Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You and Your Team.

Learn More →

Insulin and Glucagon Responses of Transplanted Intrasplenic Pancreatic Islets

Insulin and Glucagon Responses of Transplanted Intrasplenic Pancreatic Islets Abstract • Two transplant procedures have been investigated in which one third of the pancreas was autotransplanted into the splenic pulp of dogs. The two procedures consist of simple mechanical dissociation of the pancreas or mechanical dissociation followed by collagenase digestion. The ability of the endocrine segment of the transplant to survive and function was assessed by stimulation with arginine and measurement of insulin and glucagon response. The results demonstrate that both transplant procedures result in functioning beta and alpha cells that rapidly secrete both insulin and glucagon in response to arginine stimulation. However, greater insulin responses were obtained when mechanically dissociated but nonenzyme digested pancreatic tissue was used for transplantation. The spleen appears to be an excellent transplant site for the reception of endocrine pancreatic tissue and allowed both beta and alpha cells to survive following transplantation. (Arch Surg 114:96-99, 1979) References 1. Mirkovitch V, Campiche M: Successful intrasplenic autotransplantation of pancreatic tissue in totally pancreatectomized dogs . Transplantation 21:265-269, 1976.Crossref 2. Kretschmer GJ, Sutherland D, Matas A, et al: Autotransplantation of pancreatic islets without separation of exo- and endocrine tissue in totally pancreatectomized dogs . Surgery 82:74, 1977. 3. Freychet P, Roth J, Neville DM: Monoiodoinsulin: Demonstration of its biological activity and binding to fat cells and liver membranes . Biochem Biophys Res Commun 43:400, 1971.Crossref 4. Albano JDM, Edkins RP, Maritz G, et al: A sensitive precise radioimmunoassay of serum insulin relying on charcoal separation of bound and free hormone moieties . Acta Endocrinol 70:487-509, 1972. 5. Alford F, Bloom SR, Nabarro JDN: Glucagon levels in normal and diabetic subjects: Use of a specific immuno-absorbent for glucagon radioimmunoassay . Diabetologia 13:1-6, 1977.Crossref 6. Jorgensen KH, Larsen UD: Purification of 125I-glucagon by anion exchange chromatography . Horm Metab Res 4:4223-4224, 1972.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

Insulin and Glucagon Responses of Transplanted Intrasplenic Pancreatic Islets

Loading next page...
 
/lp/american-medical-association/insulin-and-glucagon-responses-of-transplanted-intrasplenic-pancreatic-WXbNqFhUT0
Publisher
American Medical Association
Copyright
Copyright © 1979 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1979.01370250098022
Publisher site
See Article on Publisher Site

Abstract

Abstract • Two transplant procedures have been investigated in which one third of the pancreas was autotransplanted into the splenic pulp of dogs. The two procedures consist of simple mechanical dissociation of the pancreas or mechanical dissociation followed by collagenase digestion. The ability of the endocrine segment of the transplant to survive and function was assessed by stimulation with arginine and measurement of insulin and glucagon response. The results demonstrate that both transplant procedures result in functioning beta and alpha cells that rapidly secrete both insulin and glucagon in response to arginine stimulation. However, greater insulin responses were obtained when mechanically dissociated but nonenzyme digested pancreatic tissue was used for transplantation. The spleen appears to be an excellent transplant site for the reception of endocrine pancreatic tissue and allowed both beta and alpha cells to survive following transplantation. (Arch Surg 114:96-99, 1979) References 1. Mirkovitch V, Campiche M: Successful intrasplenic autotransplantation of pancreatic tissue in totally pancreatectomized dogs . Transplantation 21:265-269, 1976.Crossref 2. Kretschmer GJ, Sutherland D, Matas A, et al: Autotransplantation of pancreatic islets without separation of exo- and endocrine tissue in totally pancreatectomized dogs . Surgery 82:74, 1977. 3. Freychet P, Roth J, Neville DM: Monoiodoinsulin: Demonstration of its biological activity and binding to fat cells and liver membranes . Biochem Biophys Res Commun 43:400, 1971.Crossref 4. Albano JDM, Edkins RP, Maritz G, et al: A sensitive precise radioimmunoassay of serum insulin relying on charcoal separation of bound and free hormone moieties . Acta Endocrinol 70:487-509, 1972. 5. Alford F, Bloom SR, Nabarro JDN: Glucagon levels in normal and diabetic subjects: Use of a specific immuno-absorbent for glucagon radioimmunoassay . Diabetologia 13:1-6, 1977.Crossref 6. Jorgensen KH, Larsen UD: Purification of 125I-glucagon by anion exchange chromatography . Horm Metab Res 4:4223-4224, 1972.Crossref

Journal

Archives of SurgeryAmerican Medical Association

Published: Jan 1, 1979

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$499/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month