Abstract Research continues on several "biophysical" parameters which can influence the survival of the human red cell under conditions of blood-bank storage, for example, the type of container, above-freezing temperature, buffer, pH, anticoagulants, dehydrants, etc. While supplying worthwhile information for improvement in the art of blood-banking, these approaches do not at the present appear destined to yield any major extension of red cell life. Another biophysical approach, the complete suspension of degradative changes by deep freeze, has led to recent notable advances. There is now no question that red cells can be stored at —80 C or below in the presence of glycerol for months or even years, and be thawed under appropriate conditions to give an essentially normal post-transfusion lifespan. Rapid progress is being made in the development of techniques for processing the frozen red cells, particularly through the combined efforts of the Protein Foundation in Boston and the Chelsea References 1. Mollison, P. L.: Blood Transfusion in Clinical Medicine , Ed. 2, Springfield, Ill., Charles C Thomas, Publisher, 1956, Chap. I. 2. Bartlett, G. R.: Human Red Cell Glycolytic Intermediates , J. Biol. Chem. 234:449, 1959. 3. Gabrio, B. W.; Finch, C. A., and Huennekens, F. M.: Erythrocyte Preservation: A Topic in Molecular Biochemistry , Blood 11:103, 1956. 4. Gabrio, B. W.; Donohue, D. M., and Finch, C. A.: Erythrocyte Preservation: V. Relationship Between Chemical Changes and Viability of Stored Blood Treated with Adenosine , J. Clin. Invest. 34:1509, 1955.Crossref 5. Donohue, D. M.; Finch, C. A., and Gabrio, B. W.: Erythrocyte Preservation: VI. The Storage of Blood with Purine Nucleosides , J. Clin. Invest. 35:562, 1956.Crossref 6. Gabrio, B. W.; Donohue, D. M.; Huennekens, F. M., and Finch, C. A.: Erythrocyte Preservation: VII. Acid-Citrate-Dextrose-Inosine (ACDI) as a Preservative for Blood During Storage at 4C , J. Clin. Invest. 35:657, 1956.Crossref 7. Gibson, J. G., II, and Scheitlin, W. A.: A Method Employing Radioactive Chromium for Assaying the Viability of Human Erythrocytes Returned to the Circulation After Refrigerated Storage , J. Lab. & Clin. Med. 46:679, 1955. 8. Lange, R. D.; Crosby, W. H.; Donohue, D. M.; Finch, C. A.; Gibson, J. G., II; McManus, T. J., and Strumia, M. M.: Effect of Inosine on Red Cell Preservation , J. Clin. Invest. 37:1485, 1958. 9. Shafer, A. W., and Bartlett, G. R.: Effect of Inosine on the Post-Transfusion Survival of Stored Rabbit Erythrocytes , J. Clin. Invest. 39:69, 1960. 10. Mollison, P. L., and Robinson, M. A.: Observations of the Effects of Purine Nucleosides on Red-Cell Preservation , Brit. J. Haemat. 5:331, 1959. 11. Nakao, M.; Nakao, T.; Tatibana, M., and Yoshikawa, H.: Phosphorus Metabolism in Human Erythrocyte: III. Regeneration of Adenosine Triphosphate in Long-Stored Erythrocyte by Incubation with Inosine and Adenine , J. Biochem. 47:661, 1960. 12. Shafer, A. W., and Bartlett, G. R.: Unpublished observations. 13. Bucolo, G., and Bartlett, G. R.: Unpublished observations. 14. Horecker, B. L., and Hiatt, H. H.: Pathways of Carbohydrate Metabolism in Normal and Neoplastic Cells , New England J. Med. 258:177, 1958. 15. Huennekens, F. M.; Nurk, E., and Gabrio, B. W.: Erythrocyte Metabolism: I. Purine Nucleoside Phosphorylase , J. Biol. Chem. 221:971, 1956. 16. Tsuboi, K. K., and Hudson, P. B.: Enzymes of the Human Erythrocyte , J. Biol. Chem. 224:879, 889, 1957.
Archives of Internal Medicine – American Medical Association
Published: Dec 1, 1960