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Leukocyte Migration Inhibition Assay (LIF) in Nickel Contact Dermatitis

Leukocyte Migration Inhibition Assay (LIF) in Nickel Contact Dermatitis Abstract • A direct assay using the mixed leukocyte migration inhibition technique for leukocyte inhibition factor (LIF) production has shown that nickel-sensitive subjects can be distinguished from nonallergic controls. The difference between the two groups' migrating indexes was obtained with nickel sulfate in 199 medium at a concentration of 150 μg/ml and without prior coupling to a carrier protein. A concise review of the principal in vitro assays for cell-mediated immunity is included. (Arch Dermatol 112:1741-1744, 1976) References 1. Rich AR, Lewis MR: The nature of allergy in tuberculosis as revealed by tissue culture studies . Bull Johns Hopkins Hosp 50:115-131, 1932. 2. George M, Vaughan JH: In-vitro cell migration as a model for delayed hypersensitivity . Proc Soc Exp Biol Med 111:514-521, 1962.Crossref 3. Thor DE, et al: Cell migration inhibition factor released by antigen from human peripheral lymphocytes . Nature 219:755-757, 1968.Crossref 4. Rocklin RE, Meyers OL, David JR: An invitro assay for cellular hypersensitivity in man . J Immunol 104:95-102, 1970. 5. Dumonde DC, et al: "Lymphokines": Nonantibody mediators of cellular immunity generated by lymphocyte activation . Nature 224:38-42, 1969.Crossref 6. David JR: Lymphocyte mediators and cellular hypersensitivity . N Engl J Med 288:143-149, 1973.Crossref 7. Soborg M, Bendixen G: Human lymphocyte migration as a parameter of hypersensitivity . Acta Med Scand 181:247-256, 1967.Crossref 8. Soborg M: In-vitro detection of cellular hypersensitivity in man: Specific migration inhibition of white blood cells from brucella-positive persons . Acta Med Scand 182:167-174, 1967.Crossref 9. Mills JA: The immunologic significance of antigen induced lymphocyte transformation invitro . J Immunol 97:239-247, 1966. 10. Milner JE: In-vitro lymphocyte responses in contact hypersensitivity . J Invest Dermatol 55:34-38, 1970.Crossref 11. Rocklin RE, et al: Dissociation between two in-vitro correlates of delayed hypersensitivity: Absence of migration inhibitory factor (MIF) in the presence of antigen-induced incorporation of 3 H-thymidine , in Harris JE (ed): Proceedings of the Fifth Leukocyte Culture Conferences . New York, Academic Press Inc, 1970, pp 639-648. 12. Lazarus GS, et al: Pyoderma gangrenosum: Altered delayed hypersensitivity and polyarthritis . Arch Dermatol 105:46-51, 1972.Crossref 13. Parkhouse RM: Antigen-stimulated DNA and RNA synthesis in spleen cell suspensions from immunized rabbits . Nature 215:394-395, 1967.Crossref 14. Levis WR, Miller AE Jr: Lymphocyte transformation during DNCB contact sensitization . Fed Proc 32:956, 1973. 15. Saurat JH, et al: Cell-mediated hypersensitivity in skin reactions to drugs (except contact dermatitis) . Clin Allergy 3:427-437, 1973.Crossref 16. Kaltreider HY, et al: Capillary tube migration for detection of human delayed hypersensitivity: Difficulties encountered in "buffy coat" cells and tuberculin antigen . J Immunol 103:179-184, 1969. 17. Lockshin MD: Failure to demonstrate leukocyte migration inhibition in human tuberculin hypersensitivity . Proc Soc Exp Biol Med 132:928-930, 1969.Crossref 18. Rosenberg SA, David JR: Inhibition of leukocyte migration: An evaluation of this invitro assay of delayed hypersensitivity in man to a soluble antigen . J Immunol 105:1447-1452, 1970. 19. Mookerjee B, Ackman CFD, Dossetor JB: Delayed hypersensitivity in-vitro using human peripheral leukocytes . Transplantation 8:745-748, 1969.Crossref 20. Wolfson RL, Maddison SE, Kaggan IG: Migration inhibition of peripheral leukocytes in human schistosomiasis . J Immunol 109:123-128, 1972. 