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Lens Antigens in Ocular Tissues: An Immunoelectrophoretic Study

Lens Antigens in Ocular Tissues: An Immunoelectrophoretic Study Abstract Introduction Antigens identical immunologically to those of the lens have been demonstrated in the cornea, iris, and pigment retina of the chick eye (Langman and Prescott1; Maisel and Langman2). Furthermore, these tissues have the ability to form a lens upon removal of the original lens during embryonic life as well as in the newborn chick (Van Deth3). It has therefore been suggested that lens antigens present in these tissues may provide the biochemical substrate for lens regeneration.1,2 Further support for this hypothesis would be provided if it could be shown that the structure of these extralenticular proteins also resembles that of the lens proteins with respect to electrophoretic and molecular weight properties.The immunological techniques which have hitherto been used for the detection of such common antigens give information only regarding their determinate groups. The present investigation was therefore undertaken in order to compare the electrophoretic References 1. Langman, J., and Prescott, B. D.: An Immunological Approach to the Problem of Lens Regeneration , J. Embryol. Exp. Morph. 7:549, 1959. 2. Maisel, H., and Langman, J.: Lens Proteins in Various Tissues of the Chick Eye and in the Lens of Animals Throughout the Vertebrate Series , Anat. Rec. 140:183, 1961.Crossref 3. Van Deth, J. H. M. G.: Induction et regeneration du Cristallin chez l'embryon de la poule , Acta Morph. Neerl. (Scand.) 3:151, 1940. 4. Williams, G. A., Jr. and, Grabar, P.: Immunoelectrophoretic Studies in Serum Proteins , J. Immun. 74:158, 1955. 5. Wadsworth, C., and Hanson, L. A.: Comparative Analysis of Immune Electrophoretic Precipitates Employing a Modified Immune Electrophoretic Technique , Int. Arch. Allergy 17:165, 1960.Crossref 6. Maisel, H., and Langman, J.: An Immuno-Embryological Study on the Chick Lens , J. Embryol. Exp. Morph. 9:191, 1961. 7. François, J.; Rabaey, M.; Wieme, J. R., and Kaminski, M.: Study of the Antigens of the Crystalline Lens , Amer. J. Ophthal. 42:577, 1956. 8. Clayton, R. M.: Localization of Embryonic Antigens by Antisera Labelled with Fluorescent Dyes , Nature 174:1059, 1954.Crossref 9. Waddington, C. H., and Cohen, A.: Experiments on the Development of the Head of the Chick Embryo , J. Exp. Biol. 13:2191936. 10. Mangold, O.: Das Determinationsproblem: III. Das Wirbeltierauge in der Entwicklung und Regeneration , Ergebn. Biol. 7:193, 1931. 11. Fischel, A.: Über den Einfluss des Auges auf die Entwicklung und Erhaltung der Hornhaut , Klin. Mbl. Augenheilk. 62:1, 1919. 12. Uhlenhuth, P. T.: Zur Lehre von der Unterscheidung verschiedener Eiweissarten mit Hilfe spezifischer Sera , Robert Koch Festschrift, Jena, Gustav Fisher, 1903 p. 49. 13. Halbert S. P., and FitzGerald, P. L.: Studies on the Immunologic Organ Specificity of Ocular Lens , Amer. J. Ophthal. 46:187, (Nov., (Pt. 2) ) 1958. 14. Manski, W.; Auerbach, T. P., and Halbert, S. P.: The Evolutionary Significance of Lens Organ Specificity , Amer. J. Ophthal. 50:985 (Nov., (Pt. 2) ) 1960. 15. Flickinger, R. A., and Stone, G.: Localization of Lens Antigens in Developing Frog Embryos , Exp. Cell Res. 21:541, 1960.Crossref 16. Rao, S. S.; Kulkarni, M. E.; Cooper, S. M., and Radhakrishnan, M. R.: Analysis of Proteins of Bovine Lens Vitreous and Aqueous by Electrophoresis and by Oudin's Gel Diffusion Technique , Brit. J. Ophthal. 39:163, 1955.Crossref 17. Woods, A. C.: The Diagnosis and Treatment of Ocular Allergy , Amer. J. Ophthal. 32:1457, 1949. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Lens Antigens in Ocular Tissues: An Immunoelectrophoretic Study

