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Inhibition of a-Chymotrypsin by Aqueous Humor

Inhibition of a-Chymotrypsin by Aqueous Humor Abstract The inhibitory effect of aqueous humor on α-chymotrypsin has recently been demonstrated by Bedrossian and Weimar.1 In the discussion of this paper, however, Dr. B. Schwartz raised the objection that the colorimetric technique, which the authors used, only demonstrated inhibition in terms of liberation of tyrosine groups from hemoglobin molecules. It seemed worthwhile, therefore, to test the effect of aqueous humor on α-chymotrypsin by using a different substrate and a different method. Material and Methods On four consecutive operating days the aqueous humor was collected from the anterior chamber on all routine, uncomplicated cataract sections. A conjunctival flap was reflected and dissected into the cornea for about 1 mm. A knife needle was inserted into the anterior chamber and followed by a 27 gauge blunt needle through which aqueous was aspirated. Fluid was collected from a total of 14 eyes and pooled each day. Aqueous was obtained from four References 1. Supplied as "Zolyse" by Alcon Laboratories. 2. Bedrossian, R. H., and Weimar, V.: Inhibitory Effect of Aqueous Humor on α-chymotrypsin , Trans Amer Acad Ophthal 67:822-828, 1963. 3. Ravin, H. A.; Bernstein, P.; and Seligman, A. M.: Colorimetric Micromethod for Estimation of Chymotrypsin Activity , J Biol Chem 208:1-15, 1954. 4. Seligman, A. M., and Nachlas, M. M.: Colorimetric Determination of Lipase and Esterase in Human Serum , J Clin Invest 29:31-36, 1954.Crossref 5. Pirie, A., and Van Heyningen, R.: Biochemistry of Eye, Springfield , Ill: Charles C Thomas, Publishers, 1956, p 264. 6. Krause, A. C.: Biochemistry of Eye , Baltimore: The Johns Hopkins Press, 1934, p 106. 7. Neisnamoff, E.: Ueber die Quantitativen Verhältnisse der Filtration und Secretion des Kammerwassers , Graefe Arch Ophthal 42:1-35, 1896.Crossref 8. Emmert, E.: Vergleichend-anatomische Untersuchunger über Grössenund Gewichtsverhöltnisse des Auganbels unserer Hausthiere und seiner Bestandtheile . Zvergleich Augenheilk 4:40-70, 1886. 9. Moses, R. A.: " Intra-ocular Circulation and Pressure ", in Modern Ophthalmology , A. Sorsby, ed., Washington: Butterworth & Co., Publishers, Ltd., 1963, vol 1, p 294. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Inhibition of a-Chymotrypsin by Aqueous Humor

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Publisher
American Medical Association
Copyright
Copyright © 1965 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1965.00970030401019
Publisher site
See Article on Publisher Site

Abstract

Abstract The inhibitory effect of aqueous humor on α-chymotrypsin has recently been demonstrated by Bedrossian and Weimar.1 In the discussion of this paper, however, Dr. B. Schwartz raised the objection that the colorimetric technique, which the authors used, only demonstrated inhibition in terms of liberation of tyrosine groups from hemoglobin molecules. It seemed worthwhile, therefore, to test the effect of aqueous humor on α-chymotrypsin by using a different substrate and a different method. Material and Methods On four consecutive operating days the aqueous humor was collected from the anterior chamber on all routine, uncomplicated cataract sections. A conjunctival flap was reflected and dissected into the cornea for about 1 mm. A knife needle was inserted into the anterior chamber and followed by a 27 gauge blunt needle through which aqueous was aspirated. Fluid was collected from a total of 14 eyes and pooled each day. Aqueous was obtained from four References 1. Supplied as "Zolyse" by Alcon Laboratories. 2. Bedrossian, R. H., and Weimar, V.: Inhibitory Effect of Aqueous Humor on α-chymotrypsin , Trans Amer Acad Ophthal 67:822-828, 1963. 3. Ravin, H. A.; Bernstein, P.; and Seligman, A. M.: Colorimetric Micromethod for Estimation of Chymotrypsin Activity , J Biol Chem 208:1-15, 1954. 4. Seligman, A. M., and Nachlas, M. M.: Colorimetric Determination of Lipase and Esterase in Human Serum , J Clin Invest 29:31-36, 1954.Crossref 5. Pirie, A., and Van Heyningen, R.: Biochemistry of Eye, Springfield , Ill: Charles C Thomas, Publishers, 1956, p 264. 6. Krause, A. C.: Biochemistry of Eye , Baltimore: The Johns Hopkins Press, 1934, p 106. 7. Neisnamoff, E.: Ueber die Quantitativen Verhältnisse der Filtration und Secretion des Kammerwassers , Graefe Arch Ophthal 42:1-35, 1896.Crossref 8. Emmert, E.: Vergleichend-anatomische Untersuchunger über Grössenund Gewichtsverhöltnisse des Auganbels unserer Hausthiere und seiner Bestandtheile . Zvergleich Augenheilk 4:40-70, 1886. 9. Moses, R. A.: " Intra-ocular Circulation and Pressure ", in Modern Ophthalmology , A. Sorsby, ed., Washington: Butterworth & Co., Publishers, Ltd., 1963, vol 1, p 294.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Mar 1, 1965

References

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