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Increased Susceptibility to Infection due to Infusion of Exogenous Chemotaxin

Increased Susceptibility to Infection due to Infusion of Exogenous Chemotaxin Abstract • Previous studies indicate that endogenous chemotaxins, such as the chemotactic factor C5a, may modulate the function of neutrophils (PMNs) and account for increased susceptibility to infection after injury. These effects were investigated by continuously infusing rats with saline or the chemotaxin formyl-methionyl-leucyl-phenylalanine (FMLP). Rats that sustained a full-thickness burn covering 30% of total body surface area and whose wounds were inoculated with Pseudomonas aeruginosa had a significantly shorter survival when FMLP was infused (6.5±0.91 days) than did saline-infused rats (9.9±0.83 days). Rats infused with FMLP had significantly more leukocytes in their burn wounds, significantly fewer PMNs in the circulating pool, and the same number of PMNs at the site of FMLP infusion compared with the saline-treated group. These findings support the hypothesis that chemotaxins generated by tissue injury or sepsis contribute to increased susceptibility to infection. (Arch Surg 1987;122:111-116) References 1. Yurt RW, McManus AT, Mason AD, et al: Increased susceptibility to infection related to extent of burn injury . Arch Surg 1984;119:183-188.Crossref 2. Yurt RW, Pruitt BA: Decreased wound neutrophils and indiscrete margination in the pathogenesis of wound infection . Surgery 1985;95: 191-198. 3. Solomkin JS, Cotta LA, Ogle JD, et al: Complement-induced expression of cryptic receptors on the neutrophil surface: A mechanism for regulation of acute inflammation in trauma . Surgery 1984;96:336-342. 4. Solomkin JS, Nelson RD, Chenoweth DE, et al: Regulation of neutrophil migratory function in burn injury by complement activation products . Ann Surg 1984;200:742-746.Crossref 5. Moore FD, Davis C, Rodrick M, et al: Neutrophil activation in thermal injury as assessed by increased expression of complement receptors . N Engl J Med 1986;314:948-953.Crossref 6. Walker HL, Mason AD Jr: A standard animal burn . J Trauma 1968;8:1049-1051.Crossref 7. Lindberg RB, Moncrief JA, Mason AD Jr: Control of experimental and clinical burn wound sepsis by topical application of Sulfamylon compounds . Ann NY Acad Sci 1968;150:950-960.Crossref 8. McManus AT, McLeod CG Jr, Mason AD Jr: Experimental Proteus mirabilis burn surface infection . Arch Surg 1982;117:187-191.Crossref 9. Harms PG, Ojeda JR: A rapid and simple procedure for chronic cannulation of the rat jugular vein . J Appl Physiol 1974;36:391-394. 10. Gilbertsen RB, Carter GW, Quinn DJ: Effects of F-Met-Leu-Phe and zymosan-activated serum on rat neutrophils in vivo . J Reticuloendothel Soc 1980;27:485-494. 11. Zar JH: Multiple comparisons , in McElroy WD, Swanson CP (eds): Biostatistical Analysis . Englewood Cliffs, NJ, Prentice-Hall Inc, 1974, pp 151-154. 12. Fletcher MP, Seligmann BE, Gallin JI: Correlation of human neutrophil secretion, chemoattractant receptor mobilization, and enhanced functional capacity . J Immunol 1982;128:941-948. 13. Marasco WA, Feltner DE, Ward PE: Formyl peptide chemotaxis receptors on the rat neutrophil: Experimental evidence for negative cooperativity . J Cell Biochem 1985;27:359-375.Crossref 14. Chester JF, Ross JS, Malt RA, et al: Acute colitis produced by chemotactic peptides in rats and mice . Am J Pathol 1985;121:284-290. 15. McCall CE, Bass DA, DeChatelet LR, et al: In vitro responses of human neutrophils to N-formyl-methionyl-leucyl-phenylalanine: Correlation with effects of acute bacterial infection . J Infect Dis 1979;140:277-286.Crossref 16. Nelson RD, McCormack RT, Fiegel VD, et al: Chemotactic deactivation of human neutrophils: Evidence for nonspecific and specific components . Infect Immun 1978;22:441-444. 17. Donabedian H, Gallin JI: Deactivation of human neutrophil chemotaxis by chemoattractants: Effect on receptors for the chemotactic factor f-Met-Leu-Phe . J Immunol 1981;127:839-844. 18. Center DM, Soter NA, Wasserman SI, et al: Inhibition of neutrophil chemotaxis in association with experimental angioedema in patients with cold urticaria: A model of chemotactic deactivation in vivo . Clin Exp Immunol 1979;35:112-118. 19. Solomkin JS, Jenkins MK, Nelson RD, et al: Neutrophil dysfunction in sepsis: II. Evidence for the role of complement activation products in cellular deactivation . Surgery 1981;90:319-327. 20. Van Epps DE, Garcia ML: Enhancement of neutrophil function as a result of prior exposure to chemotactic factor . J Clin Invest 1980;66: 167-175.Crossref 21. Gallin JI, Wright DG, Schiffman E: Role of secretory events in modulating human neutrophil chemotaxis . J Clin Invest 1978;62:1364-1374.Crossref 22. Kay AB: Complement receptor enhancement by chemotactic factors . Mol Immunol 1982;19:1307-1311.Crossref 23. Fearon DT, Collins LA: Increased expression of C3b on polymorpho-nuclear leukocytes induced by chemotactic factors and by purification procedures . J Immunol 1983;130:370-375. 24. Schiffmann E, Corcoran BA, Wahl SM: N-formylmethionyl peptides as chemoattractants for leukocytes . Proc Natl Acad Sci USA 1975;72: 1059-1062.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

