Abstract • Two transplant procedures have been investigated in which one third of the pancreas was autotransplanted into the splenic pulp of dogs. The two procedures consist of simple mechanical dissociation of the pancreas or mechanical dissociation followed by collagenase digestion. The ability of the endocrine segment of the transplant to survive and function was assessed by stimulation with arginine and measurement of insulin and glucagon response. The results demonstrate that both transplant procedures result in functioning beta and alpha cells that rapidly secrete both insulin and glucagon in response to arginine stimulation. However, greater insulin responses were obtained when mechanically dissociated but nonenzyme digested pancreatic tissue was used for transplantation. The spleen appears to be an excellent transplant site for the reception of endocrine pancreatic tissue and allowed both beta and alpha cells to survive following transplantation. (Arch Surg 114:96-99, 1979) References 1. Mirkovitch V, Campiche M: Successful intrasplenic autotransplantation of pancreatic tissue in totally pancreatectomized dogs . Transplantation 21:265-269, 1976.Crossref 2. Kretschmer GJ, Sutherland D, Matas A, et al: Autotransplantation of pancreatic islets without separation of exo- and endocrine tissue in totally pancreatectomized dogs . Surgery 82:74, 1977. 3. Freychet P, Roth J, Neville DM: Monoiodoinsulin: Demonstration of its biological activity and binding to fat cells and liver membranes . Biochem Biophys Res Commun 43:400, 1971.Crossref 4. Albano JDM, Edkins RP, Maritz G, et al: A sensitive precise radioimmunoassay of serum insulin relying on charcoal separation of bound and free hormone moieties . Acta Endocrinol 70:487-509, 1972. 5. Alford F, Bloom SR, Nabarro JDN: Glucagon levels in normal and diabetic subjects: Use of a specific immuno-absorbent for glucagon radioimmunoassay . Diabetologia 13:1-6, 1977.Crossref 6. Jorgensen KH, Larsen UD: Purification of 125I-glucagon by anion exchange chromatography . Horm Metab Res 4:4223-4224, 1972.Crossref
Archives of Surgery – American Medical Association
Published: Jan 1, 1979
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