Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You and Your Team.

Learn More →

Pathologic Human Vitreous Promotes Contraction by Fibroblasts: Implications for Proliferative Vitreoretinopathy

Pathologic Human Vitreous Promotes Contraction by Fibroblasts: Implications for Proliferative... Abstract Objectives: To establish and quantify the presence of contraction-stimulating activity in pathologic vitreous and correlate this activity with clinical presentation and outcome, especially with proliferative vitreoretinopathy. Methods: Contraction-stimulating activity of vitreous collected during surgery was quantified with a tissue culture assay using fibroblasts as target cells. The activity of each sample was correlated with patient history, clinical presentation, risk factors, proliferative disease, and postoperative proliferation. Results: Pathologic vitreous contained measurable quantities of contraction-stimulating activity and stimulated contraction in vitro, with elevated activities in samples from patients with proliferative vitreoretinopathy, epimacular proliferation, retinal detachment, retinal defects, pigmented cells in the vitreous, hemorrhage, or uveitis. Patients with postoperative proliferation had significantly elevated mean activities. Conclusions: Levels of contraction-stimulating activity in pathologic vitreous correlate with some risk factors for the development of proliferative vitreoretinopathy and may ultimately be useful in the assessment of disease severity and the prediction of postoperative proliferation. References 1. Hanneken A, Michels R. Surgical treatment of PVR . In: Freeman H, Toletino F, eds. Proliferative Vitreoretinopathy (PVR) . New York, NY: Springer-Verlag NY Inc; 1988:60-69. 2. Baudouin C, Fredj-Reygrobellet D, Brignole F, Negre F, Lapalus P, Gastaud P. Growth factors in vitreous and subretinal fluid cells from patients with proliferative vitreoretinopathy . Ophthalmic Res . 1993;25:52-59.Crossref 3. McCuen B, Landers M, Machemer R. The use of silicone oil following failed vitrectomy for retinal detachment with advanced proliferative vitreoretinopathy . Ophthalmology . 1985;92:1029-1033.Crossref 4. Machemer R. Proliferative vitreoretinopathy (PVR): a personal account of its pathogenesis and treatment . Invest Ophthalmol Vis Sci . 1988;29:1771-1783. 5. Weller M, Wiedemann P, Heimann K. Proliferative vitreoretinopathy: is it anything more than wound healing at the wrong place? Int Ophthalmol . 1990;14:105-117.Crossref 6. Cowley M, Conway B, Campochiaro P, Kaiser D, Gaskin H. Clinical risk factors for proliferative vitreoretinopathy . Arch Ophthalmol . 1989;107:1147-1151.Crossref 7. Hutton W, Fuller D, Snyder W. Silicone oil for management of PVR: comparison of six-month and two-year results . In: Freeman H, Toletino F, eds. Proliferative Vitreoretinopathy (PVR) . New York, NY: Springer-Verlag NY Inc; 1988:166-168. 8. Lewis H, Aaberg T, Abrams G. Causes of failure after initial vitreoretinal surgery for severe proliferative vitreoretinopathy . Am J Ophthalmol . 1991;111:8-14. 9. Machemer R, Laqua H. Pigment epithelium proliferation in retinal detachment (massive periretinal proliferation) . Am J Ophthalmol . 1975;80:1-23. 10. Weller M, Heimann K, Wiedemann P. The pathogenesis of vitreoretinal proliferation and traction: a working hypothesis . Med Hypotheses . 