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Influence of Specific Antibody on Herpes Simplex Infections in Tissue Culture

Influence of Specific Antibody on Herpes Simplex Infections in Tissue Culture Abstract Persons who suffer from recurrent herpes simplex infections have neutralizing or complement-fixing antibodies to herpes simplex virus in their sera, usually in high titer.4,6-9,11-16,18-20 The antibody level in the sera of these patients, according to available evidence,6,9,11-16,19 does not fluctuate in response to the recurrent infection. (The anibody level is as high before the recurrence as it is during or after.) These observations suggest that a high level of specific antibody in the serum is incapable of eradicating the herpes virus even though it may limit its spread. The influence of specific antibody upon the course of experimental herpes infections in tissue cultures has been studied by two groups of investigators. Andrewes3 showed that herpes simplex infections could develop in tissue cultures in the presence of specific antibody providing the virus was added one-half hour before the addition of the antibody. Black and References 1. Inactivation of the serum was carried out by incubation in a water bath at 56 C for one-half hour. Fresh sera which were not treated in this manner inactivated small amounts of herpes simplex virus, although they contained no demonstrable specific antibody. 2. With ST virus, plaque counts were done at 48 hours. Plaque counts were done at 72 hours with CR and HF viruses because plaques were formed more slowly with these strains of virus and counts were sliqhtly more accurate at 72 hours than at 48 hours. 3. Ackermann, W. W.: Mechanisms of Persistent and Masked Infections in Tissue Culture , Ann. New York Acad. Sc. 67:392 ( (April 19) ) 1957.Crossref 4. Ackermann, W. W., and Kurtz, H.: Observations Concerning a Persisting Infection of HeLa Cells with Poliomyelitis Virus , J. Exper. Med. 102:555 ( (Nov.) ) 1955.Crossref 5. Andrewes, C. H.: Tissue-Culture in the Study of Immunity to Herpes , J. Path. & Bact. 33:301, 1930. 6. Andrewes, C. H., and Carmichael, E. H.: A Note on the Presence of Antibodies to Herpes Virus in Post-Encephalitic and Other Human Sera , Lancet 1:857 ( (April 19) ) 1930. 7. Black, F. L., and Melnick, J. L.: MicroEpidemiology of Poliomyelitis and Herpes-B Infections , J. Immunol. 74:236 ( (March) ) 1955. 8. Blank, H., and Rake, G.: Viral and Rickettsial Disease of the Skin, Eye and Mucous Membranes of Man , Boston, Little, Brown & Company, 1955, p. 63. 9. Burnet, F. M., and Lush, D.: Herpes Simplex; Studies on Antibody Content of Human Sera , Lancet 1:629 ( (March 18) ) 1939. 10. Burnet, F. M., and Williams, S. W.: Herpes Simplex: A New Point of View , M. J. Australia 1:637 ( (April 29) ) 1939. 11. Dudgeon, J. A.: A Complement Fixation Test for Herpes Simplex Infections , J. Clin. Path. 3:239 ( (Aug.) ) 1950. 12. Eagle, H.: The Specific Amino Acid Requirements of a Human Carcinoma Cell (Strain HeLa) in Tissue Culture , J. Exper. Med. 102:37 ( (July) ) 1955. 13. Frank, S. B.: Formalized Herpes Virus Therapy and the Neutralizing Substance in Herpes Simplex , J. Invest. Dermat. 1:267 ( (Aug.) ) 1938. 14. Gajdusek, D. C.; Robbins, M. L., and Robbins, F. C.: Diagnosis of Herpes Simplex Infections by the Complement Fixation Test , J. A. M. A. 149:235 ( (May 17) ) 1952. 15. Garabedian, G. A., and Syverton, J. T.: Studies on Herpes Simplex Virus: 1. An Antigenic Analysis of 4 Strains of Virus Isolated from a Human Subject , J. Infect. Dis. 96:1 ( (Jan.) -Feb.) 1955. 16. Holzel, A.; Feldman, G. V.; Tobin, J. O., and Harper, J.: Herpes Simplex: A study of Complement Fixing Antibodies at Different Ages , Acta pædiat. 42:206 ( (May) ) 1953. 17. Jawetz, E.; Allende, M. F., and Coleman, V. R.: Studies on Herpes Simplex Virus: IV. The Level of Neutralizing Antibodies in Human Sera , J. Immunol. 68:655 ( (June) ) 1952. 18. Jawetz, E., Allende, M. F., and Coleman, V. R.: Studies on Herpes Simplex Virus: VI. Observations on Patients with Recurrent Herpetic Lesions Injected with Herpes Viruses or their Antigens , Am. J. M. Sc. 229:477 ( (May) ) 1955. 19. Lazar, M. P.: Vaccination for Recurrent Herpes Simplex Infection , A. M. A. Arch. Dermat. 73:70 ( (Jan.) ) 1956. 20. Nagler, F. P. O.: A Herpes Skin Test Reagent from Amniotic Fluid , Australian J. Exper. Biol. & M. Sc. 24:103 ( (June) ) 1946. 21. Scott, T. F. M.; Coriell, L.; Blank, H., and Burgoon, C. F.: Some Comments on Herpetic Infection in Children with Special Emphasis on Unusual Clinical Manifestations , J. Pediat. 41:835 ( (Dec.) ) 1952. 22. Stark, M. M.; Kibrick, S., and Weisberger, D.: Studies on Recurrent Aphthae: Evidence that Herpes Simplex is Not the Etiological Agent, with Further Observations on the Immune Responses in Herpetic Infections , J. Lab. & Clin. Med. 44:261 ( (Aug.) ) 1954. 23. Syverton, J. T.; Scherer, W. F., and Elwood, P. M.: Studies on the Propagation in Vitro of Poliomyelitis Viruses: V. The Application of Strain HeLa Human Epithelial Cells for Isolation and Typing , J. Lab. & Clin. Med. 43:286 ( (Feb.) ) 1954. 24. Wheeler, C. E.: The Effect of Temperature upon the Production of Herpes Simplex Virus in Tissue Culture , J. Immunol. , to be published. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png A.M.A. Archives of Dermatology American Medical Association

