Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Lysis of Squamous Cell Carcinoma Via Antibody-Dependent Cellular Cytotoxicity

Lysis of Squamous Cell Carcinoma Via Antibody-Dependent Cellular Cytotoxicity Abstract • We have developed a system in which antibody-dependent cellular cytotoxicity (ADCC) can be consistently demonstrated against squamous cell carcinoma (SCC) of the head and neck. Serum samples from patients with pemphigus vulgaris (PV) provided antibodies for ADCC. The effector cells were lymphocytes from the peripheral blood of healthy human donors. The SCC cell lines served as targets. Lysis of SCC, as measured by 51Cr release, was significantly enhanced by the presence of serum from patients with PV, but not by healthy human serum. Serum alone produced no target cell killing. Nonepithelial cell lines were not affected by the presence of PV antibodies. The results demonstrate that SCC is susceptible to ADCC. Thus, tumor-specific antibodies may have a role in the treatment of cancer of the head and neck. This system can serve as a positive control for further testing of ADCC against SCC. (Arch Otolaryngol Head Neck Surg 1989;115:669-676) References 1. Norris DA, Lee LA: Antibody-dependent cellular cytotoxicity and skin disease . J Invest Dermatol 1985;85( (suppl) ):165s-175s.Crossref 2. Krause CJ, Carey TE, Ott RW, et al: Human squamous cell carcinoma: Establishment and characterization of new permanent cell lines . Arch Otolaryngol Head Neck Surg 1981;107:703-710.Crossref 3. Fast LD, Hansen JA, Newman W: Evidence for T cell nature and heterogeneity within natural killer (NK) and antibody-dependent cellular cytotoxicity (ADCC) effectors: A comparison with cytolytic T lymphocytes (CTL) . J Immunol 1981;127:448-452. 4. Kay HD, Bonnard GD, West WH, et al: A functional comparison of human Fc-receptor bearing lymphocytes active in natural cytotoxicity and antibody-dependent cellular cytotoxicity . J Immunol 1977;118:2058-2066. 5. Zielske JV, Golub SH: Fetal calf seruminduced blastogenesis and cytotoxic responses of human lymphocytes . Cancer Res 1976;36:3842-3846. 6. Schiltz JR, Michel B: Production of epidermal acantholysis in normal human skin in vitro by the IgG fraction from pemphigus serum . J Invest Dermatol 1976;67:254-260.Crossref 7. Stanley JR, Koulu L, Thivolet C: Distinction between epidermal antigens binding pemphigus vulgaris and pemphigus foliaceous autoantibodies . J Clin Invest 1984;74:313-320.Crossref 8. Stanley JR, Koulu L, Klaus-Kovton V, et al: A monoclonal antibody to the desmosomal glycoprotein desmoglein I binds the same polypeptide as human autoantibodies in pemphigus foliaceous . J Immunol 1986;136:1227-1230. 9. Kipps TJ, Parham P, Punt J, et al: Importance of immunoglobulin isotype in human antibody-dependent cell-mediated cytotoxicity directed by murine monoclonal antibodies . J Exp Med 1985;161:1-17.Crossref 10. Christiaansen JE, Burnside SS, Sears DW: Apparent sensitivity of human K lymphocytes to the spatial orientation and organization of target cell-bound antibodies as measured by the efficiency of antibody-dependent cellular cytotoxicity (ADCC) . J Immunol 1987;138:2236-2243. 11. Shiloni E, Eisenthal A, Sachs D, et al: Antibody-dependent cellular cytotoxicity mediated by murine lymphocytes activated in recombinant interleukin 2 . J Immunol 1987;138:1992-1998. 12. Ortaldo JR, Woodhouse C, Morgan AC, et al: Analysis of effector cells in human antibody-dependent cellular cytotoxicity with murine monoclonal antibodies . J Immunol 1987;138:3566-3572. 