Abstract In view of the apparent ease with which griseofulvin appears in the keratin layer of the skin after oral administration,1 an investigation of several parenteral routes of administration was carried out. Administration of varying dosages of the drug intramuscularly and subcutaneously was studied. Materials and Methods The preparation of griseofulvin employed for most of this study was a 20% suspension in peanut oil. A 20% suspension in distilled water containing 0.5% Tween 60 was also tested. For the control oral experiments, griseofulvin was administered by capsule.All of the animals employed were white male guinea pigs ranging in weight from 400 to 500 gm. Animals were infected by rubbing a heavy spore suspension of the dermatophyte onto the previously shaved and slightly abraded side of the guinea pig. As a means of indicating maintenance of griseofulvin level in the skin, infection attempts were made on alternate sides of the References 1. Infection attempts at 4, 7, 14, and 21, days post administration. t These animals were inoculated with spores four days after the medicated animals received the drug. The normal incubation period before visible signs of infection appears is three days. 2. Swartz, H. E.; Grutter, F. H.; Kamp, L. F., and Gaughran, E. R. L.: The Effect of Griseofulvin on Trichophyton Mentagrophytes Infected Guinea Pigs , Bact. Proc. 59:81, 1959. 3. Grutter, F. H.; Gaughran, E. R. L.; Swartz, H. E., and Kamp, L. F.: Microbiological Assay of Griseofulvin Using Neurospora Crassa , Bact. Proc. 59:21, 1959. 4. Swartz, H. E.: A New Assay Technique for Griseofulvin, to be published.
A.M.A. Archives of Dermatology – American Medical Association
Published: May 1, 1960