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Induction of Hepatocyte Lipopolysaccharide Binding Protein in Models of Sepsis and the Acute-Phase Response

Induction of Hepatocyte Lipopolysaccharide Binding Protein in Models of Sepsis and the... Abstract • Lipopolysaccharide binding protein (LBP) is a serum glycoprotein that complexes with lipopolysaccharide (LPS) to facilitate macrophage response to endotoxin. To determine the conditions that stimulate LBP production in vivo, we measured the induction of LBP in models of inflammation produced by LPS, Corynebacteriumparvum, and turpentine injection. Plasma aspartate aminotransferase and alanine aminotransferase concentrations and hepatocyte fibrinogen synthesis were elevated in all models. Northern blot analysis revealed 17-, 14-, and 20-fold upregulation of hepatocyte LBP mRNA following treatment with LPS, C parvum, and turpentine, respectively. Peritoneal macrophage interleukin 6 and tumor necrosis factor production following endotoxin stimulation was augmented by cultured hepatocyte supernatants, suggesting increased LBP synthesis in these groups. The results show that LBP mRNA is induced during hepatic inflammation and suggest that LBP is an acute-phase protein important in regulating the in vivo response to endotoxin. (Arch Surg. 1993;128:22-28) References 1. Parrillo JE, Parker M, Natanson C, et al. Septic shock in humans: advances in the understanding of pathogenesis, cardiovascular dysfunction, and therapy . Ann Intern Med . 1990;113:227-242.Crossref 2. Barton R, Cerra FB. The hypermetabolism multiple organ failure syndrome . Chest . 1989;5:1153-1160.Crossref 3. Dinarello C. Interleukin-1 and its biologically related cytokines . Adv Immunol . 1989;44:153-205. 4. Okusawa S, Gelfand JA, Ikejima T, Connolly RJ, Dinarello DA. Interleukin-1 induces a shock-like state in rabbits: synergism with tumor necrosis factor and the effect of cyclooxygenase inhibition . J Clin Invest . 1988; 81:1162-1172.Crossref 5. Tracey KJ, Beutler B, Lowry SF, et al. Shock and tissue injury induced by recombinant human cachectin . Science . 1986;234:470-474.Crossref 6. Beutler B, Cerami A. Cachectin: more than a tumor necrosis factor . N Engl J Med . 1987;316:379-385.Crossref 7. Sherry B, Cerami A. Cachectin/tumor necrosis factor exerts endocrine, paracrine, and autocrine control of inflammatory responses . J Cell Biol . 1988;107:1269-1277.Crossref 8. Tobias PS, Soldau K, Ulevitch RJ. Isolation of a lipopolysaccharide binding acute phase reactant from rabbit serum . J Exp Med . 1986;164:777-793.Crossref 9. Ramadori G, Meyer zum Buschenfelde KH, Tobias PS, Mathison JC, Ulevitch RJ. Biosynthesis of lipopolysaccharide-binding protein in rabbit hepatocytes . Pathobiology . 1990;58:89-94.Crossref 10. Schumann RR, Leong SR, Flaggs GW, et al. Structure and function of lipopolysaccharide binding protein . Science . 1990;249:1429-1431.Crossref 11. Wright SD, Ramos RA, Tobias PS, Ulevitch RJ, Mathison JC. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein . Science . 1990;249:1431-1433.Crossref 12. West MA, Keller GA, Cerra FB, Simmons RL. Killed Escherichia coli stimulates macrophage mediated alterations in hepatocellular function during in vitro coculture: a mechanism of altered liver function in sepsis . Infect Immun . 1985;49:563-570. 13. Kispert PH, Curran R, Billiar TR, Simmons RL. Kupffer cells can provide the signal for hepatocyte acute phase protein synthesis . Surg Forum . 1990;41:80-82. 14. Billiar TR, Curran RD, Williams DL, Kispert P. Liver nonparenchymal cells are stimulated to provide IL-6 for induction of the hepatic acute-phase response in endotoxemia but not in remote localized inflammation . Arch Surg . 1992;127:31-37.Crossref 15. Billiar TR, Lysz TW, Curran RD, Bentz BG, Machiedo GW, Simmons RL. Hepatocyte regulation of Kupffer cell prostaglandin E2 production in vitro . J Leukoc Biol . 1990;47:304-311. 