13. Slezak S, Symer DE, Shin HS: Platelet-mediated cytotoxicity: Role of antibody and C3, and localization of the cytotoxic system in membranes . J Exp Med 1987;166:489-505.Crossref 14. Herlyn D, Koprowski H: IgG2a monoclonal antibodies inhibit human tumor growth through interaction with effector cells . Proc Natl Acad Sci USA 1982;79:4761-4765.Crossref 15. Berlinger NT, Hilal EY, Oettgen HF, et al: Deficient cell-mediated immunity in head and neck cancer patients secondary to autologous immune cells . Laryngoscope 1978;88:470-481.Crossref 16. Alexander E, Henkart P: The adherence of human Fc receptor–bearing lymphocytes to antigen-antibody complexes: II. Morphologic alterations induced by the substrate . J Exp Med 1976;143:329-347.Crossref 17. Simeone CB, Henkart P: Permeability changes induced in erythrocyte ghost targets by antibody-dependent cytotoxic effector cells: Evidence for membrane pores . J Immunol 1980; 124:954-963. 18. Malavasi F, Tetta C, Funaro A, et al: Fc receptor triggering induces expression of surface antigens and the release of platelet-activating factor in large granular lymphocytes . Proc Natl Acad Sci USA 1986;83:2443-2447.Crossref 19. Kondo LL, Rosenau W, Wora DW: Role of lymphotoxin in antibody-dependent cell-mediated cytotoxicity (ADCC) . J Immunol 1981; 126:1131-1133. 20. Duke RC, Chervenak R, Cohen JJ: Endogenous endonuclease-induced DNA fragmentation: An early event in cell-mediated lysis . Proc Natl Acad Sci USA 1983;80:6361-6365.Crossref 21. Masui H, Moroyama T, Mendelsohn J: Mechanism of antitumor activity in mice for anti-epidermal growth factor receptor monoclonal antibodies with different isotypes . Cancer Res 1986;46:5992-5998. 22. Herlyn D, Herlyn M, Steplewski Z, et al: Monoclonal anti-human tumor antibodies of six isotypes in cytotoxic reactions with human and murine effector cells . Cell Immunol 1985;92:105-114.Crossref 23. Weiner LM, Steplewski Z, Koprowski H, et al: Biological effects of gamma interferon pretreatment followed by monoclonal antibody 17-1A administration in patients with gastrointestinal carcinoma . Hybridoma 1986;5( (suppl) ):s65-s77. 24. Houghton AN, Mintzer D, Cordon-Cardo C, et al: Mouse monoclonal IgG3 antibody detecting GD3 ganglioside: A phase I trail in patients with malignant melanoma . Proc Natl Acad Sci USA 1985;82:1242-1246.Crossref 25. Sears HF, Mattis J, Herlyn D, et al: Phase-I clinical trial of monoclonal antibody in treatment of gastrointestinal tumors . Lancet 1982; 1:762-765.Crossref 26. Carey TE, Kimmel KA, Schwartz DR, et al: Antibodies to human squamous cell carcinoma . Otolaryngol Head Neck Surg 1983;91:482-491. 27. Koneval T, Applebaum E, Popovic D, et al: Demonstration of immunoglobulin in tumor and marginal tissues of squamous cell carcinomas of the head and neck . JNCI 1977;59:1089-1093. 28. Wolf GT, Hudson JL, Peterson KA, et al: Lymphocyte subpopulations infiltrating squamous carcinoma of the head and neck: Correlations with extent of tumor and prognosis . Otolaryngol Head Neck Surg 1986;95:142-152. 29. Danielson JR, Franklin WA: Characterization of antibody-forming cells adjacent to laryngeal carcinoma . Arch Otolaryngol Head Neck Surg 1984;110:327-328.Crossref 30. Wanebo HJ: Immunobiology of head and neck cancer: Basic concepts . Head Neck Surg 1979;2:42-55.Crossref 31. Mathew GD, Qualtiere LF, Neel HB, et al: Immunoglobulin A antibody to Epstein-Barr viral antigens and prognosis in nasopharyngeal carcinoma . Otolaryngol Head Neck Surg 1980;88:52-57. 32. Troye M, Hanssen Y, Paulie S, et al: Lymphocyte-mediated lysis of tumor cells in vitro (ADCC) induced by serum antibodies from patients with urinary bladder carcinoma or from controls . Int J Cancer 1980;25:45-51.Crossref 33. Heo DS, Whiteside TL, Johnson JT, et al: Long-term interleukin 2-dependent growth and cytotoxicity activity of tumor infiltrating lymphocytes from human squamous cell carcinomas of the head and neck . Cancer Res 1987;47:6353-6362. 34. Ariyan S, Krizek TJ, Mitchell MS: Identification of squamous cell carcinoma of the head and neck by tissue culture and immunological testing . Plast Reconstr Surg 1977;59:386-394.Crossref 35. Aust JC, Rabuzzi D, Reed G: Tissuecultured head and neck tumors: Their use in vitro assays of immune response . Trans Am Acad Ophthalmol Otolaryngol 1977;84:603-608. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Otolaryngology - Head & Neck Surgery American Medical Association