Archives of Ophthalmology , Volume 68 (2) – Aug 1, 1962

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Publisher
American Medical Association
Copyright
Copyright © 1962 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1962.00960030258018
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Abstract

Abstract Introduction Antigens identical immunologically to those of the lens have been demonstrated in the cornea, iris, and pigment retina of the chick eye (Langman and Prescott1; Maisel and Langman2). Furthermore, these tissues have the ability to form a lens upon removal of the original lens during embryonic life as well as in the newborn chick (Van Deth3). It has therefore been suggested that lens antigens present in these tissues may provide the biochemical substrate for lens regeneration.1,2 Further support for this hypothesis would be provided if it could be shown that the structure of these extralenticular proteins also resembles that of the lens proteins with respect to electrophoretic and molecular weight properties.The immunological techniques which have hitherto been used for the detection of such common antigens give information only regarding their determinate groups. The present investigation was therefore undertaken in order to compare the electrophoretic References 1. Langman, J., and Prescott, B. D.: An Immunological Approach to the Problem of Lens Regeneration , J. Embryol. Exp. Morph. 7:549, 1959. 2. Maisel, H., and Langman, J.: Lens Proteins in Various Tissues of the Chick Eye and in the Lens of Animals Throughout the Vertebrate Series , Anat. Rec. 140:183, 1961.Crossref 3. Van Deth, J. H. M. G.: Induction et regeneration du Cristallin chez l'embryon de la poule , Acta Morph. Neerl. (Scand.) 3:151, 1940. 4. Williams, G. A., Jr. and, Grabar, P.: Immunoelectrophoretic Studies in Serum Proteins , J. Immun. 74:158, 1955. 5. Wadsworth, C., and Hanson, L. A.: Comparative Analysis of Immune Electrophoretic Precipitates Employing a Modified Immune Electrophoretic Technique , Int. Arch. Allergy 17:165, 1960.Crossref 6. Maisel, H., and Langman, J.: An Immuno-Embryological Study on the Chick Lens , J. Embryol. Exp. Morph. 9:191, 1961. 7. François, J.; Rabaey, M.; Wieme, J. R., and Kaminski, M.: Study of the Antigens of the Crystalline Lens , Amer. J. Ophthal. 42:577, 1956. 8. Clayton, R. M.: Localization of Embryonic Antigens by Antisera Labelled with Fluorescent Dyes , Nature 174:1059, 1954.Crossref 9. Waddington, C. H., and Cohen, A.: Experiments on the Development of the Head of the Chick Embryo , J. Exp. Biol. 13:2191936. 10. Mangold, O.: Das Determinationsproblem: III. Das Wirbeltierauge in der Entwicklung und Regeneration , Ergebn. Biol. 7:193, 1931. 11. Fischel, A.: Über den Einfluss des Auges auf die Entwicklung und Erhaltung der Hornhaut , Klin. Mbl. Augenheilk. 62:1, 1919. 12. Uhlenhuth, P. T.: Zur Lehre von der Unterscheidung verschiedener Eiweissarten mit Hilfe spezifischer Sera , Robert Koch Festschrift, Jena, Gustav Fisher, 1903 p. 49. 13. Halbert S. P., and FitzGerald, P. L.: Studies on the Immunologic Organ Specificity of Ocular Lens , Amer. J. Ophthal. 46:187, (Nov., (Pt. 2) ) 1958. 14. Manski, W.; Auerbach, T. P., and Halbert, S. P.: The Evolutionary Significance of Lens Organ Specificity , Amer. J. Ophthal. 50:985 (Nov., (Pt. 2) ) 1960. 15. Flickinger, R. A., and Stone, G.: Localization of Lens Antigens in Developing Frog Embryos , Exp. Cell Res. 21:541, 1960.Crossref 16. Rao, S. S.; Kulkarni, M. E.; Cooper, S. M., and Radhakrishnan, M. R.: Analysis of Proteins of Bovine Lens Vitreous and Aqueous by Electrophoresis and by Oudin's Gel Diffusion Technique , Brit. J. Ophthal. 39:163, 1955.Crossref 17. Woods, A. C.: The Diagnosis and Treatment of Ocular Allergy , Amer. J. Ophthal. 32:1457, 1949.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Aug 1, 1962

References