Abstract • The penetration of systemically administered fluorescein sodium into the eye depends on several factors. One important variable is the unbound plasma fluorescein concentration. We measured the in vitro plasma binding of fluorescein in 200 persons using fluorescence polarization. We demonstrated considerable variability in the degree of plasma binding and also found significant differences between normal persons and diabetic and nondiabetic patients. References 1. Goldmann H: Über Fluorescein in der menschlichen Vorderkammer: Das Kammerwasser-Minulenvolumen des Menschen . Ophthalmologica 1950;119:65-95.Crossref 2. Andersson L.-O, Rehnstrom A, Eaker DL: Studies on nonspecific binding: The nature of the binding of fluorescein to bovine-serum albumin . Eur J Biochem 1971;20:371-380.Crossref 3. Nagataki S: Aqueous humor dynamics of human eyes as studied using fluorescein . Jpn J Ophthalmol 1975;19:235-249. 4. Brubaker RF, Penniston JT, Grotte DA, et al: Measurement of fluorescein binding in human plasma using fluorescence polarization . Arch Ophthalmol 1982;100:625-630.Crossref 5. Penniston JT: Fluorescence polarization measurement of binding of fluorescein to albumin. Exp Eye Res, in press. 6. Araie M, Sawa M, Nagataki S, et al: Aqueous humor dynamics in man as studied by oral fluorescein. Jpn J Ophthalmol, in press.
Archives of Ophthalmology – American Medical Association
Published: Jul 1, 1982