Lysis of Squamous Cell Carcinoma Via Antibody-Dependent Cellular Cytotoxicity

Loading next page...
 
/lp/american-medical-association/lysis-of-squamous-cell-carcinoma-via-antibody-dependent-cellular-aAXXXx2pAS
Publisher
American Medical Association
Copyright
Copyright © 1989 American Medical Association. All Rights Reserved.
ISSN
0886-4470
eISSN
1538-361X
DOI
10.1001/archotol.1989.01860300023010
Publisher site
See Article on Publisher Site

Abstract

Abstract • We have developed a system in which antibody-dependent cellular cytotoxicity (ADCC) can be consistently demonstrated against squamous cell carcinoma (SCC) of the head and neck. Serum samples from patients with pemphigus vulgaris (PV) provided antibodies for ADCC. The effector cells were lymphocytes from the peripheral blood of healthy human donors. The SCC cell lines served as targets. Lysis of SCC, as measured by 51Cr release, was significantly enhanced by the presence of serum from patients with PV, but not by healthy human serum. Serum alone produced no target cell killing. Nonepithelial cell lines were not affected by the presence of PV antibodies. The results demonstrate that SCC is susceptible to ADCC. Thus, tumor-specific antibodies may have a role in the treatment of cancer of the head and neck. This system can serve as a positive control for further testing of ADCC against SCC. (Arch Otolaryngol Head Neck Surg 1989;115:669-676) References 1. Norris DA, Lee LA: Antibody-dependent cellular cytotoxicity and skin disease . J Invest Dermatol 1985;85( (suppl) ):165s-175s.Crossref 2. Krause CJ, Carey TE, Ott RW, et al: Human squamous cell carcinoma: Establishment and characterization of new permanent cell lines . Arch Otolaryngol Head Neck Surg 1981;107:703-710.Crossref 3. Fast LD, Hansen JA, Newman W: Evidence for T cell nature and heterogeneity within natural killer (NK) and antibody-dependent cellular cytotoxicity (ADCC) effectors: A comparison with cytolytic T lymphocytes (CTL) . J Immunol 1981;127:448-452. 4. Kay HD, Bonnard GD, West WH, et al: A functional comparison of human Fc-receptor bearing lymphocytes active in natural cytotoxicity and antibody-dependent cellular cytotoxicity . J Immunol 1977;118:2058-2066. 5. Zielske JV, Golub SH: Fetal calf seruminduced blastogenesis and cytotoxic responses of human lymphocytes . Cancer Res 1976;36:3842-3846. 6. Schiltz JR, Michel B: Production of epidermal acantholysis in normal human skin in vitro by the IgG fraction from pemphigus serum . J Invest Dermatol 1976;67:254-260.Crossref 7. Stanley JR, Koulu L, Thivolet C: Distinction between epidermal antigens binding pemphigus vulgaris and pemphigus foliaceous autoantibodies . J Clin Invest 1984;74:313-320.Crossref 8. Stanley JR, Koulu L, Klaus-Kovton V, et al: A monoclonal antibody to the desmosomal glycoprotein desmoglein I binds the same polypeptide as human autoantibodies in pemphigus foliaceous . J Immunol 1986;136:1227-1230. 9. Kipps TJ, Parham P, Punt J, et al: Importance of immunoglobulin isotype in human antibody-dependent cell-mediated cytotoxicity directed by murine monoclonal antibodies . J Exp Med 1985;161:1-17.Crossref 10. Christiaansen JE, Burnside SS, Sears DW: Apparent sensitivity of human K lymphocytes to the spatial orientation and organization of target cell-bound antibodies as measured by the efficiency of antibody-dependent cellular cytotoxicity (ADCC) . J Immunol 1987;138:2236-2243. 11. Shiloni E, Eisenthal A, Sachs D, et al: Antibody-dependent cellular cytotoxicity mediated by murine lymphocytes activated in recombinant interleukin 2 . J Immunol 1987;138:1992-1998. 12. Ortaldo JR, Woodhouse C, Morgan AC, et al: Analysis of effector cells in human antibody-dependent cellular cytotoxicity with murine monoclonal antibodies . J Immunol 1987;138:3566-3572. 