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Origin of Plasma Protoporphyrin in Patients With Erythropoietic Protoporphyria

Origin of Plasma Protoporphyrin in Patients With Erythropoietic Protoporphyria Abstract To the Editor.— In their reply to our communication in the August 1980 Archives (116:863-864), Poh-Fitzpatrick and Mathews-Roth still insist that the studies of Piomelli et al1 were partly in vivo. To resolve this semantic discordance, we will present our concept of an in vivo biochemical experiment. We visualize (at least) the following two broad types of study: (1) Collect specimens from a live animal, make the animal undergo stress, and collect specimens at recorded intervals. The specimens are then analyzed for a metabolite of interest to determine the effect of the experimental stress on turnover of the metabolite. (2) Administer radiotracers to a live animal, collect specimens, and monitor the rise and fall of radioactivity in a metabolite of interest. This type of experiment was conducted by Scholnick et al2 and by Schwartz et al3 to monitor protoporphyrin (PP) turnover in patients with erythropoietic protoporphyria (EPP). References 1. Piomelli S, Lamola AA, Poh-Fitzpatrick MB, et al: Erythropoietic protoporphyria and lead intoxication: The molecular basis for difference in cutaneous photosensitivity: I. Different rates of disappearance of protoporphyrin from the erythrocytes, both in vivo and in vitro . J Clin Invest 1975;56:1519-1527.Crossref 2. Scholnick P, Marver HS, Schmid R: Erythropoietic protoporphyria: Evidence for multiple sites of excess protoporphyrin formation . J Clin Invest 1971;50:203-207.Crossref 3. Schwartz S, Johnson JA, Stephenson BD, et al: Erythropoietic defects in protoporphyria: A study of factors involved in labelling of porphyrins and bile pigments fron ALA-3H and glycine-14C . J Lab Clin Med 1971;78:411-434. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Origin of Plasma Protoporphyrin in Patients With Erythropoietic Protoporphyria

Archives of Dermatology , Volume 117 (9) – Sep 1, 1981

Origin of Plasma Protoporphyrin in Patients With Erythropoietic Protoporphyria

Abstract

Abstract To the Editor.— In their reply to our communication in the August 1980 Archives (116:863-864), Poh-Fitzpatrick and Mathews-Roth still insist that the studies of Piomelli et al1 were partly in vivo. To resolve this semantic discordance, we will present our concept of an in vivo biochemical experiment. We visualize (at least) the following two broad types of study: (1) Collect specimens from a live animal, make the animal undergo stress, and collect specimens at recorded...
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References (3)

Publisher
American Medical Association
Copyright
Copyright © 1981 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1981.01650090006007
Publisher site
See Article on Publisher Site

Abstract

Abstract To the Editor.— In their reply to our communication in the August 1980 Archives (116:863-864), Poh-Fitzpatrick and Mathews-Roth still insist that the studies of Piomelli et al1 were partly in vivo. To resolve this semantic discordance, we will present our concept of an in vivo biochemical experiment. We visualize (at least) the following two broad types of study: (1) Collect specimens from a live animal, make the animal undergo stress, and collect specimens at recorded intervals. The specimens are then analyzed for a metabolite of interest to determine the effect of the experimental stress on turnover of the metabolite. (2) Administer radiotracers to a live animal, collect specimens, and monitor the rise and fall of radioactivity in a metabolite of interest. This type of experiment was conducted by Scholnick et al2 and by Schwartz et al3 to monitor protoporphyrin (PP) turnover in patients with erythropoietic protoporphyria (EPP). References 1. Piomelli S, Lamola AA, Poh-Fitzpatrick MB, et al: Erythropoietic protoporphyria and lead intoxication: The molecular basis for difference in cutaneous photosensitivity: I. Different rates of disappearance of protoporphyrin from the erythrocytes, both in vivo and in vitro . J Clin Invest 1975;56:1519-1527.Crossref 2. Scholnick P, Marver HS, Schmid R: Erythropoietic protoporphyria: Evidence for multiple sites of excess protoporphyrin formation . J Clin Invest 1971;50:203-207.Crossref 3. Schwartz S, Johnson JA, Stephenson BD, et al: Erythropoietic defects in protoporphyria: A study of factors involved in labelling of porphyrins and bile pigments fron ALA-3H and glycine-14C . J Lab Clin Med 1971;78:411-434.

Journal

Archives of DermatologyAmerican Medical Association

Published: Sep 1, 1981

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