1990;31:157-159.Crossref 11. Cousins S, Rubsamen P. Comparison of flow cytometry with the surgeon regarding ability to predict the ultimate success of surgery for proliferative vitreoretinopathy . Arch Ophthalmol . 1994;112:1554-1560.Crossref 12. Wiedemann P, Weller M. The pathophysiology of proliferative vitreoretinopathy . Acta Ophthalmologica (Copenh) . 1988;189:4-15. 13. Bell E, Ivarsson B, Merril C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro . Proc Natl Acad Sci U S A . 1979;76:1274-1278.Crossref 14. Steinberg B, Smith K, Colozzo M, Pollack R. Establishment and transformation diminish the ability of fibroblasts to contract a native collagen gel . J Cell Biol . 1980;87:304-308.Crossref 15. Guidry C, Grinnell F. Contraction of hydrated collagen gels by fibroblasts: evidence for two mechanisms through which fibrils are stabilized . Coll Related Res . 1986;6:515-529.Crossref 16. Guidry C, Grinnell F. Studies on the mechanism of hydrated collagen gel reorganization by human skin fibroblasts . J Cell Sci . 1985;79:67-81. 17. Gullberg D, Tingstrom A, Thuresson A-C, et al. Beta-1 integrin-mediated collagen gel contraction is stimulated by PDGF . Exp Cell Res . 1990;186:264-272.Crossref 18. Klein C, Dressel D, Steinmayer T, et al. Integrin alpha 2 beta 1 is upregulated in fibroblasts and highly aggressive melanoma cells in three-dimensional collagen lattices and mediates the reorganization of collagen I fibrils . J Cell Biol . 1991;115:1427-1436.Crossref 19. Schiro J, Chan B, Roswit W, et al. Integrin α2 β1 (VLA-2) mediates reorganization and contraction of collagen matrices by human cells . Cell . 1991;67:403-410.Crossref 20. Kupper T, Ferguson T. A potential pathophysiologic role for α2 β1 integrin in human eye diseases involving vitreoretinal traction . FASEB J . 1993;7:1401-1406. 21. Hunt R, Pakalnis V, Choudhury P, Black P. Cytokines and serum cause α2 β1 integrin-mediated contraction of collagen gels by cultured retinal pigment epithelial cells . Invest Ophthalmol Vis Sci . 1994;35:955-963. 22. Glaser B, Cardin A, Biscoe B. Proliferative vitreoretinopathy: the mechanism of development of vitreoretinal detachment . Ophthalmology . 1987;94:327-332.Crossref 23. Yamakawa R, Sorgente N, Ryan S. Investigations on contractile properties of retinal pigment epithelial cells . Ophthalmologica . 1989;199:165-172.Crossref 24. Raymond M, Thompson J. RPE-mediated collagen gel contraction: inhibition by colchicine and stimulation by TGF-α . Invest Ophthalmol Vis Sci . 1990;31:1070-1086. 25. Guidry C, McFarland R, Morris R, Witherspoon C, Hook M. Collagen gel contraction by cells associated with proliferative vitreoretinopathy . Invest Ophthalmol Vis Sci . 1992;33:85-91. 26. Mazure A, Grierson I. In vitro studies of the contractility of cell types involved in proliferative vitreoretinopathy . Invest Ophthalmol Vis Sci . 1992;33:3407-3416. 27. Grisanti S, Guidry C. Transdifferentiation of retinal pigment epithelial cells from epithelial to mesenchymal phenotype . Invest Ophthalmol Vis Sci . 1995;35:391-405. 28. Finesmith T, Broadley K, Davidson J. Fibroblasts from wounds of different stages of repair vary in their ability to contract a collagen gel in response to growth factors . J Cell Physiol . 1990;144:99-107.Crossref 29. Guidry C, Hardwick C. Extracellular matrix contraction by choroidal fibroblasts: Inhibition by staurosporine . Invest Ophthalmol Vis Sci . 1994;35:50-508. 30. de Juan E, Dickson J, Hatchell D. Interaction of retinal glial cells with collagen matrices: implications for pathogenesis of cell-mediated traction . Graefes Arch Clin Exp Ophthalmol . 