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Ever since Gaffky, in 1894, succeeded in cultivating upon artificial media, the bacillus discovered in typhoidal tissues by Eberth, many efforts have been made by bacteriologists and clinicians to isolate the organism from the fluids and excretions of the living body, notably the blood, urine and feces. Up to 1891 the investigations of a Pfeiffer, Simmonds, and especially Karlinski, had shown the task to be apparently a very simple one. Unfortunately, however, the value of the results of these observers is open to very serious doubts; for the methods employed in proving the cultures thus obtained, were, according to our present standards, entirely insufficient, and it is more than probable that a great many of the bacilli thus isolated were not typhoid bacilli at all, but others of the same group, most probably varieties of the colon bacillus. In investigating stools the greatest difficulties have arisen from the other bacteria
JAMA – American Medical Association
Published: Jul 3, 1897
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