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Knife Wetting Device: Wet Celloidin Technique

Knife Wetting Device: Wet Celloidin Technique Abstract IN preparing histological sections using the wet celloidin technique the knife of the sliding microtone, the block being cut, and the sections cut from it must be kept wet with 80% alcohol. The block is kept wet with a steady drip from a dropping bottle. Usually the knife and the sections are kept wet by frequent applications of alcohol with a camels hair brush, which is a time-consuming procedure. The device described here eliminates the brush procedure by providing a steady controlled flow of alcohol to the knife surface, or any part of it desired, by siphon from a small plastic bottle mounted on the knife holder. For a sliding microtome with adjustable knife clamp, the device shown in the Figure includes: a plastic bottle, 130 ml capacity (A); a bottle holder made from an L-shaped piece of sheet aluminum bent to fit around and under the bottle—a hole in http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Otolaryngology American Medical Association

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Publisher
American Medical Association
Copyright
Copyright © 1968 American Medical Association. All Rights Reserved.
ISSN
0003-9977
DOI
10.1001/archotol.1968.00760060441021
Publisher site
See Article on Publisher Site

Abstract

Abstract IN preparing histological sections using the wet celloidin technique the knife of the sliding microtone, the block being cut, and the sections cut from it must be kept wet with 80% alcohol. The block is kept wet with a steady drip from a dropping bottle. Usually the knife and the sections are kept wet by frequent applications of alcohol with a camels hair brush, which is a time-consuming procedure. The device described here eliminates the brush procedure by providing a steady controlled flow of alcohol to the knife surface, or any part of it desired, by siphon from a small plastic bottle mounted on the knife holder. For a sliding microtome with adjustable knife clamp, the device shown in the Figure includes: a plastic bottle, 130 ml capacity (A); a bottle holder made from an L-shaped piece of sheet aluminum bent to fit around and under the bottle—a hole in

Journal

Archives of OtolaryngologyAmerican Medical Association

Published: Apr 1, 1968

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