1989;227:494-498.Crossref 31. Guidry C, Hohn S, Hook M. Endothelial cells secrete a factor that promotes fibroblast contraction of hydrated collagen gels . J Cell Biol . 1990;110:519-528.Crossref 32. Montesano R. Orci L. Transforming growth factor β stimulates collagen-matrix contraction by fibroblasts: implication for wound healing . Proc Natl Acad Sci US A . 1988;85:4894-4897.Crossref 33. Ura H, Obara T, Yokota K, Shibata Y, Okamura K, Namiki M. Effects of transforming growth factor-β released from gastric carcinoma cells on the contraction of collagen-matrix gels containing fibroblasts . Cancer Res . 1991;51:3550-3554. 34. Pena R, Jerdan J, Glaser B. Effects of TGF-β and TGF-β neutralizing antibodies on fibroblast-induced collagen gel contraction: implications for proliferative vitreoretinopathy . Invest Ophthalmol Vis Sci . 1994;35:2804-2808. 35. Anderson S, Reben Z, Fuller G. Cell-mediated contraction of collagen lattices in serum-free medium: effect of serum and nonserum factors . In Vitro Cell Dev Biol . 1990;26:61-66.Crossref 36. Clark R, Folkvord J, Hart C, Murray M, McPherson J. Platelet isoforms of platelet-derived growth factor stimulate fibroblasts to contract collagen matrices . J Clin Invest . 1989;84:1036-1040.Crossref 37. Guidry C, Hook M. Endothelins produced by endothelial cells promote collagen gel contraction by fibroblasts . J Cell Biol . 1991;115:873-880.Crossref 38. Wiedemann P. Growth factors in retinal diseases: proliferative vitreoretinopathy. proliferative diabetic retinopathy, and retinal degeneration . Surv Ophthalmol . 1992;36:373-384.Crossref 39. Connor T, Roberts A, Sporn M, et al. Correlation of fibrosis and transforming growth factor β type 2 levels in the eye . J Clin Invest . 1989;83:1661-1666.Crossref 40. Chakravarthy U, Douglas A, Bailie J, McKibben B, Archer D. Immunoreactive endothelin distribution in ocular tissues . Invest Ophthalmol Vis Sci . 1994;35:2448-2454. 41. Liggett P, Deuel T, Freeman W, Rao N, Ryan S. Platelet-derived growth factor in choroidal melanoma . Invest Ophthalmol Vis Sci . 1986;27:132. Abstract. 42. Machemer, R, Aaberg T, Freeman H, et al. An updated classification of retinal detachment with proliferative vitreoretinopathy . Am J Ophthalmol . 1991;112:159-165. 43. Bonnet M. Clinical factors predisposing to massive proliferative vitreoretinopathy in rhegmatogenous retinal detachment . Ophthalmologica . 1984;188:148-152.Crossref 44. Bonnet M. The development of severe proliferative vitreoretinopathy after retinal detachment surgery: grade B, a determining risk factor . Graefes Arch Clin Exp Ophthalmol . 1988;226:201-205.Crossref 45. Brown B, Hollander M. Statistics: A Biomedical Introduction . New York, NY: John Wiley & Sons; 1977. 46. Lewis H. Vitreoretinal surgery and silicone oil injection for severe penetrating ocular trauma in children. Presented at the Third International Symposium on Ocular Trauma; March 10-14, 1994; Cancun, Mexico. 47. Guidry C. Extracellular matrix contraction by fibroblasts: peptide promoters and second messengers . Cancer Metastasis Rev . 1992;11:45-54.Crossref 48. Guidry C. Fibroblast contraction of collagen gels requires activation of protein kinase C . J Cell Physiol . 1993;155:358-367.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Pathologic Human Vitreous Promotes Contraction by Fibroblasts: Implications for Proliferative Vitreoretinopathy

