Abstract • Rabbits undergoing full-thickness glaucoma filtering surgery were exposed for 5 minutes to one of three intraoperative treatments: (1) distilled water; (2) fluorouracil, 50 mg/mL; or (3) mitomycin C, 0.4 mg/mL. Tissue samples were taken from the subconjunctival and scleral tissues at the treated area and 90° and 180° from the center of the treated area and the adjacent cornea 2 mm from the limbus, 1 hour, 5 days, and 30 days postoperatively. The biopsy specimens were then placed in tissue culture media and the fibroblast outgrowths measured. Five-minute intraoperative treatments with fluorouracil resulted in a reversible delay of fibroblast outgrowths from treated subconjunctival and scleral tissues of just over 1 week in this model, whereas treatment with mitomycin C, 0.4 mg/mL, resulted in prolonged inhibition of at least 30 days. These effects were localized to the area treated. The many clinical implications of these findings are discussed. References 1. 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The effects of subconjunctival mitomycin-c on glaucoma filtration surgery in rabbits . Arch Ophthalmol . 1991;109:1725-1730.Crossref 8. Pasquale LR,Thibault D, Dorman-Pease ME, Quigley HA, Jampel HD. Effect of topical mitomycin C on glaucoma filtration surgery in monkeys . Ophthalmology . 1992;99:14-18.Crossref 9. Chen CW, Huang HT, Bair JS, Lee CC. Trabeculectomy with simultaneous topical application of mitomycin-C in refractory glaucoma . J Ocul Pharmacol . 1990;6:175-182.Crossref 10. Palmer SS. Mitomycin as an adjunct chemotherapy with trabeculectomy . Ophthalmology . 1991;98:317-321.Crossref 11. Kitazawa Y, Kawase K, Matsushita H, Minobe M. Trabeculectomy with mitomycin: a comparative study with fluorouracil . Arch Ophthalmol . 1991;109:1693-1698.Crossref 12. Skuta GL, Beeson CC, Higginbotham EJ, et al. Intraoperative mitomycin versus postoperative 5-fluorouracil in high-risk glaucoma filtering surgery . Ophthalmology . 1992;99:438-444.Crossref 13. Kawase K, Matsushita H, Yamamoto T, Kitazawa Y. Mitomycin concentration in rabbit and human ocular tissues after topical administration . Ophthalmology . 1992;99:203-207.Crossref 14. Khaw PT, Sherwood MB, MacKay SLD, Rossi MB, Schultz GS. 5-Minute treatments with fluorouracil, floxuridine and mitomycin have long-term effects on human Tenon's capsule fibroblasts . Arch Ophthalmol . 1992;110:1150-1154.Crossref 15. Khaw PT, Doyle JW, Sherwood MB, Smith MF, McGorray S. Effects of intraoperative 5-fluorouracil or mitomycin-c on glaucoma filtration surgery in the rabbit. Ophthalmology. In press. 16. Seetner A, Morin JD. Healing of trabeculectomies in rabbits . Can J Ophthalmol . 1979;14:121-125. 17. Miller MH, Grierson I, Unger WI, Hitchings RA. Wound healing in an animal model of glaucoma fistulizing surgery in the rabbit . Ophthalmic Surg . 1989;20:350-357. 18. Khaw PT, Ward S, Porter A, Grierson I, Hitchings RA, Rice NSC. The long term effects of 5-fluorouracil and sodium butyrate on human Tenon's capsule fibroblasts . Invest Ophthalmol Vis Sci . 1992;33:2043-2052. 19. Yamamoto T, Varani J, Kaz Soong H, Lichter PR. Effect of 5-fluorouracil and mitomycin-c on cultured rabbit subconjunctival fibroblasts . Ophthalmology . 1990;97:1204-1210.Crossref 20. Khaw PT, Ward S, Grierson I, Rice NSC. The effects of beta-radiation on proliferating human Tenon's capsule fibroblasts . Br J Ophthalmol . 1991;75:580-583.Crossref 21. Minckler DS, Shammas A, Wilcox M, Ogden TE. Experimental studies of aqueous filtration using the Molteno implant . Trans Am Ophthalmol Soc . 1987;85:368-392. 22. Heuer DK, Lloyd MA, Abrams DA, et al. Preliminary report of a randomised clinical trial of single plate versus double plate Molteno implantation for glaucomas in aphakia and pseudophakia . In: Kriegelstein GK, ed. Glaucoma Update IV . New York, NY: Springer-Verlag NY Inc; 1991:244-249. 23. Wilson RP, Cantor L, Katz LJ, Schmidt CM, Steinmann WC, Allee S. Aqueous shunts: Molteno vs Schocket . Ophthalmology . 1992;99:672-678.Crossref 24. Nordlund JR, Pasquale LR, Quigley HA, Jampel HD. Effectiveness of intraoperative 5-fluorouracil versus intraoperative mitomycin-c in experimental rabbit glaucoma surgery . Invest Ophthalmol Vis Sci . 1992;33( (suppl) ):1393.
Archives of Ophthalmology – American Medical Association
Published: Feb 1, 1993
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