Influence of Specific Antibody on Herpes Simplex Infections in Tissue Culture

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Publisher
American Medical Association
Copyright
Copyright © 1959 American Medical Association. All Rights Reserved.
ISSN
0096-5359
DOI
10.1001/archderm.1959.01560130088010
Publisher site
See Article on Publisher Site

Abstract

Abstract Persons who suffer from recurrent herpes simplex infections have neutralizing or complement-fixing antibodies to herpes simplex virus in their sera, usually in high titer.4,6-9,11-16,18-20 The antibody level in the sera of these patients, according to available evidence,6,9,11-16,19 does not fluctuate in response to the recurrent infection. (The anibody level is as high before the recurrence as it is during or after.) These observations suggest that a high level of specific antibody in the serum is incapable of eradicating the herpes virus even though it may limit its spread. The influence of specific antibody upon the course of experimental herpes infections in tissue cultures has been studied by two groups of investigators. Andrewes3 showed that herpes simplex infections could develop in tissue cultures in the presence of specific antibody providing the virus was added one-half hour before the addition of the antibody. Black and References 1. Inactivation of the serum was carried out by incubation in a water bath at 56 C for one-half hour. Fresh sera which were not treated in this manner inactivated small amounts of herpes simplex virus, although they contained no demonstrable specific antibody. 2. With ST virus, plaque counts were done at 48 hours. Plaque counts were done at 72 hours with CR and HF viruses because plaques were formed more slowly with these strains of virus and counts were sliqhtly more accurate at 72 hours than at 48 hours. 3. Ackermann, W. W.: Mechanisms of Persistent and Masked Infections in Tissue Culture , Ann. New York Acad. Sc. 67:392 ( (April 19) ) 1957.Crossref 4. Ackermann, W. W., and Kurtz, H.: Observations Concerning a Persisting Infection of HeLa Cells with Poliomyelitis Virus , J. Exper. Med. 102:555 ( (Nov.) ) 1955.Crossref 5. Andrewes, C. H.: Tissue-Culture in the Study of Immunity to Herpes , J. Path. & Bact. 33:301, 1930. 6. Andrewes, C. H., and Carmichael, E. H.: A Note on the Presence of Antibodies to Herpes Virus in Post-Encephalitic and Other Human Sera , Lancet 1:857 ( (April 19) ) 1930. 7. Black, F. L., and Melnick, J. L.: MicroEpidemiology of Poliomyelitis and Herpes-B Infections , J. Immunol. 74:236 ( (March) ) 1955. 8. Blank, H., and Rake, G.: Viral and Rickettsial Disease of the Skin, Eye and Mucous Membranes of Man , Boston, Little, Brown & Company, 1955, p. 63. 