Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Paired Comparison Vasoconstrictor Assays: A Comparison of Methods for the Determination of Relative Vasoconstrictor Potency of Topically Applied Corticosteroids

Paired Comparison Vasoconstrictor Assays: A Comparison of Methods for the Determination of... Abstract The human vasoconstrictor assay, which is frequently used for potency comparisons of topically applied corticosteroids, relies on the visual measurement of skin blanching produced at the application site. In this report, three different methods of visual measurement were compared. A technique involving paired comparisons of vasoconstriction at adjacent sites was found to improve the assessment of relative vasoconstrictor activity. Corticosteroid creams containing 0.05% fluocinonide, 0.1% betamethasone valerate, and 1% hydrocortisone were evaluated in this study. References 1. McKenzie AW, Stoughton RB: Method for comparing percutaneous absorption of steroids . Arch Dermatol 86:608-610, 1962.Crossref 2. McKenzie AW: Percutaneous absorption of steroids . Arch Dermatol 86:611-614, 1962.Crossref 3. McKenzie AW, Atkinson RM: Topical activities of betamethasone esters in man . Arch Dermatol 89:741-746, 1964.Crossref 4. Stoughton RB: Bioassay system for formulations of topically applied glucocorticosteroids . Arch Dermatol 106:825-827, 1972.Crossref 5. Burdick K, et al: Corticosteroid ointments: Comparison by two human bioassays . Curr Ther Res 15:233-242, 1973. 6. Child KJ, et al: Vasoconstrictor and systemic activities of topical steroids . Arch Dermatol 97:407-410, 1968.Crossref 7. Stoughton RB: Vasoconstrictor activity and percutaneous absorption of glucocorticosteroids . Arch Dermatol 99:753-756, 1969.Crossref 8. Place VA, Valazquez JG, Burdick KH: Precise evaluation of topically applied corticosteroid potency . Arch Dermatol 101:531-537, 1970.Crossref 9. Poulsen BJ: The use of models in estimating vehicle effects on the activity of topical corticosteroid formulations . Br J Dermatol 82 ( (suppl 6) ):49-52, 1970.Crossref 10. Christie GA, Moore-Robinson M: Vehicle assessment-methodology and results . Br J Dermatol 82 ( (suppl 6) ):93-98, 1970.Crossref 11. Busse MJ, et al: Release of betamethasone derivatives from ointments: In vivo and in vitro studies . Br J Dermatol 81( (suppl 4) ):103-112, 1969.Crossref 12. Ostrenga J, Steinmetz C, Poulsen B: Significance of vehicle composition: I. Relationship between topical vehicle composition, skin penetrability, and clinical efficacy . J Pharm Sci 60:1175-1179, 1971.Crossref 13. Ostrenga J, et al: Vehicle design for a new topical steroid, fluocinonide . J Invest Dermatol 56:392-399, 1971.Crossref 14. Katz M, Poulsen BJ: Corticoid, vehicle, and skin interaction in percutaneous absorption . J Soc Cosmet Chem 23:565-590, 1972. 15. Pepler AF, Woodford R, Morrison JC: The influence of vehicle composition on the vasoconstrictor activity of betamethasone 17-benzoate . Br J Dermatol 85:171-176, 1971.Crossref 16. Coldman MF, Lockerbie L, Laws EA: The evaluation of several topical corticosteroid formulations in the blanching test . Br J Dermatol 85:381-387, 1971.Crossref 17. Coldman MF, Lockerbie L, Laws EA: The evaluation of a novel corticosteroid formulation, fluocinonide in "FAPG" base, in the blanching test . Br J Dermatol 85:573-576, 1971.Crossref 18. Stewart WD, et al: Problems in selection of topical anti-inflammatory corticosteroids . Can Med Assoc J 108:33-38, 1973. 19. Burdick KH, Poulsen B, Place VA: Extemporaneous formulation of corticosteroids for topical usage . JAMA 211:462-466, 1970.Crossref 20. Siegal S: Nonparametric Statistics for the Behavioral Sciences . New York, McGraw-Hill Book Co Inc, 1956, p 63. 21. Daniels F Jr, van der Leun JC: Problems in quantifying skin reactions . Arch Dermatol 97:553-565, 1968.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Paired Comparison Vasoconstrictor Assays: A Comparison of Methods for the Determination of Relative Vasoconstrictor Potency of Topically Applied Corticosteroids

Loading next page...
 