Loading next page...
 
/lp/american-medical-association/pathologic-human-vitreous-promotes-contraction-by-fibroblasts-MVPTa5BRid
Publisher
American Medical Association
Copyright
Copyright © 1995 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1995.01100120075013
Publisher site
See Article on Publisher Site

Abstract

Abstract Objectives: To establish and quantify the presence of contraction-stimulating activity in pathologic vitreous and correlate this activity with clinical presentation and outcome, especially with proliferative vitreoretinopathy. Methods: Contraction-stimulating activity of vitreous collected during surgery was quantified with a tissue culture assay using fibroblasts as target cells. The activity of each sample was correlated with patient history, clinical presentation, risk factors, proliferative disease, and postoperative proliferation. Results: Pathologic vitreous contained measurable quantities of contraction-stimulating activity and stimulated contraction in vitro, with elevated activities in samples from patients with proliferative vitreoretinopathy, epimacular proliferation, retinal detachment, retinal defects, pigmented cells in the vitreous, hemorrhage, or uveitis. Patients with postoperative proliferation had significantly elevated mean activities. Conclusions: Levels of contraction-stimulating activity in pathologic vitreous correlate with some risk factors for the development of proliferative vitreoretinopathy and may ultimately be useful in the assessment of disease severity and the prediction of postoperative proliferation. References 1. Hanneken A, Michels R. Surgical treatment of PVR . In: Freeman H, Toletino F, eds. Proliferative Vitreoretinopathy (PVR) . New York, NY: Springer-Verlag NY Inc; 1988:60-69. 2. Baudouin C, Fredj-Reygrobellet D, Brignole F, Negre F, Lapalus P, Gastaud P. Growth factors in vitreous and subretinal fluid cells from patients with proliferative vitreoretinopathy . Ophthalmic Res . 1993;25:52-59.Crossref 3. McCuen B, Landers M, Machemer R. The use of silicone oil following failed vitrectomy for retinal detachment with advanced proliferative vitreoretinopathy . Ophthalmology . 1985;92:1029-1033.Crossref 4. Machemer R. Proliferative vitreoretinopathy (PVR): a personal account of its pathogenesis and treatment . Invest Ophthalmol Vis Sci . 1988;29:1771-1783. 5. Weller M, Wiedemann P, Heimann K. Proliferative vitreoretinopathy: is it anything more than wound healing at the wrong place? Int Ophthalmol . 1990;14:105-117.Crossref 6. Cowley M, Conway B, Campochiaro P, Kaiser D, Gaskin H. Clinical risk factors for proliferative vitreoretinopathy . Arch Ophthalmol . 1989;107:1147-1151.Crossref 7. Hutton W, Fuller D, Snyder W. Silicone oil for management of PVR: comparison of six-month and two-year results . In: Freeman H, Toletino F, eds. Proliferative Vitreoretinopathy (PVR) . New York, NY: Springer-Verlag NY Inc; 1988:166-168. 8. Lewis H, Aaberg T, Abrams G. Causes of failure after initial vitreoretinal surgery for severe proliferative vitreoretinopathy . Am J Ophthalmol . 1991;111:8-14. 9. Machemer R, Laqua H. Pigment epithelium proliferation in retinal detachment (massive periretinal proliferation) . Am J Ophthalmol . 1975;80:1-23. 10. Weller M, Heimann K, Wiedemann P. The pathogenesis of vitreoretinal proliferation and traction: a working hypothesis . Med Hypotheses . 1990;31:157-159.Crossref 11. Cousins S, Rubsamen P. Comparison of flow cytometry with the surgeon regarding ability to predict the ultimate success of surgery for proliferative vitreoretinopathy . Arch Ophthalmol . 1994;112:1554-1560.Crossref 12. Wiedemann P, Weller M. The pathophysiology of proliferative vitreoretinopathy . Acta Ophthalmologica (Copenh) . 1988;189:4-15. 13. Bell E, Ivarsson B, Merril C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro . Proc Natl Acad Sci U S A . 1979;76:1274-1278.Crossref 14. Steinberg B, Smith K, Colozzo M, Pollack R. Establishment and transformation diminish the ability of fibroblasts to contract a native collagen gel . J Cell Biol . 1980;87:304-308.Crossref 15. Guidry C, Grinnell F. Contraction of hydrated collagen gels by fibroblasts: evidence for two mechanisms through which fibrils are stabilized . Coll Related Res . 1986;6:515-529.Crossref 16. Guidry C, Grinnell F. Studies on the mechanism of hydrated collagen gel reorganization by human skin fibroblasts . J Cell Sci . 1985;79:67-81. 17. Gullberg D, Tingstrom A, Thuresson A-C, et al. Beta-1 integrin-mediated collagen gel contraction is stimulated by PDGF . Exp Cell Res . 1990;186:264-272.Crossref 18. Klein C, Dressel D, Steinmayer T, et al. Integrin alpha 2 beta 1 is upregulated in fibroblasts and highly aggressive melanoma cells in three-dimensional collagen lattices and mediates the reorganization of collagen I fibrils . J Cell Biol . 1991;115:1427-1436.Crossref 19. Schiro J, Chan B, Roswit W, et al. Integrin α2 β1 (VLA-2) mediates reorganization and contraction of collagen matrices by human cells . Cell . 1991;67:403-410.Crossref 20. Kupper T, Ferguson T. A potential pathophysiologic role for α2 β1 integrin in human eye diseases involving vitreoretinal traction . FASEB J . 1993;7:1401-1406. 