9. Burnet, F. M., and Lush, D.: Herpes Simplex; Studies on Antibody Content of Human Sera , Lancet 1:629 ( (March 18) ) 1939. 10. Burnet, F. M., and Williams, S. W.: Herpes Simplex: A New Point of View , M. J. Australia 1:637 ( (April 29) ) 1939. 11. Dudgeon, J. A.: A Complement Fixation Test for Herpes Simplex Infections , J. Clin. Path. 3:239 ( (Aug.) ) 1950. 12. Eagle, H.: The Specific Amino Acid Requirements of a Human Carcinoma Cell (Strain HeLa) in Tissue Culture , J. Exper. Med. 102:37 ( (July) ) 1955. 13. Frank, S. B.: Formalized Herpes Virus Therapy and the Neutralizing Substance in Herpes Simplex , J. Invest. Dermat. 1:267 ( (Aug.) ) 1938. 14. Gajdusek, D. C.; Robbins, M. L., and Robbins, F. C.: Diagnosis of Herpes Simplex Infections by the Complement Fixation Test , J. A. M. A. 149:235 ( (May 17) ) 1952. 15. Garabedian, G. A., and Syverton, J. T.: Studies on Herpes Simplex Virus: 1. An Antigenic Analysis of 4 Strains of Virus Isolated from a Human Subject , J. Infect. Dis. 96:1 ( (Jan.) -Feb.) 1955. 16. Holzel, A.; Feldman, G. V.; Tobin, J. O., and Harper, J.: Herpes Simplex: A study of Complement Fixing Antibodies at Different Ages , Acta pædiat. 42:206 ( (May) ) 1953. 17. Jawetz, E.; Allende, M. F., and Coleman, V. R.: Studies on Herpes Simplex Virus: IV. The Level of Neutralizing Antibodies in Human Sera , J. Immunol. 68:655 ( (June) ) 1952. 18. Jawetz, E., Allende, M. F., and Coleman, V. R.: Studies on Herpes Simplex Virus: VI. Observations on Patients with Recurrent Herpetic Lesions Injected with Herpes Viruses or their Antigens , Am. J. M. Sc. 229:477 ( (May) ) 1955. 19. Lazar, M. P.: Vaccination for Recurrent Herpes Simplex Infection , A. M. A. Arch. Dermat. 73:70 ( (Jan.) ) 1956. 20. Nagler, F. P. O.: A Herpes Skin Test Reagent from Amniotic Fluid , Australian J. Exper. Biol. & M. Sc. 24:103 ( (June) ) 1946. 21. Scott, T. F. M.; Coriell, L.; Blank, H., and Burgoon, C. F.: Some Comments on Herpetic Infection in Children with Special Emphasis on Unusual Clinical Manifestations , J. Pediat. 41:835 ( (Dec.) ) 1952. 22. Stark, M. M.; Kibrick, S., and Weisberger, D.: Studies on Recurrent Aphthae: Evidence that Herpes Simplex is Not the Etiological Agent, with Further Observations on the Immune Responses in Herpetic Infections , J. Lab. & Clin. Med. 44:261 ( (Aug.) ) 1954. 23. Syverton, J. T.; Scherer, W. F., and Elwood, P. M.: Studies on the Propagation in Vitro of Poliomyelitis Viruses: V. The Application of Strain HeLa Human Epithelial Cells for Isolation and Typing , J. Lab. & Clin. Med. 43:286 ( (Feb.) ) 1954. 24. Wheeler, C. E.: The Effect of Temperature upon the Production of Herpes Simplex Virus in Tissue Culture , J. Immunol. , to be published.

Journal

A.M.A. Archives of DermatologyAmerican Medical Association

Published: Jan 1, 1959

References