13. Slezak S, Symer DE, Shin HS: Platelet-mediated cytotoxicity: Role of antibody and C3, and localization of the cytotoxic system in membranes . J Exp Med 1987;166:489-505.Crossref 14. Herlyn D, Koprowski H: IgG2a monoclonal antibodies inhibit human tumor growth through interaction with effector cells . Proc Natl Acad Sci USA 1982;79:4761-4765.Crossref 15. Berlinger NT, Hilal EY, Oettgen HF, et al: Deficient cell-mediated immunity in head and neck cancer patients secondary to autologous immune cells . Laryngoscope 1978;88:470-481.Crossref 16. Alexander E, Henkart P: The adherence of human Fc receptor–bearing lymphocytes to antigen-antibody complexes: II. Morphologic alterations induced by the substrate . J Exp Med 1976;143:329-347.Crossref 17. Simeone CB, Henkart P: Permeability changes induced in erythrocyte ghost targets by antibody-dependent cytotoxic effector cells: Evidence for membrane pores . J Immunol 1980; 124:954-963. 18. Malavasi F, Tetta C, Funaro A, et al: Fc receptor triggering induces expression of surface antigens and the release of platelet-activating factor in large granular lymphocytes . Proc Natl Acad Sci USA 1986;83:2443-2447.Crossref 19. Kondo LL, Rosenau W, Wora DW: Role of lymphotoxin in antibody-dependent cell-mediated cytotoxicity (ADCC) . J Immunol 1981; 126:1131-1133. 20. Duke RC, Chervenak R, Cohen JJ: Endogenous endonuclease-induced DNA fragmentation: An early event in cell-mediated lysis . Proc Natl Acad Sci USA 1983;80:6361-6365.Crossref 21. Masui H, Moroyama T, Mendelsohn J: Mechanism of antitumor activity in mice for anti-epidermal growth factor receptor monoclonal antibodies with different isotypes . Cancer Res 1986;46:5992-5998. 22. Herlyn D, Herlyn M, Steplewski Z, et al: Monoclonal anti-human tumor antibodies of six isotypes in cytotoxic reactions with human and murine effector cells . Cell Immunol 1985;92:105-114.Crossref 23. Weiner LM, Steplewski Z, Koprowski H, et al: Biological effects of gamma interferon pretreatment followed by monoclonal antibody 17-1A administration in patients with gastrointestinal carcinoma . Hybridoma 1986;5( (suppl) ):s65-s77. 24. Houghton AN, Mintzer D, Cordon-Cardo C, et al: Mouse monoclonal IgG3 antibody detecting GD3 ganglioside: A phase I trail in patients with malignant melanoma . Proc Natl Acad Sci USA 1985;82:1242-1246.Crossref 25. Sears HF, Mattis J, Herlyn D, et al: Phase-I clinical trial of monoclonal antibody in treatment of gastrointestinal tumors . Lancet 1982; 1:762-765.Crossref 26. Carey TE, Kimmel KA, Schwartz DR, et al: Antibodies to human squamous cell carcinoma . Otolaryngol Head Neck Surg 1983;91:482-491. 27. Koneval T, Applebaum E, Popovic D, et al: Demonstration of immunoglobulin in tumor and marginal tissues of squamous cell carcinomas of the head and neck . JNCI 1977;59:1089-1093. 28. Wolf GT, Hudson JL, Peterson KA, et al: Lymphocyte subpopulations infiltrating squamous carcinoma of the head and neck: Correlations with extent of tumor and prognosis . Otolaryngol Head Neck Surg 1986;95:142-152. 29. Danielson JR, Franklin WA: Characterization of antibody-forming cells adjacent to laryngeal carcinoma . Arch Otolaryngol Head Neck Surg 1984;110:327-328.Crossref 30. Wanebo HJ: Immunobiology of head and neck cancer: Basic concepts . Head Neck Surg 1979;2:42-55.Crossref 31. Mathew GD, Qualtiere LF, Neel HB, et al: Immunoglobulin A antibody to Epstein-Barr viral antigens and prognosis in nasopharyngeal carcinoma . Otolaryngol Head Neck Surg 1980;88:52-57. 32. Troye M, Hanssen Y, Paulie S, et al: Lymphocyte-mediated lysis of tumor cells in vitro (ADCC) induced by serum antibodies from patients with urinary bladder carcinoma or from controls . Int J Cancer 1980;25:45-51.Crossref 33. Heo DS, Whiteside TL, Johnson JT, et al: Long-term interleukin 2-dependent growth and cytotoxicity activity of tumor infiltrating lymphocytes from human squamous cell carcinomas of the head and neck . Cancer Res 1987;47:6353-6362. 34. Ariyan S, Krizek TJ, Mitchell MS: Identification of squamous cell carcinoma of the head and neck by tissue culture and immunological testing . Plast Reconstr Surg 1977;59:386-394.Crossref 35. Aust JC, Rabuzzi D, Reed G: Tissuecultured head and neck tumors: Their use in vitro assays of immune response . Trans Am Acad Ophthalmol Otolaryngol 1977;84:603-608.

Journal

Archives of Otolaryngology - Head & Neck SurgeryAmerican Medical Association

Published: Jun 1, 1989

References