21. Budtz-Jorgensen E: Delayed hypersensitivity to Candida albicans in man: Demonstration in-vitro: The capillary tube migration test . Acta Allergol (Kbh) 27:41-49, 1972.Crossref 22. Nerup J, Andersen V, Bendixen G: Antiadrenal cellular hypersensitivity in Addison's disease: IV. In-vivo and in-vitro investigations on the mitochondrial fraction . Clin Exp Immunol 6:733-739, 1970. 23. Bendixen G: Organ specific inhibition of the in vitro migration of leukocytes in human glomerulonephritis . Acta Med Scand 184:99-103, 1968.Crossref 24. Brostoff J: Critique of present in-vitro methods for the detection of cell-mediated immunity . Proc R Soc Med 67:514-516, 1974. 25. Thulin H, Zachariae H: The leukocyte migration test in chromium hypersensitivity . J Invest Dermatol 58:55-58, 1972.Crossref 26. Czernielewski A, Libiscowski T, Dudek H: Use of the leukocyte migration inhibition test (MIF) in the detection of hypersensitivity to chromium compounds: I. Combination of human albumin with chromium as an antigen in MIF . Przegl Dermatol 61:797-802, 1974. 27. Nordqvist B, Rorsman H: Leukcocytic migration in-vitro as an indicator of allergy in eczematous contact dermatitis . Trans St Johns Hosp Dermatol Soc 53:154-159, 1967. 28. Jordan WP, Higgins MR, Dvorak J: Allergic contact dermatitis to all-transretinoic acid: Epicutaneous and leukocyte migration inhibition testing . Contact Dermatitis 1:306-310, 1975.Crossref 29. Mirza M, Perera MG, Bernstein IL: Leukocyte migration inhibition in nickel dermatitis . Fed Proc 33:728, 1974. 30. Friend JV, Lane M: In-vitro studies of contact hypersensitivity: The effect of the haptenes 2, 4-dinitrochlorobenzene (DNCB) and 2,4-dinitrofluorobenzene (DNFB) and of haptene protein conjugates on the migration of guinea pig peritoneal exudate cells . Immunology 25:869-874, 1973. 31. Herman PS, Sams WM Jr: Requirement for carrier protein in salicylanilide sensitivity: The migration-inhibition test in contact photoallergy . J Lab Clin Med 77:572-579, 1971. 32. Gimenez-Camarasa JM, et al: Lymphocyte transformation test in allergic contact nickel dermatitis . Br J Dermatol 92:9-15, 1975.Crossref 33. Millikan LE, Conway F, Foote JE: In-vitro studies of contact hypersensitivity: Lymphocyte transformation in nickel sensitivity . J Invest Dermatol 60:88-90, 1973.Crossref 34. Milner JE: In-vitro lymphocyte responses in contact hypersensitivity IV . J Invest Dermatol 62:591-594, 1974.Crossref 35. Miller AE Jr, Levis WR: In-vitro studies of allergic contact dermatitis to DNCB , abstracted. J Invest Dermatol 60:105, 1973. 36. Maini RN, et al: Standardization of the leukocyte migration test . Int Arch Allergy Appl Immunol 45:308-321, 1973.Crossref 37. Fimmel PJ, Keast D: Studies on in-vitro migration inhibition . Aust J Exp Biol Med Sci 52:745-753, 1974.Crossref 38. Federlin K, et al: A micro-method for peripheral leukocyte migration in tuberculin sensitivity . J Clin Pathol 24:533-536, 1971.Crossref 39. Rocklin RE: Products of activated lymphocytes: Leukocyte inhibitory factor (LIF) distinct from migratory inhibitory factor (MIF) . J Immunol 112:1461-1466, 1974. 40. Rocklin RE: Partial characterization of leukocyte inhibitory factor by concanavalin A-stimulated human lymphocytes (LIFcon A) . J Immunol 114:1161-1165, 1975. 41. Fimmel PJ: Studies on leukocyte migration inhibition: The role of E rosette forming cells in specific antigen-induced inhibition of migration . J Immunol 115:135-138, 1975. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Leukocyte Migration Inhibition Assay (LIF) in Nickel Contact Dermatitis

