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Kittens as Experimental Model for Study of Kernicterus

Kittens as Experimental Model for Study of Kernicterus Abstract IN AN EARLIER publication,1 a method was reported by which brain damage, similar to that of kernicteric infants, was produced in newborn kittens by intravenous injections of bilirubin. In subsequent work an attempt was made to develop a simple and reliable technique by which these animals could be used as an experimental tool for the further study of bilirubin toxicity in vivo. Such a method has been worked out and is presented in this communication. Materials and Method Newborn kittens in good health were used in this series of experiments. They received intravenous injections of bilirubin in a solution of bovine albumin. The solution was prepared according to the following formula.Crystalline bilirubin (Nutritional Biochemicals) 500 mg 0.75 N NaOH 5.0 ml Distilled water 5.0 ml Bovine albumin 30% (Armour) 5.0 ml 1M KH2PO4 2.75 mlIn this modification, we used double dilution of sodium hydroxide References 1. Rozdilsky, B., and Olszewski, J.: Experimental Study of the Toxicity of Bilirubin in Newborn Animals , J Neuropath Exp Neurol 20:193-205, 1961.Crossref 2. Johnson, L., et al: Kernicterus in Rats With an Inherited Deficiency of Glucuronyl Transferase , Amer J Dis Child 97:591-608, 1959. 3. Lucey, J.F., et al: Kernicterus in Asphyxiated Newborn Rhesus Monkeys , Exp Neurol 9:43-58, 1964.Crossref 4. Rozdilsky, B., and Olszewski, J.: Permeability of Cerebral Vessels to Albumin in Hyperbilirubinemia , Neurology 10:631-638, 1960.Crossref 5. Martin, N.H.: Preparation and Properties of Serum and Plasma Proteins: XXI. Interactions With Bilirubin , J Amer Chem Soc 71:1230-1232, 1949.Crossref 6. Odell, G.B.: Studies in Kernicterus: 1. The Protein Binding of Bilirubin . J Clin Invest 38: 823-833, 1959.Crossref 7. Johnson, L.M.: " The Effect of Certain Substances on Bilirubin Levels and Occurrence of Kernicterus in Genetically Jaundiced Rats ," in Sass-Kortsák, A. (ed.): Kernicterus: Report Based on a Symposium Held at the IX International Congress of Paediatrics, Montreal, July 1959 . Toronto: University of Toronto Press, 1961, pp 208-218. 8. Johnson L., et al: Kernicterus in Rats Lacking Glucuronyl Transferase , Amer J Dis Child 101:322-349, 1961.Crossref 9. Waters, W.J.: " The Protective Action of Albumin in Bilirubin Toxicity in Newborn Puppies ," in Sass-Kortsák, A. (ed.): Kernicterus—Report Based on a Symposium Held at the IX International Congress of Paediatrics, Montreal, July 1959 , Toronto: University of Toronto Press, 1961, pp 219-221. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Diseases of Children American Medical Association

Kittens as Experimental Model for Study of Kernicterus

American Journal of Diseases of Children , Volume 111 (2) – Feb 1, 1966

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Publisher
American Medical Association
Copyright
Copyright © 1966 American Medical Association. All Rights Reserved.
ISSN
0002-922X
DOI
10.1001/archpedi.1966.02090050093006
Publisher site
See Article on Publisher Site

Abstract

Abstract IN AN EARLIER publication,1 a method was reported by which brain damage, similar to that of kernicteric infants, was produced in newborn kittens by intravenous injections of bilirubin. In subsequent work an attempt was made to develop a simple and reliable technique by which these animals could be used as an experimental tool for the further study of bilirubin toxicity in vivo. Such a method has been worked out and is presented in this communication. Materials and Method Newborn kittens in good health were used in this series of experiments. They received intravenous injections of bilirubin in a solution of bovine albumin. The solution was prepared according to the following formula.Crystalline bilirubin (Nutritional Biochemicals) 500 mg 0.75 N NaOH 5.0 ml Distilled water 5.0 ml Bovine albumin 30% (Armour) 5.0 ml 1M KH2PO4 2.75 mlIn this modification, we used double dilution of sodium hydroxide References 1. Rozdilsky, B., and Olszewski, J.: Experimental Study of the Toxicity of Bilirubin in Newborn Animals , J Neuropath Exp Neurol 20:193-205, 1961.Crossref 2. Johnson, L., et al: Kernicterus in Rats With an Inherited Deficiency of Glucuronyl Transferase , Amer J Dis Child 97:591-608, 1959. 3. Lucey, J.F., et al: Kernicterus in Asphyxiated Newborn Rhesus Monkeys , Exp Neurol 9:43-58, 1964.Crossref 4. Rozdilsky, B., and Olszewski, J.: Permeability of Cerebral Vessels to Albumin in Hyperbilirubinemia , Neurology 10:631-638, 1960.Crossref 5. Martin, N.H.: Preparation and Properties of Serum and Plasma Proteins: XXI. Interactions With Bilirubin , J Amer Chem Soc 71:1230-1232, 1949.Crossref 6. Odell, G.B.: Studies in Kernicterus: 1. The Protein Binding of Bilirubin . J Clin Invest 38: 823-833, 1959.Crossref 7. Johnson, L.M.: " The Effect of Certain Substances on Bilirubin Levels and Occurrence of Kernicterus in Genetically Jaundiced Rats ," in Sass-Kortsák, A. (ed.): Kernicterus: Report Based on a Symposium Held at the IX International Congress of Paediatrics, Montreal, July 1959 . Toronto: University of Toronto Press, 1961, pp 208-218. 8. Johnson L., et al: Kernicterus in Rats Lacking Glucuronyl Transferase , Amer J Dis Child 101:322-349, 1961.Crossref 9. Waters, W.J.: " The Protective Action of Albumin in Bilirubin Toxicity in Newborn Puppies ," in Sass-Kortsák, A. (ed.): Kernicterus—Report Based on a Symposium Held at the IX International Congress of Paediatrics, Montreal, July 1959 , Toronto: University of Toronto Press, 1961, pp 219-221.

Journal

American Journal of Diseases of ChildrenAmerican Medical Association

Published: Feb 1, 1966

References