Abstract • A total of 26 albino guinea pigs were treated with 200 mg/kg/day of tobramycin sulfate. Animals were killed at various intervals of up to three weeks after seven days treatment. Outer hair cell (OHC) loss of the organ of Corti was evaluated by surface preparation techniques, and glycogen was assessed with PATCO- and PAS-stained sections. We have concluded that permanent damage of OHCs is most prevalent in areas that normally have the least amount and the smallest granule size of glycogen. With treatment, these susceptible areas are the least responsive in terms of an early increase in glycogen production. Furthermore, by 21 days after seven days of treatment, these same areas will reveal an almost total loss of glycogen at a time when damage is maximal. (Arch Otolaryngol 102:154-159, 1976) References 1. Thalmann R, Miyoshi MD, Thalmann I: The influence of ischemia upon the energy reserves of inner ear tissues . Laryngoscope 82:2249-2272, 1972.Crossref 2. Thalmann R, Miyoshi T, Rauchbach E: Biochemical correlates of inner ear ischemia , in Lorenzo AJD (ed): Vascular Disorders and Hearing Defects . Baltimore, University Park Press, 1973, p 219. 3. Falbe-Hansen J: On glycogen in the cochlear duct of foetuses and of young albino rats . Acta Otolaryngol 63:340-346, 1967.Crossref 4. Falbe-Hansen J: Histochemical studies on glycogen in the cochlea of drug-injected guinea pigs and alloxan diabetic white rats . Acta Otolaryngol 188( (suppl) ):108-118, 1964.Crossref 5. Ishii D, Takahashi T, Balogh K: Glycogen in the inner ear after acoustic stimulation . Acta Otolaryngol 67:573-582, 1969.Crossref 6. Vinnikov YA, Titova L: The Organ of Corti, its Histopathology and Histochemistry . New York, Consultants Bureau, 1964, pp 111-123. 7. Prazma J, Postma D, Pecorak J, et al: Ototoxicity of tobramycin sulfate . Laryngoscope , to be published. 8. Hanker JS, Seaman LP, Weiss HP, et al: Osmiophilic reagent: New cytochemical principle for light and electron microscopy . Science 146:1039-1043, 1964.Crossref 9. Snodgress AB, Dorsey CH, Bailey W, et al: Conventional histopathologic staining techniques compatible with epon-embedded osmicated tissue . Lab Invest 26:329-337, 1972. 10. Falbe-Hansen J, Thomsen E: Histochemical studies on glycogen in the cochlea of the normal guinea pig . Acta Otolarynogol 56:429-436, 1963.Crossref 11. Matschinsky FM, Thalmann R: Quantitative histochemistry of microscopic structures of the cochlea: II. Ischemic alterations of levels of glycolytic intermediates and cofactors in the organ of Corti and stria vascularis . Ann Otol Rhinol Laryngol 76:638-646, 1967. 12. Matschinsky F: Energy metabolism of the microscopic structures of the cochlea, the retina, and the cerebellum . Adv Biochem Psychopharmacol 2:217-243, 1970. 13. Kugler JH, Wilkenson WTC: A relation between total glycogen content of ox myocardium and its histochemical demonstration . J Histochem Cytochem 7:398-402, 1959.Crossref 14. Nakamura F: Electrophysiological and cytochemical study on ototoxicity of dihydrostreptomycin . Am Otol Rhinol Laryngol 66:1080-1112, 1957. 15. Gonzalez G, Miller N, Wosilewski V: Progressive neuro-ototoxicity of kanamycin . Ann Otol Rhinol Laryngol 81:127-131, 1972. 16. Koide Y, Yoshida M, Konno M, et al: Some aspects of the biochemistry of acoustic trauma . Ann Otol Rhinol Laryngol 69:661-697, 1960. 17. Beck C: Protein and ribonucleic acid metabolism in the cochlea . Arch Otolaryngol 81:548-552, 1965.Crossref 18. Robbins SL: Pathologic Basis of Disease . Philadelphia, WB Saunders Co, 1974. 19. Stange G, Holz E, Terayama Y, et al: Korrelation morphologischer, biochemischer and elektro-physiologischer: Untersuchungsergebnisse des akustischen systems . Arch Klin Exp Ohren Nasen Kehlkopfheilkd 186:229-246, 1966.Crossref 20. Cameron R, Spector WG: The Chemistry of the Injured Cell . Springfield, Ill, Charles C Thomas, Publishers, 1961, p 35.
Archives of Otolaryngology – American Medical Association
Published: Mar 1, 1976