Abstract Blood volume studies were performed in 18 healthy men and 564 male patients with a variety of clinical diagnoses. Although the plasma volume measurements obtained with iodinated I 125 serum albumin were accurate in patients with erythrocytosis and in healthy patients, this method overestimated the plasma volume in patients with traumatic injuries, carcinoma, cardiopulmonary disorders, and miscellaneous diagnoses. However, in 47 patients with traumatic injuries, carcinoma, and miscellaneous diagnoses, cold agglutinin labeled with radioactive iodine (125I) gave an accurate measurement of plasma volume. These data demonstrate the limitations of the iodinated albumin molecule in measuring plasma volume and in indirectly estimating red blood cell (RBC) volume from the total body hematocrit value and the plasma volume as determined with iodinated I 125 serum albumin. The best method was by determining red blood cell volume with radioactive chromium (51Cr)-labeled RBC, and estimating plasma volume. References 1. Fudenberg H, et al: The body hematocrit/venous hematocrit ratio and the "splenic reservoir." Blood 17:71-82, 1961. 2. Valeri CR: Effect of resuspension medium on the in vivo survival and supernatant hemoglobin of erythrocytes preserved with glycerol. Transfusion 5:25-35, 1965.Crossref 3. Button ML, Gibson JG II, Walter CW: Simultaneous determination of the volume of red cells and plasma for survival studies of stored blood. Transfusion 5:143-148, 1965.Crossref 4. Valeri CR, Szymanski IO, Runck AH: Therapeutic effectiveness of homologous erythrocyte transfusions following frozen storage at —80 C for up to seven years. Transfusion 10:102-112, 1970.Crossref 5. Strumia MM, Colwell LS, Dugan A: The measure of erythropoiesis in anemias: I. The mixing time and the immediate posttransfusion disappearance of T-1824 dye and of 51Cr-tagged erythrocytes in relation to blood volume determination. Blood 13:128-145, 1958. 6. Du bois D, Du bois EF: Clinical calorimetry: A formula to estimate the appropriate surface area if height and weight be known. Arch Intern Med 17:863-871, 1916.Crossref 7. Valeri CR: Observations on the chromium labeling of ACD-stored and previously frozen red cells. Transfusion 8:210-219, 1968.Crossref 8. Jones AR: A device for rapidly deriving the hematocrit of blood centrifuged in ungraduated tubes. N Engl J Med 254:172-174, 1956.Crossref 9. Miale A Jr: Measurements of Radioiodinated Albumin Metabolism in Man , in Sunderman FW, Sunderman FW Jr (eds): Serum Proteins and the Dysproteinemias . Philadelphia, JB Lippincott Co, 1964, vol 31, pp 307-319. 10. Wickerhauser M, Glaser C, Westcott W: Large-scale procedure for reprocessing outdated clinical albumin. Transfusion 10:264-271, 1970.Crossref 11. McFarlane AS: The behavior of 131I labeled plasma protein in vivo. Ann NY Acad Sci 70:19-25, 1957.Crossref 12. Steinfeld JL, et al: Degradation of iodinated human serum albumin prepared by various procedures. J Lab Clin Med 51:756-766, 1958. 13. Cooper AG: Purification of cold agglutinins from patients with chronic cold haemagglutinin disease. Clin Exp Immunol 3:691-702, 1968. 14. McFarlane AS: Labelling of plasma proteins with radioactive iodine. Biochem J 62:135-143, 1956. 15. McFarlane AS: The preparation of 131I and 125I labeled plasma proteins , Radioisotope Techniques in the Study of Protein Metabolism . Vienna, International Atomic Energy Agency, No. (45) , pp 3-6,1965. 16. Hays WL: Statistics for Psychologists . New York, Holt Rinehart & Winston, pp 333-335, 1963. 17. Siggaard-Andersen J, et al: Plasma volume and vascular permeability during hypoxia and carbon monoxide exposure. Scand J Clin Lab Invest 103( (suppl 22) ):39-48, 1968.Crossref 18. Strumia MM, Strumia PV, Dugan A: Significance of measurement of plasma volume and indirect estimation of red cell volume. Transfusion 8:197-209, 1968.Crossref 19. Mayerson HS, et al: Comparison of results of measurement of red cell volume by direct and indirect technics. Am J Physiol 155:232-238, 1948.
Archives of Internal Medicine – American Medical Association
Published: Oct 1, 1973