/lp/american-medical-association/paired-comparison-vasoconstrictor-assays-a-comparison-of-methods-for-ds5agLZmGZ
Publisher
American Medical Association
Copyright
Copyright © 1974 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1974.01630030027005
Publisher site
See Article on Publisher Site

Abstract

Abstract The human vasoconstrictor assay, which is frequently used for potency comparisons of topically applied corticosteroids, relies on the visual measurement of skin blanching produced at the application site. In this report, three different methods of visual measurement were compared. A technique involving paired comparisons of vasoconstriction at adjacent sites was found to improve the assessment of relative vasoconstrictor activity. Corticosteroid creams containing 0.05% fluocinonide, 0.1% betamethasone valerate, and 1% hydrocortisone were evaluated in this study. References 1. McKenzie AW, Stoughton RB: Method for comparing percutaneous absorption of steroids . Arch Dermatol 86:608-610, 1962.Crossref 2. McKenzie AW: Percutaneous absorption of steroids . Arch Dermatol 86:611-614, 1962.Crossref 3. McKenzie AW, Atkinson RM: Topical activities of betamethasone esters in man . Arch Dermatol 89:741-746, 1964.Crossref 4. Stoughton RB: Bioassay system for formulations of topically applied glucocorticosteroids . Arch Dermatol 106:825-827, 1972.Crossref 5. Burdick K, et al: Corticosteroid ointments: Comparison by two human bioassays . Curr Ther Res 15:233-242, 1973. 6. Child KJ, et al: Vasoconstrictor and systemic activities of topical steroids . Arch Dermatol 97:407-410, 1968.Crossref 7. Stoughton RB: Vasoconstrictor activity and percutaneous absorption of glucocorticosteroids . Arch Dermatol 99:753-756, 1969.Crossref 8. Place VA, Valazquez JG, Burdick KH: Precise evaluation of topically applied corticosteroid potency . Arch Dermatol 101:531-537, 1970.Crossref 9. Poulsen BJ: The use of models in estimating vehicle effects on the activity of topical corticosteroid formulations . Br J Dermatol 82 ( (suppl 6) ):49-52, 1970.Crossref 10. Christie GA, Moore-Robinson M: Vehicle assessment-methodology and results . Br J Dermatol 82 ( (suppl 6) ):93-98, 1970.Crossref 11. Busse MJ, et al: Release of betamethasone derivatives from ointments: In vivo and in vitro studies . Br J Dermatol 81( (suppl 4) ):103-112, 1969.Crossref 12. Ostrenga J, Steinmetz C, Poulsen B: Significance of vehicle composition: I. Relationship between topical vehicle composition, skin penetrability, and clinical efficacy . J Pharm Sci 60:1175-1179, 1971.Crossref 13. Ostrenga J, et al: Vehicle design for a new topical steroid, fluocinonide . J Invest Dermatol 56:392-399, 1971.Crossref 14. Katz M, Poulsen BJ: Corticoid, vehicle, and skin interaction in percutaneous absorption . J Soc Cosmet Chem 23:565-590, 1972. 15. Pepler AF, Woodford R, Morrison JC: The influence of vehicle composition on the vasoconstrictor activity of betamethasone 17-benzoate . Br J Dermatol 85:171-176, 1971.Crossref 16. Coldman MF, Lockerbie L, Laws EA: The evaluation of several topical corticosteroid formulations in the blanching test . Br J Dermatol 85:381-387, 1971.Crossref 17. Coldman MF, Lockerbie L, Laws EA: The evaluation of a novel corticosteroid formulation, fluocinonide in "FAPG" base, in the blanching test . Br J Dermatol 85:573-576, 1971.Crossref 18. Stewart WD, et al: Problems in selection of topical anti-inflammatory corticosteroids . Can Med Assoc J 108:33-38, 1973. 19. Burdick KH, Poulsen B, Place VA: Extemporaneous formulation of corticosteroids for topical usage . JAMA 211:462-466, 1970.Crossref 20. Siegal S: Nonparametric Statistics for the Behavioral Sciences . New York, McGraw-Hill Book Co Inc, 1956, p 63. 21. Daniels F Jr, van der Leun JC: Problems in quantifying skin reactions . Arch Dermatol 97:553-565, 1968.Crossref

Journal

Archives of DermatologyAmerican Medical Association

Published: Mar 1, 1974

References