Archives of Dermatology , Volume 112 (12) – Dec 1, 1976

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Publisher
American Medical Association
Copyright
Copyright © 1976 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1976.01630370029006
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Abstract

Abstract • A direct assay using the mixed leukocyte migration inhibition technique for leukocyte inhibition factor (LIF) production has shown that nickel-sensitive subjects can be distinguished from nonallergic controls. The difference between the two groups' migrating indexes was obtained with nickel sulfate in 199 medium at a concentration of 150 μg/ml and without prior coupling to a carrier protein. A concise review of the principal in vitro assays for cell-mediated immunity is included. (Arch Dermatol 112:1741-1744, 1976) References 1. Rich AR, Lewis MR: The nature of allergy in tuberculosis as revealed by tissue culture studies . Bull Johns Hopkins Hosp 50:115-131, 1932. 2. George M, Vaughan JH: In-vitro cell migration as a model for delayed hypersensitivity . Proc Soc Exp Biol Med 111:514-521, 1962.Crossref 3. Thor DE, et al: Cell migration inhibition factor released by antigen from human peripheral lymphocytes . Nature 219:755-757, 1968.Crossref 4. Rocklin RE, Meyers OL, David JR: An invitro assay for cellular hypersensitivity in man . J Immunol 104:95-102, 1970. 5. Dumonde DC, et al: "Lymphokines": Nonantibody mediators of cellular immunity generated by lymphocyte activation . Nature 224:38-42, 1969.Crossref 6. David JR: Lymphocyte mediators and cellular hypersensitivity . N Engl J Med 288:143-149, 1973.Crossref 7. Soborg M, Bendixen G: Human lymphocyte migration as a parameter of hypersensitivity . Acta Med Scand 181:247-256, 1967.Crossref 8. Soborg M: In-vitro detection of cellular hypersensitivity in man: Specific migration inhibition of white blood cells from brucella-positive persons . Acta Med Scand 182:167-174, 1967.Crossref 9. Mills JA: The immunologic significance of antigen induced lymphocyte transformation invitro . J Immunol 97:239-247, 1966. 10. Milner JE: In-vitro lymphocyte responses in contact hypersensitivity . J Invest Dermatol 55:34-38, 1970.Crossref 11. Rocklin RE, et al: Dissociation between two in-vitro correlates of delayed hypersensitivity: Absence of migration inhibitory factor (MIF) in the presence of antigen-induced incorporation of 3 H-thymidine , in Harris JE (ed): Proceedings of the Fifth Leukocyte Culture Conferences . New York, Academic Press Inc, 1970, pp 639-648. 12. Lazarus GS, et al: Pyoderma gangrenosum: Altered delayed hypersensitivity and polyarthritis . Arch Dermatol 105:46-51, 1972.Crossref 13. Parkhouse RM: Antigen-stimulated DNA and RNA synthesis in spleen cell suspensions from immunized rabbits . Nature 215:394-395, 1967.Crossref 14. Levis WR, Miller AE Jr: Lymphocyte transformation during DNCB contact sensitization . Fed Proc 32:956, 1973. 15. Saurat JH, et al: Cell-mediated hypersensitivity in skin reactions to drugs (except contact dermatitis) . Clin Allergy 3:427-437, 1973.Crossref 16. Kaltreider HY, et al: Capillary tube migration for detection of human delayed hypersensitivity: Difficulties encountered in "buffy coat" cells and tuberculin antigen . J Immunol 103:179-184, 1969. 17. Lockshin MD: Failure to demonstrate leukocyte migration inhibition in human tuberculin hypersensitivity . Proc Soc Exp Biol Med 132:928-930, 1969.Crossref 18. Rosenberg SA, David JR: Inhibition of leukocyte migration: An evaluation of this invitro assay of delayed hypersensitivity in man to a soluble antigen . J Immunol 105:1447-1452, 1970. 