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Mucopolysaccharide Staining in Experimental Corneal Edema

Mucopolysaccharide Staining in Experimental Corneal Edema Abstract Ashton1 has shown that the staining characteristics of the rabbit corneal mucopolysaccharides are altered by the swelling which follows suspension of the excised cornea in distilled water. A well-standardized corneal lesion resulting in corneal edema can be produced in the live rabbit by instilling into the anterior chamber agents which are toxic to the endothelium. Such an experimental preparation has greater similarities to clinically encountered corneal edema and offers obvious advantages in investigative maneuverability over an in vitro prepared specimen. This investigation demonstrates some of the mucopolysaccharide staining properties of such in vivo edematous corneas produced by the anterior chamber injection of one endothelial toxic substance, Janus green B. Additionally, some observations are recorded on the staining reaction of the normal and the edematous cornea after the subconjunctival administration of methylprednisolone acetate (Depo-Medrol). Materials and Methods Albino rabbits weighing approximately 2 kg (4.4 lb) were used. Preliminary experiments to References 1. Ashton, N.: Histochemical Studies on the Normal and Swollen Cornea , Amer J Ophthal 47:229, 1959. 2. Nicholas, J.P.: Whole Mounts of the Corneal Endothelium , Arch Ophthal 73:555, 1965.Crossref 3. Flocks, M.: The Anatomy of the Trabecular Meshwork as Seen in Tangential Section , Arch Ophthal 56:708, 1956.Crossref 4. Lillie, R.D.: Histopathologic Technic and Practical Histochemistry , ed 2, New York: Blakiston Co., Inc., 1954. 5. Lison, L.: Alcian Blue 8G with Chlorantine Fast Red 5B: A Technic for Selective Staining of Mucopolysaccharides , Stain Techn 29:131, 1954. 6. Rinehart, J.F., and Abul-Haj, S.K.: An Improved Method for Histologic Demonstration of Acid Mucopolysaccharides in Tissues , Arch Path 52:189, 1951. 7. Hitchkiss, R.D.: A Microchemical Reaction Resulting in the Staining of Polysaccharide Structures in Fixed Tissue Preparations , Arch Biochem 16:131, 1948. 8. Mowry, R.W.: The Special Value of Methods That Color Both Acidic and Vicinal Hydroxyl Groups in the Histochemical Study of Mucins, With Revised Directions for the Colloidal Iron Stain, the Use of Alcian Blue 8GS and Their Combinations With the Periodic Acid-Schiff Reaction , Ann NY Acad Sci 106:402, 1963.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Mucopolysaccharide Staining in Experimental Corneal Edema

Archives of Ophthalmology , Volume 76 (2) – Aug 1, 1966

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Publisher
American Medical Association
Copyright
Copyright © 1966 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1966.03850010240016
Publisher site
See Article on Publisher Site

Abstract

Abstract Ashton1 has shown that the staining characteristics of the rabbit corneal mucopolysaccharides are altered by the swelling which follows suspension of the excised cornea in distilled water. A well-standardized corneal lesion resulting in corneal edema can be produced in the live rabbit by instilling into the anterior chamber agents which are toxic to the endothelium. Such an experimental preparation has greater similarities to clinically encountered corneal edema and offers obvious advantages in investigative maneuverability over an in vitro prepared specimen. This investigation demonstrates some of the mucopolysaccharide staining properties of such in vivo edematous corneas produced by the anterior chamber injection of one endothelial toxic substance, Janus green B. Additionally, some observations are recorded on the staining reaction of the normal and the edematous cornea after the subconjunctival administration of methylprednisolone acetate (Depo-Medrol). Materials and Methods Albino rabbits weighing approximately 2 kg (4.4 lb) were used. Preliminary experiments to References 1. Ashton, N.: Histochemical Studies on the Normal and Swollen Cornea , Amer J Ophthal 47:229, 1959. 2. Nicholas, J.P.: Whole Mounts of the Corneal Endothelium , Arch Ophthal 73:555, 1965.Crossref 3. Flocks, M.: The Anatomy of the Trabecular Meshwork as Seen in Tangential Section , Arch Ophthal 56:708, 1956.Crossref 4. Lillie, R.D.: Histopathologic Technic and Practical Histochemistry , ed 2, New York: Blakiston Co., Inc., 1954. 5. Lison, L.: Alcian Blue 8G with Chlorantine Fast Red 5B: A Technic for Selective Staining of Mucopolysaccharides , Stain Techn 29:131, 1954. 6. Rinehart, J.F., and Abul-Haj, S.K.: An Improved Method for Histologic Demonstration of Acid Mucopolysaccharides in Tissues , Arch Path 52:189, 1951. 7. Hitchkiss, R.D.: A Microchemical Reaction Resulting in the Staining of Polysaccharide Structures in Fixed Tissue Preparations , Arch Biochem 16:131, 1948. 8. Mowry, R.W.: The Special Value of Methods That Color Both Acidic and Vicinal Hydroxyl Groups in the Histochemical Study of Mucins, With Revised Directions for the Colloidal Iron Stain, the Use of Alcian Blue 8GS and Their Combinations With the Periodic Acid-Schiff Reaction , Ann NY Acad Sci 106:402, 1963.Crossref

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Aug 1, 1966

References