21. Hunt R, Pakalnis V, Choudhury P, Black P. Cytokines and serum cause α2 β1 integrin-mediated contraction of collagen gels by cultured retinal pigment epithelial cells . Invest Ophthalmol Vis Sci . 1994;35:955-963. 22. Glaser B, Cardin A, Biscoe B. Proliferative vitreoretinopathy: the mechanism of development of vitreoretinal detachment . Ophthalmology . 1987;94:327-332.Crossref 23. Yamakawa R, Sorgente N, Ryan S. Investigations on contractile properties of retinal pigment epithelial cells . Ophthalmologica . 1989;199:165-172.Crossref 24. Raymond M, Thompson J. RPE-mediated collagen gel contraction: inhibition by colchicine and stimulation by TGF-α . Invest Ophthalmol Vis Sci . 1990;31:1070-1086. 25. Guidry C, McFarland R, Morris R, Witherspoon C, Hook M. Collagen gel contraction by cells associated with proliferative vitreoretinopathy . Invest Ophthalmol Vis Sci . 1992;33:85-91. 26. Mazure A, Grierson I. In vitro studies of the contractility of cell types involved in proliferative vitreoretinopathy . Invest Ophthalmol Vis Sci . 1992;33:3407-3416. 27. Grisanti S, Guidry C. Transdifferentiation of retinal pigment epithelial cells from epithelial to mesenchymal phenotype . Invest Ophthalmol Vis Sci . 1995;35:391-405. 28. Finesmith T, Broadley K, Davidson J. Fibroblasts from wounds of different stages of repair vary in their ability to contract a collagen gel in response to growth factors . J Cell Physiol . 1990;144:99-107.Crossref 29. Guidry C, Hardwick C. Extracellular matrix contraction by choroidal fibroblasts: Inhibition by staurosporine . Invest Ophthalmol Vis Sci . 1994;35:50-508. 30. de Juan E, Dickson J, Hatchell D. Interaction of retinal glial cells with collagen matrices: implications for pathogenesis of cell-mediated traction . Graefes Arch Clin Exp Ophthalmol . 1989;227:494-498.Crossref 31. Guidry C, Hohn S, Hook M. Endothelial cells secrete a factor that promotes fibroblast contraction of hydrated collagen gels . J Cell Biol . 1990;110:519-528.Crossref 32. Montesano R. Orci L. Transforming growth factor β stimulates collagen-matrix contraction by fibroblasts: implication for wound healing . Proc Natl Acad Sci US A . 1988;85:4894-4897.Crossref 33. Ura H, Obara T, Yokota K, Shibata Y, Okamura K, Namiki M. Effects of transforming growth factor-β released from gastric carcinoma cells on the contraction of collagen-matrix gels containing fibroblasts . Cancer Res . 1991;51:3550-3554. 34. Pena R, Jerdan J, Glaser B. Effects of TGF-β and TGF-β neutralizing antibodies on fibroblast-induced collagen gel contraction: implications for proliferative vitreoretinopathy . Invest Ophthalmol Vis Sci . 1994;35:2804-2808. 35. Anderson S, Reben Z, Fuller G. Cell-mediated contraction of collagen lattices in serum-free medium: effect of serum and nonserum factors . In Vitro Cell Dev Biol . 1990;26:61-66.Crossref 36. Clark R, Folkvord J, Hart C, Murray M, McPherson J. Platelet isoforms of platelet-derived growth factor stimulate fibroblasts to contract collagen matrices . J Clin Invest . 1989;84:1036-1040.Crossref 37. Guidry C, Hook M. Endothelins produced by endothelial cells promote collagen gel contraction by fibroblasts . J Cell Biol . 1991;115:873-880.Crossref 38. Wiedemann P. Growth factors in retinal diseases: proliferative vitreoretinopathy. proliferative diabetic retinopathy, and retinal degeneration . Surv Ophthalmol . 1992;36:373-384.Crossref 39. Connor T, Roberts A, Sporn M, et al. Correlation of fibrosis and transforming growth factor β type 2 levels in the eye . J Clin Invest . 1989;83:1661-1666.Crossref 40. Chakravarthy U, Douglas A, Bailie J, McKibben B, Archer D. Immunoreactive endothelin distribution in ocular tissues . Invest Ophthalmol Vis Sci . 1994;35:2448-2454. 41. Liggett P, Deuel T, Freeman W, Rao N, Ryan S. Platelet-derived growth factor in choroidal melanoma . Invest Ophthalmol Vis Sci . 1986;27:132. Abstract. 42. Machemer, R, Aaberg T, Freeman H, et al. An updated classification of retinal detachment with proliferative vitreoretinopathy . Am J Ophthalmol . 1991;112:159-165. 43. Bonnet M. Clinical factors predisposing to massive proliferative vitreoretinopathy in rhegmatogenous retinal detachment . Ophthalmologica . 1984;188:148-152.Crossref 44. Bonnet M. The development of severe proliferative vitreoretinopathy after retinal detachment surgery: grade B, a determining risk factor . Graefes Arch Clin Exp Ophthalmol . 1988;226:201-205.Crossref 45. Brown B, Hollander M. Statistics: A Biomedical Introduction . New York, NY: John Wiley & Sons; 1977. 46. Lewis H. Vitreoretinal surgery and silicone oil injection for severe penetrating ocular trauma in children. Presented at the Third International Symposium on Ocular Trauma; March 10-14, 1994; Cancun, Mexico. 47. Guidry C. Extracellular matrix contraction by fibroblasts: peptide promoters and second messengers . Cancer Metastasis Rev . 1992;11:45-54.Crossref 48. Guidry C. Fibroblast contraction of collagen gels requires activation of protein kinase C . J Cell Physiol . 1993;155:358-367.Crossref

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Dec 1, 1995

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$499/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month