16. Lysz TW, Billiar TR, Curran RD, Simmons RL, Machiedo GW. Kupffer cell-hepatocyte interactions and the changes in 14G-arachidonate incorporation in response to endotoxin in vitro . Prostaglandins 1990;39:497-514.Crossref 17. Curran RD, Billiar TR, Kispert PH, Bentz BG, May MT, Simmons RL. Hepatocytes enhance Kupffer cell-mediated tumor cell cytostasis in vitro . Surgery . 1989;106:126-132. 18. Billiar TR, Curran RD, Lysz TW, Ferrari FK, Machiedo GW, Simmons RL. Hepatocytes promote Kupffer cell TNF and IL-1 production in response to LPS . FASEB J . 1989;3:A637. 19. Green S, Dobrjansky. Corynebacterium parvum: effects on the biochemistry of mouse serum and liver . J Natl Cancer Inst . 1979;63:497-502. 20. Hart D. Increased sensitivity of Corynebacterium parvum-treated mice to toxic effects of indomethacin and lipopolysaccharide . Infect Immun . 1985;47:408-414. 21. Northemann W, Andus T, Gross V, Heinrich PC. Cell-free synthesis of rat α2-macroglobulin and induction of its mRNA during experimental inflammation . Eur J Biochem . 1983;137:257-262.Crossref 22. Fuller GM, Otto JM, Woloski M, McGary CT, Adams MA. The effects of hepatocyte-stimulating factor on fibrinogen biosynthesis in hepatocyte monolayers . J Cell Biol . 1985;101:1481-1486.Crossref 23. Gauldie J, Richards C, Harnish D, Lansdorp P, Baumann H. Interferon B2/B-cell stimulatory factor type 2 shares identity with monocyte derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells . Proc Natl Acad Sci USA . 1987;84:7251-7255.Crossref 24. Su GL, Wang SC, Geller DA, et al. Molecular cloning of rat lipopolysaccharide binding protein . J Cell Biochem . 1992;16c:162. 25. LaDuca FM, Dang CV, Bell WR. Application of a nitrocellulose immunoassay for quantitation of proteins secreted in culture media . Ann Biochem . 1986;158:262-267.Crossref 26. Seglen PO. Preparation of isolated rat liver cells . Methods Cell Biol . 1976;13:29-83. 27. Billiar T, Curran R, Stadler J, et al. Induction of nitric oxide production by liver cells in vivo . Surg Forum . 1990;41:64-66. 28. Aarden LA, DeGroat OL, Schapp OL, Landsdorf PM. Production of hybridoma growth factor by human monocytes . Eur J Immunol . 1987;17: 1411-1416.Crossref 29. Aggarwal B, Kohr W, Hass P, et al. Human tumor necrosis factor: production purification and characterization . J Biol Chem . 1985;260:2354. 30. Genovese C, Milcarek C. Increased half-life of μ immunoglobulin mRNA during mouse B cell development increases its abundancy . Mol Immunol . 1990;27:733-743.Crossref 31. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction . Anal Biochem . 1987;162:156-159.Crossref 32. Maniatis T, Fritsch EF, Sambrook J. Molecular Cloning: A Laboratory Manual . Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1982. 33. Amasino RM. Acceleration of nucleic acid hybridization rate by polyethylene glycol . Anal Biochem . 1986;152:304-307.Crossref 34. Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity . Anal Biochem . 1983;132:6-13.Crossref 35. Berendt MJ, Newborg MF, North RJ. Increased toxicity of endotoxin for tumor-bearing mice and mice responding to bacterial pathogens: macrophage activation as a common denominator . Infect Immun . 1980;28: 645-647. 36. Yoshikai Y, Miake S, Sano M, Nomoto K. Increased susceptibility to Escherichia coliinfections in mice pretreated with Corynebacterium parvum . Microbiol Immunol . 1983;27:273-282.Crossref 37. Raetz CRH, Ulevitch RJ, Wright SD, Sibley CH, Ding A, Nathan CF. Gram-negative endotoxin: an extraordinary lipid with profound effects on eukaryotic signal transduction . FASEB J . 1991;5:2652-2660. 38. Landmann R, Fisscher A, Obrecht JP. Soluble CD14 release in human monocytes and macrophages, regulation by interferon-gamma and interleukin-4 . J Cell Biochem . 1992;16c:164. 39. Haziot A, Rong GW, Tseuberi BZ, Goyert SM. Soluble CD14 inhibits the activation of monocytes induced by LPS . J Cell Biochem . 1992;16c:163. 40. Wright S, Tobias PS, Ulevitch RJ, Ramos RA. Lipopolysaccharide binding protein opsonizes LPS-bearing particles for recognition by a novel receptor on macrophages . J Exp Med . 1989;170:1231-1241.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