19. Mookerjee B, Ackman CFD, Dossetor JB: Delayed hypersensitivity in-vitro using human peripheral leukocytes . Transplantation 8:745-748, 1969.Crossref 20. Wolfson RL, Maddison SE, Kaggan IG: Migration inhibition of peripheral leukocytes in human schistosomiasis . J Immunol 109:123-128, 1972. 21. Budtz-Jorgensen E: Delayed hypersensitivity to Candida albicans in man: Demonstration in-vitro: The capillary tube migration test . Acta Allergol (Kbh) 27:41-49, 1972.Crossref 22. Nerup J, Andersen V, Bendixen G: Antiadrenal cellular hypersensitivity in Addison's disease: IV. In-vivo and in-vitro investigations on the mitochondrial fraction . Clin Exp Immunol 6:733-739, 1970. 23. Bendixen G: Organ specific inhibition of the in vitro migration of leukocytes in human glomerulonephritis . Acta Med Scand 184:99-103, 1968.Crossref 24. Brostoff J: Critique of present in-vitro methods for the detection of cell-mediated immunity . Proc R Soc Med 67:514-516, 1974. 25. Thulin H, Zachariae H: The leukocyte migration test in chromium hypersensitivity . J Invest Dermatol 58:55-58, 1972.Crossref 26. Czernielewski A, Libiscowski T, Dudek H: Use of the leukocyte migration inhibition test (MIF) in the detection of hypersensitivity to chromium compounds: I. Combination of human albumin with chromium as an antigen in MIF . Przegl Dermatol 61:797-802, 1974. 27. Nordqvist B, Rorsman H: Leukcocytic migration in-vitro as an indicator of allergy in eczematous contact dermatitis . Trans St Johns Hosp Dermatol Soc 53:154-159, 1967. 28. Jordan WP, Higgins MR, Dvorak J: Allergic contact dermatitis to all-transretinoic acid: Epicutaneous and leukocyte migration inhibition testing . Contact Dermatitis 1:306-310, 1975.Crossref 29. Mirza M, Perera MG, Bernstein IL: Leukocyte migration inhibition in nickel dermatitis . Fed Proc 33:728, 1974. 30. Friend JV, Lane M: In-vitro studies of contact hypersensitivity: The effect of the haptenes 2, 4-dinitrochlorobenzene (DNCB) and 2,4-dinitrofluorobenzene (DNFB) and of haptene protein conjugates on the migration of guinea pig peritoneal exudate cells . Immunology 25:869-874, 1973. 31. Herman PS, Sams WM Jr: Requirement for carrier protein in salicylanilide sensitivity: The migration-inhibition test in contact photoallergy . J Lab Clin Med 77:572-579, 1971. 32. Gimenez-Camarasa JM, et al: Lymphocyte transformation test in allergic contact nickel dermatitis . Br J Dermatol 92:9-15, 1975.Crossref 33. Millikan LE, Conway F, Foote JE: In-vitro studies of contact hypersensitivity: Lymphocyte transformation in nickel sensitivity . J Invest Dermatol 60:88-90, 1973.Crossref 34. Milner JE: In-vitro lymphocyte responses in contact hypersensitivity IV . J Invest Dermatol 62:591-594, 1974.Crossref 35. Miller AE Jr, Levis WR: In-vitro studies of allergic contact dermatitis to DNCB , abstracted. J Invest Dermatol 60:105, 1973. 36. Maini RN, et al: Standardization of the leukocyte migration test . Int Arch Allergy Appl Immunol 45:308-321, 1973.Crossref 37. Fimmel PJ, Keast D: Studies on in-vitro migration inhibition . Aust J Exp Biol Med Sci 52:745-753, 1974.Crossref 38. Federlin K, et al: A micro-method for peripheral leukocyte migration in tuberculin sensitivity . J Clin Pathol 24:533-536, 1971.Crossref 39. Rocklin RE: Products of activated lymphocytes: Leukocyte inhibitory factor (LIF) distinct from migratory inhibitory factor (MIF) . J Immunol 112:1461-1466, 1974. 40. Rocklin RE: Partial characterization of leukocyte inhibitory factor by concanavalin A-stimulated human lymphocytes (LIFcon A) . J Immunol 114:1161-1165, 1975. 41. Fimmel PJ: Studies on leukocyte migration inhibition: The role of E rosette forming cells in specific antigen-induced inhibition of migration . J Immunol 115:135-138, 1975.

Journal

Archives of DermatologyAmerican Medical Association

Published: Dec 1, 1976

References