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Quantitation of Inflammation in the Cornea

Quantitation of Inflammation in the Cornea Abstract Corneal inflammation, produced by direct interlamellar injection of clove oil, is histologically characterized primarily by polymorphonuclear leukocytic infiltration of the stroma. The polymorphonuclear leukocytes can be radiolabelled with tritiated thymidine systemically prior to their invasion of the cornea. A measure of the number of labelled polymorphonuclear leukocytes in the cornea can then be obtained by scintillation counting techniques, which provides an objective method for quantitating the degree of inflammation in the cornea. References 1. Cox WV, Kupferman A, Leibowitz HM: Topically applied steroids in corneal disease: I. The role of inflammation in stromal absorption of dexamethasone . Arch Ophthalmol 88:308-313, 1972.Crossref 2. Cox WV, Kupferman A, Leibowitz HM: Topically applied steroids in corneal disease: II. The role of drug vehicle in stromal absorption of dexamethasone . Arch Ophthalmol 88:549-552, 1972.Crossref 3. Kupferman A, et al: Topically applied steroids in corneal disease: III. The role of drug derivative in stromal absorption of dexamethasone . Arch Ophthalmol 91:373-376, 1974.Crossref 4. Kupferman A, Leibowitz HM: Topically applied steroids in corneal disease: IV. The role of drug concentration in stromal absorption of prednisolone acetate . Arch Ophthalmol 91:377-380, 1974.Crossref 5. Kupferman A, Leibowitz HM: Topically applied steroids in corneal disease: V. Dexamethasone alcohol . Arch Ophthalmol 92:329-330, 1974.Crossref 6. Kupferman A, Leibowitz HM: Topically applied steroids in corneal disease: VI. Kinetics of prednisolone phosphate . Arch Ophthalmol 92:331-334, 1974.Crossref 7. Sugar J, et al: Tetrahydrotriamcinolone and triamcinolone . Invest Ophthalmol 11:890-893, 1972. 8. Robb RH, Kuwabara T: Corneal wound healing: II. Autoradiographic study of the cellular components . Arch Ophthalmol 72:401-408, 1964.Crossref 9. Snedecor GW: Statistical Methods Applied to Experiments in Agriculture and Biology , ed 5. Ames, Iowa, Iowa State College, 1956. 10. Hanna C, O'Brien JE: Cell production and migration in the epithelial layer of the cornea . Arch Ophthalmol 64:536-539, 1960.Crossref 11. Kopriwa BM, Leblowd CP: Improvements in the coating technique of radioautography . J Histochem Cytochem 10:269-284, 1962.Crossref 12. Aronson SB, Elliott JH: Ocular Inflammation . St. Louis, C V Mosby Co, 1972. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Quantitation of Inflammation in the Cornea

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Publisher
American Medical Association
Copyright
Copyright © 1974 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1974.01010010439014
Publisher site
See Article on Publisher Site

Abstract

Abstract Corneal inflammation, produced by direct interlamellar injection of clove oil, is histologically characterized primarily by polymorphonuclear leukocytic infiltration of the stroma. The polymorphonuclear leukocytes can be radiolabelled with tritiated thymidine systemically prior to their invasion of the cornea. A measure of the number of labelled polymorphonuclear leukocytes in the cornea can then be obtained by scintillation counting techniques, which provides an objective method for quantitating the degree of inflammation in the cornea. References 1. Cox WV, Kupferman A, Leibowitz HM: Topically applied steroids in corneal disease: I. The role of inflammation in stromal absorption of dexamethasone . Arch Ophthalmol 88:308-313, 1972.Crossref 2. Cox WV, Kupferman A, Leibowitz HM: Topically applied steroids in corneal disease: II. The role of drug vehicle in stromal absorption of dexamethasone . Arch Ophthalmol 88:549-552, 1972.Crossref 3. Kupferman A, et al: Topically applied steroids in corneal disease: III. The role of drug derivative in stromal absorption of dexamethasone . Arch Ophthalmol 91:373-376, 1974.Crossref 4. Kupferman A, Leibowitz HM: Topically applied steroids in corneal disease: IV. The role of drug concentration in stromal absorption of prednisolone acetate . Arch Ophthalmol 91:377-380, 1974.Crossref 5. Kupferman A, Leibowitz HM: Topically applied steroids in corneal disease: V. Dexamethasone alcohol . Arch Ophthalmol 92:329-330, 1974.Crossref 6. Kupferman A, Leibowitz HM: Topically applied steroids in corneal disease: VI. Kinetics of prednisolone phosphate . Arch Ophthalmol 92:331-334, 1974.Crossref 7. Sugar J, et al: Tetrahydrotriamcinolone and triamcinolone . Invest Ophthalmol 11:890-893, 1972. 8. Robb RH, Kuwabara T: Corneal wound healing: II. Autoradiographic study of the cellular components . Arch Ophthalmol 72:401-408, 1964.Crossref 9. Snedecor GW: Statistical Methods Applied to Experiments in Agriculture and Biology , ed 5. Ames, Iowa, Iowa State College, 1956. 10. Hanna C, O'Brien JE: Cell production and migration in the epithelial layer of the cornea . Arch Ophthalmol 64:536-539, 1960.Crossref 11. Kopriwa BM, Leblowd CP: Improvements in the coating technique of radioautography . J Histochem Cytochem 10:269-284, 1962.Crossref 12. Aronson SB, Elliott JH: Ocular Inflammation . St. Louis, C V Mosby Co, 1972.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Nov 1, 1974

References

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