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Abstract • We have previously reported an in vitro model demonstrating decreased hepatocyte protein synthesis following co-culture with macrophage-rich peritoneal cells (MRPC) in Sprague-Dawley rats. These observations have been proposed by us and others to represent a possible model for macrophage- or Kupffer cell—mediated modulation of hepatocyte function. Such a mechanism may play a role in the etiology of hepatic failure in sepsis. This effect is shown in this investigation to be completely blocked by dexamethasone at concentrations equal to or greater than 10 −9M. The presence of lymphocyte-activating factor in the MRPC-conditioned medium is suppressed at the same concentration of dexamethasone. Resident MRPC were shown to secrete significant amounts of lymphocyte-activating factor activity without further in vitro activation. 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Archives of Surgery – American Medical Association
Published: Oct 1, 1986
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