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Microbial Contamination of Donor Eyes: A Retrospective Study

Microbial Contamination of Donor Eyes: A Retrospective Study Abstract • The culture results of 4,167 donor eyes received over a two-year period are evaluated. Irrigation of the cornea and conjunctiva with 20 mL of sterile saline prior to enucleation decreased the incidence of bacterial contamination to 12.4%. The major contaminant is Staphylococcus epidermidis (66.4%). The incidence of endophthalmitis in the 1,880 corneal transplants performed during this time was 0.1%. The significance of irrigation and antibiotic use in processing donor corneas is discussed. References 1. Shaw EL, Aquavella JV: Pneumococcal endophthalmitis following grafting of corneal tissue from a (cadaver) kidney donor . Ann Ophthalmol 1977;9:435-440. 2. Le Francois M, Baum JL: Flavobacterium endophthalmitis following keratoplasty . Arch Ophthalmol 1976;94:1907-1909.Crossref 3. Larsen PA, Lindstrom RL, Doughman DJ: Torulopsis glabrata endophthalmitis after keratoplasty with an organ-cultured cornea . Arch Ophthalmol 1978;96:1019-1022.Crossref 4. Khodadoust AA, Franklin RM: Transfer of bacterial infection by donor cornea in penetrating keratoplasty . Am J Ophthalmol 1979;87:130-132. 5. Escapini H, Olson RJ, Kaufman HE: Donor cornea contamination with McCarey-Kaufman medium preservation . Am J Ophthalmol 1979; 88:59-62. 6. Polack FM, Locatcher-Khorazo D, Gutlerrez E: Bacteriologic study of 'donor' eyes . Arch Ophthalmol 1967;78:219-225.Crossref 7. Boberg-Ans J, Badsberg E, Rasmussen J: Frequency of infection in donor eyes post mortem: A method of obtaining sterile eyes for corneal grafting . Br J Ophthalmol 1962;46:365-368.Crossref 8. Schimmelpfennig B, Hurzeler R: Bacterial flora of stored human donor corneas after antibiotic treatment . Albrecht Von Graefes Arch Klin Exp Ophthalmol 1977;202:181-185.Crossref 9. Goldman KN, Centifanto Y, Kaufman HE, et al: Prevention of surface bacterial contamination of donor corneas . Arch Ophthalmol 1980; 96:2277-2280.Crossref 10. Rollins HJ, Stocker FW: Bacterial flora and preoperative treatment of donor corneas . Am J Ophthalmol 1965;59:247-249. 11. Locatcher-Khorazo D, Seegal BC: Microbiology of the Eye . St Louis, CV Mosby Co, 1972, pp 13-23. 12. Tomar VPS, Sharma OP, Joshi K: Bacterial and fungal flora of normal conjunctiva . Ann Ophthalmol 1971;3:669-671. 13. Allen HF, Mangiaracine AB: Bacterial endophthalmitis after cataract extraction . Arch Ophthalmol 1964;72:454-462.Crossref 14. Christy NE, Lall P: Postoperative endophthalmitis following cataract surgery . Arch Ophthalmol 1973;90:361-379.Crossref 15. Forster RK, Abbott RL, Gelender H: Management of infectious endophthalmitis . Ophthalmology 1980;87:313-319.Crossref 16. Mascarella K, Cavanagh HD: Penetrating keratoplasty using McCarey-Kaufman preserved corneal tissue . South Med J 1979;72:1268-1271.Crossref 17. Baum J, Barza M, Kane A: Efficacy of penicillin G, cefazolin, and gentamicin in M-K medium at 4 °C . Arch Ophthalmol 1978;96:1262-1264.Crossref 18. Doctor D, Hughes I: Prophylactic use of Neosporin for donor eyes . Am J Ophthalmol 1958;46:351-353. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Microbial Contamination of Donor Eyes: A Retrospective Study

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Publisher
American Medical Association
Copyright
Copyright © 1982 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1982.01030040589006
Publisher site
See Article on Publisher Site

Abstract

Abstract • The culture results of 4,167 donor eyes received over a two-year period are evaluated. Irrigation of the cornea and conjunctiva with 20 mL of sterile saline prior to enucleation decreased the incidence of bacterial contamination to 12.4%. The major contaminant is Staphylococcus epidermidis (66.4%). The incidence of endophthalmitis in the 1,880 corneal transplants performed during this time was 0.1%. The significance of irrigation and antibiotic use in processing donor corneas is discussed. References 1. Shaw EL, Aquavella JV: Pneumococcal endophthalmitis following grafting of corneal tissue from a (cadaver) kidney donor . Ann Ophthalmol 1977;9:435-440. 2. Le Francois M, Baum JL: Flavobacterium endophthalmitis following keratoplasty . Arch Ophthalmol 1976;94:1907-1909.Crossref 3. Larsen PA, Lindstrom RL, Doughman DJ: Torulopsis glabrata endophthalmitis after keratoplasty with an organ-cultured cornea . Arch Ophthalmol 1978;96:1019-1022.Crossref 4. Khodadoust AA, Franklin RM: Transfer of bacterial infection by donor cornea in penetrating keratoplasty . Am J Ophthalmol 1979;87:130-132. 5. Escapini H, Olson RJ, Kaufman HE: Donor cornea contamination with McCarey-Kaufman medium preservation . Am J Ophthalmol 1979; 88:59-62. 6. Polack FM, Locatcher-Khorazo D, Gutlerrez E: Bacteriologic study of 'donor' eyes . Arch Ophthalmol 1967;78:219-225.Crossref 7. Boberg-Ans J, Badsberg E, Rasmussen J: Frequency of infection in donor eyes post mortem: A method of obtaining sterile eyes for corneal grafting . Br J Ophthalmol 1962;46:365-368.Crossref 8. Schimmelpfennig B, Hurzeler R: Bacterial flora of stored human donor corneas after antibiotic treatment . Albrecht Von Graefes Arch Klin Exp Ophthalmol 1977;202:181-185.Crossref 9. Goldman KN, Centifanto Y, Kaufman HE, et al: Prevention of surface bacterial contamination of donor corneas . Arch Ophthalmol 1980; 96:2277-2280.Crossref 10. Rollins HJ, Stocker FW: Bacterial flora and preoperative treatment of donor corneas . Am J Ophthalmol 1965;59:247-249. 11. Locatcher-Khorazo D, Seegal BC: Microbiology of the Eye . St Louis, CV Mosby Co, 1972, pp 13-23. 12. Tomar VPS, Sharma OP, Joshi K: Bacterial and fungal flora of normal conjunctiva . Ann Ophthalmol 1971;3:669-671. 13. Allen HF, Mangiaracine AB: Bacterial endophthalmitis after cataract extraction . Arch Ophthalmol 1964;72:454-462.Crossref 14. Christy NE, Lall P: Postoperative endophthalmitis following cataract surgery . Arch Ophthalmol 1973;90:361-379.Crossref 15. Forster RK, Abbott RL, Gelender H: Management of infectious endophthalmitis . Ophthalmology 1980;87:313-319.Crossref 16. Mascarella K, Cavanagh HD: Penetrating keratoplasty using McCarey-Kaufman preserved corneal tissue . South Med J 1979;72:1268-1271.Crossref 17. Baum J, Barza M, Kane A: Efficacy of penicillin G, cefazolin, and gentamicin in M-K medium at 4 °C . Arch Ophthalmol 1978;96:1262-1264.Crossref 18. Doctor D, Hughes I: Prophylactic use of Neosporin for donor eyes . Am J Ophthalmol 1958;46:351-353.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Oct 1, 1982

References