrane has been shown to be accompanied by a turnover of phospholipids as indicated by incorporation of radioactive phosphate, particularly into phosphatidic acid. This phosphatidic acid References 1. Hokin, L. E., and Hokin, M. R.: Studies on Carrier Function of Phosphatidic Acid in Sodium Transport , J Gen Physiol 44:61-85, 1960.Crossref 2. Hokin, L. E., and Hokin, M. R.: Diglyceride Kinase and Phosphatidic Acid Phosphatase in Erythrocyte Membranes , Nature 189:836-837, 1961.Crossref 3. Hokin, L. E.; Hokin, M. R.; and Lobeck, C. C.: Effects of Acetylcholine on Incorporation of P32 Into Phospholipids in Slices of Skin From Children With and Without Cystic Fibrosis of Pancreas , J Clin Invest 42:1232-1237, 1963.Crossref 4. Salk, J. E.; Youngner, J. S.; and Ward, E. N.: Use of Color Change of Phenol Red as Indicator in Titrating Poliomyelitis Virus or Its Antibody in Tissue-Culture System , Amer J Hyg 60:214, 1954. 5. Gibbs, G. E., and Reimer, K.: Quantitative Microdetermination of Enzymes in Sweat Gland: II. Dehydrogenases in Patients With Cystic Fibrosis and in Control Subjects , J Pediat 65:540-541, 1964.Crossref 6. Hack, M. H.: Chromatography of Phosphatides on Silicic Acid Impregnated Filter Paper , J Chromatog ( (5) ):531:540, 1961. 1961. 7. Kennedy, E. P.: Phosphatidic Acid Phosphatase, Methods in Enzymology , Colowick-Kaplan, New York: Academic Press, 1962, vol 5, p 482. 8. Kates, M.: Hydrolysis of Lecithin by Plant Plastid Enzymes , Canad J Biochem 33:575, 1955.Crossref 9. Kates, M.: Lecithinase Systems in Sugar Beet, Spinach, Cabbage, and Carrot , Canad J Biochem Physiol 32:571-573, 1954. 1954.Crossref 10. Gomori, G.: Modification of Colorimetric Phosphorous Determination for Use With Phostoelectric Colorimeter , J Lab Clin Med 27:955, 1942.
American Journal of Diseases of Children – American Medical Association
Published: Jul 1, 1965
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