Induction of Hepatocyte Lipopolysaccharide Binding Protein in Models of Sepsis and the Acute-Phase Response

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Publisher
American Medical Association
Copyright
Copyright © 1993 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1993.01420130026005
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Abstract

Abstract • Lipopolysaccharide binding protein (LBP) is a serum glycoprotein that complexes with lipopolysaccharide (LPS) to facilitate macrophage response to endotoxin. To determine the conditions that stimulate LBP production in vivo, we measured the induction of LBP in models of inflammation produced by LPS, Corynebacteriumparvum, and turpentine injection. Plasma aspartate aminotransferase and alanine aminotransferase concentrations and hepatocyte fibrinogen synthesis were elevated in all models. Northern blot analysis revealed 17-, 14-, and 20-fold upregulation of hepatocyte LBP mRNA following treatment with LPS, C parvum, and turpentine, respectively. Peritoneal macrophage interleukin 6 and tumor necrosis factor production following endotoxin stimulation was augmented by cultured hepatocyte supernatants, suggesting increased LBP synthesis in these groups. The results show that LBP mRNA is induced during hepatic inflammation and suggest that LBP is an acute-phase protein important in regulating the in vivo response to endotoxin. (Arch Surg. 1993;128:22-28) References 1. Parrillo JE, Parker M, Natanson C, et al. Septic shock in humans: advances in the understanding of pathogenesis, cardiovascular dysfunction, and therapy . Ann Intern Med . 1990;113:227-242.Crossref 2. Barton R, Cerra FB. The hypermetabolism multiple organ failure syndrome . Chest . 1989;5:1153-1160.Crossref 3. Dinarello C. Interleukin-1 and its biologically related cytokines . Adv Immunol . 1989;44:153-205. 4. Okusawa S, Gelfand JA, Ikejima T, Connolly RJ, Dinarello DA. Interleukin-1 induces a shock-like state in rabbits: synergism with tumor necrosis factor and the effect of cyclooxygenase inhibition . J Clin Invest . 1988; 81:1162-1172.Crossref 5. Tracey KJ, Beutler B, Lowry SF, et al. Shock and tissue injury induced by recombinant human cachectin . Science . 1986;234:470-474.Crossref 6. Beutler B, Cerami A. Cachectin: more than a tumor necrosis factor . N Engl J Med . 1987;316:379-385.Crossref 7. Sherry B, Cerami A. Cachectin/tumor necrosis factor exerts endocrine, paracrine, and autocrine control of inflammatory responses . J Cell Biol . 1988;107:1269-1277.Crossref 8. Tobias PS, Soldau K, Ulevitch RJ. Isolation of a lipopolysaccharide binding acute phase reactant from rabbit serum . J Exp Med . 1986;164:777-793.Crossref 9. Ramadori G, Meyer zum Buschenfelde KH, Tobias PS, Mathison JC, Ulevitch RJ. Biosynthesis of lipopolysaccharide-binding protein in rabbit hepatocytes . Pathobiology . 1990;58:89-94.Crossref 10. Schumann RR, Leong SR, Flaggs GW, et al. Structure and function of lipopolysaccharide binding protein . Science . 1990;249:1429-1431.Crossref 11. Wright SD, Ramos RA, Tobias PS, Ulevitch RJ, Mathison JC. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein . Science . 1990;249:1431-1433.Crossref 12. West MA, Keller GA, Cerra FB, Simmons RL. Killed Escherichia coli stimulates macrophage mediated alterations in hepatocellular function during in vitro coculture: a mechanism of altered liver function in sepsis . Infect Immun . 1985;49:563-570. 13. Kispert PH, Curran R, Billiar TR, Simmons RL. Kupffer cells can provide the signal for hepatocyte acute phase protein synthesis . Surg Forum . 1990;41:80-82. 14. Billiar TR, Curran RD, Williams DL, Kispert P. Liver nonparenchymal cells are stimulated to provide IL-6 for induction of the hepatic acute-phase response in endotoxemia but not in remote localized inflammation . Arch Surg . 1992;127:31-37.Crossref 15. Billiar TR, Lysz TW, Curran RD, Bentz BG, Machiedo GW, Simmons RL. Hepatocyte regulation of Kupffer cell prostaglandin E2 production in vitro . J Leukoc Biol . 1990;47:304-311. 16. Lysz TW, Billiar TR, Curran RD, Simmons RL, Machiedo GW. Kupffer cell-hepatocyte interactions and the changes in 14G-arachidonate incorporation in response to endotoxin in vitro . Prostaglandins 1990;39:497-514.Crossref 17. Curran RD, Billiar TR, Kispert PH, Bentz BG, May MT, Simmons RL. Hepatocytes enhance Kupffer cell-mediated tumor cell cytostasis in vitro . Surgery . 1989;106:126-132. 18. Billiar TR, Curran RD, Lysz TW, Ferrari FK, Machiedo GW, Simmons RL. Hepatocytes promote Kupffer cell TNF and IL-1 production in response to LPS . FASEB J . 1989;3:A637. 19. Green S, Dobrjansky. Corynebacterium parvum: effects on the biochemistry of mouse serum and liver . J Natl Cancer Inst . 1979;63:497-502. 20. Hart D. Increased sensitivity of Corynebacterium parvum-treated mice to toxic effects of indomethacin and lipopolysaccharide . Infect Immun . 1985;47:408-414. 21. Northemann W, Andus T, Gross V, Heinrich PC. Cell-free synthesis of rat α2-macroglobulin and induction of its mRNA during experimental inflammation . Eur J Biochem . 1983;137:257-262.Crossref 22. Fuller GM, Otto JM, Woloski M, McGary CT, Adams MA. The effects of hepatocyte-stimulating factor on fibrinogen biosynthesis in hepatocyte monolayers . J Cell Biol . 1985;101:1481-1486.Crossref 23. Gauldie J, Richards C, Harnish D, Lansdorp P, Baumann H. Interferon B2/B-cell stimulatory factor type 2 shares identity with monocyte derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells . Proc Natl Acad Sci USA . 1987;84:7251-7255.Crossref 24. Su GL, Wang SC, Geller DA, et al. Molecular cloning of rat lipopolysaccharide binding protein . J Cell Biochem . 1992;16c:162. 25. LaDuca FM, Dang CV, Bell WR. Application of a nitrocellulose immunoassay for quantitation of proteins secreted in culture media . Ann Biochem . 1986;158:262-267.Crossref 26. Seglen PO. Preparation of isolated rat liver cells . Methods Cell Biol . 1976;13:29-83. 27. Billiar T, Curran R, Stadler J, et al. Induction of nitric oxide production by liver cells in vivo . Surg Forum . 1990;41:64-66. 28. Aarden LA, DeGroat OL, Schapp OL, Landsdorf PM. Production of hybridoma growth factor by human monocytes . Eur J Immunol . 1987;17: 1411-1416.Crossref 29. Aggarwal B, Kohr W, Hass P, et al. Human tumor necrosis factor: production purification and characterization . J Biol Chem . 1985;260:2354. 30. Genovese C, Milcarek C. Increased half-life of μ immunoglobulin mRNA during mouse B cell development increases its abundancy . Mol Immunol . 1990;27:733-743.Crossref 31. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction . Anal Biochem . 1987;162:156-159.Crossref 32. Maniatis T, Fritsch EF, Sambrook J. Molecular Cloning: A Laboratory Manual . Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1982. 33. Amasino RM. Acceleration of nucleic acid hybridization rate by polyethylene glycol . Anal Biochem . 1986;152:304-307.Crossref 34. Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity . Anal Biochem . 1983;132:6-13.Crossref 35. Berendt MJ, Newborg MF, North RJ. Increased toxicity of endotoxin for tumor-bearing mice and mice responding to bacterial pathogens: macrophage activation as a common denominator . Infect Immun . 1980;28: 645-647. 36. Yoshikai Y, Miake S, Sano M, Nomoto K. Increased susceptibility to Escherichia coliinfections in mice pretreated with Corynebacterium parvum . Microbiol Immunol . 1983;27:273-282.Crossref 37. Raetz CRH, Ulevitch RJ, Wright SD, Sibley CH, Ding A, Nathan CF. Gram-negative endotoxin: an extraordinary lipid with profound effects on eukaryotic signal transduction . FASEB J . 1991;5:2652-2660. 38. Landmann R, Fisscher A, Obrecht JP. Soluble CD14 release in human monocytes and macrophages, regulation by interferon-gamma and interleukin-4 . J Cell Biochem . 1992;16c:164. 39. Haziot A, Rong GW, Tseuberi BZ, Goyert SM. Soluble CD14 inhibits the activation of monocytes induced by LPS . J Cell Biochem . 1992;16c:163. 40. Wright S, Tobias PS, Ulevitch RJ, Ramos RA. Lipopolysaccharide binding protein opsonizes LPS-bearing particles for recognition by a novel receptor on macrophages . J Exp Med . 1989;170:1231-1241.Crossref

Journal

Archives of SurgeryAmerican Medical Association

Published: Jan 1, 1993

References