Increased Susceptibility to Infection due to Infusion of Exogenous Chemotaxin

Archives of Surgery , Volume 122 (1) – Jan 1, 1987

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Publisher
American Medical Association
Copyright
Copyright © 1987 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1987.01400130117018
Publisher site
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Abstract

Abstract • Previous studies indicate that endogenous chemotaxins, such as the chemotactic factor C5a, may modulate the function of neutrophils (PMNs) and account for increased susceptibility to infection after injury. These effects were investigated by continuously infusing rats with saline or the chemotaxin formyl-methionyl-leucyl-phenylalanine (FMLP). Rats that sustained a full-thickness burn covering 30% of total body surface area and whose wounds were inoculated with Pseudomonas aeruginosa had a significantly shorter survival when FMLP was infused (6.5±0.91 days) than did saline-infused rats (9.9±0.83 days). Rats infused with FMLP had significantly more leukocytes in their burn wounds, significantly fewer PMNs in the circulating pool, and the same number of PMNs at the site of FMLP infusion compared with the saline-treated group. These findings support the hypothesis that chemotaxins generated by tissue injury or sepsis contribute to increased susceptibility to infection. (Arch Surg 1987;122:111-116) References 1. Yurt RW, McManus AT, Mason AD, et al: Increased susceptibility to infection related to extent of burn injury . Arch Surg 1984;119:183-188.Crossref 2. Yurt RW, Pruitt BA: Decreased wound neutrophils and indiscrete margination in the pathogenesis of wound infection . Surgery 1985;95: 191-198. 3. Solomkin JS, Cotta LA, Ogle JD, et al: Complement-induced expression of cryptic receptors on the neutrophil surface: A mechanism for regulation of acute inflammation in trauma . Surgery 1984;96:336-342. 4. Solomkin JS, Nelson RD, Chenoweth DE, et al: Regulation of neutrophil migratory function in burn injury by complement activation products . Ann Surg 1984;200:742-746.Crossref 5. Moore FD, Davis C, Rodrick M, et al: Neutrophil activation in thermal injury as assessed by increased expression of complement receptors . N Engl J Med 1986;314:948-953.Crossref 6. Walker HL, Mason AD Jr: A standard animal burn . J Trauma 1968;8:1049-1051.Crossref 7. Lindberg RB, Moncrief JA, Mason AD Jr: Control of experimental and clinical burn wound sepsis by topical application of Sulfamylon compounds . Ann NY Acad Sci 1968;150:950-960.Crossref 8. McManus AT, McLeod CG Jr, Mason AD Jr: Experimental Proteus mirabilis burn surface infection . Arch Surg 1982;117:187-191.Crossref 9. Harms PG, Ojeda JR: A rapid and simple procedure for chronic cannulation of the rat jugular vein . J Appl Physiol 1974;36:391-394. 10. Gilbertsen RB, Carter GW, Quinn DJ: Effects of F-Met-Leu-Phe and zymosan-activated serum on rat neutrophils in vivo . J Reticuloendothel Soc 1980;27:485-494. 11. Zar JH: Multiple comparisons , in McElroy WD, Swanson CP (eds): Biostatistical Analysis . Englewood Cliffs, NJ, Prentice-Hall Inc, 1974, pp 151-154. 12. Fletcher MP, Seligmann BE, Gallin JI: Correlation of human neutrophil secretion, chemoattractant receptor mobilization, and enhanced functional capacity . J Immunol 1982;128:941-948. 13. Marasco WA, Feltner DE, Ward PE: Formyl peptide chemotaxis receptors on the rat neutrophil: Experimental evidence for negative cooperativity . J Cell Biochem 1985;27:359-375.Crossref 14. Chester JF, Ross JS, Malt RA, et al: Acute colitis produced by chemotactic peptides in rats and mice . Am J Pathol 1985;121:284-290. 15. McCall CE, Bass DA, DeChatelet LR, et al: In vitro responses of human neutrophils to N-formyl-methionyl-leucyl-phenylalanine: Correlation with effects of acute bacterial infection . J Infect Dis 1979;140:277-286.Crossref 16. Nelson RD, McCormack RT, Fiegel VD, et al: Chemotactic deactivation of human neutrophils: Evidence for nonspecific and specific components . Infect Immun 1978;22:441-444. 17. Donabedian H, Gallin JI: Deactivation of human neutrophil chemotaxis by chemoattractants: Effect on receptors for the chemotactic factor f-Met-Leu-Phe . J Immunol 1981;127:839-844. 18. Center DM, Soter NA, Wasserman SI, et al: Inhibition of neutrophil chemotaxis in association with experimental angioedema in patients with cold urticaria: A model of chemotactic deactivation in vivo . Clin Exp Immunol 1979;35:112-118. 19. Solomkin JS, Jenkins MK, Nelson RD, et al: Neutrophil dysfunction in sepsis: II. Evidence for the role of complement activation products in cellular deactivation . Surgery 1981;90:319-327. 20. Van Epps DE, Garcia ML: Enhancement of neutrophil function as a result of prior exposure to chemotactic factor . J Clin Invest 1980;66: 167-175.Crossref 21. Gallin JI, Wright DG, Schiffman E: Role of secretory events in modulating human neutrophil chemotaxis . J Clin Invest 1978;62:1364-1374.Crossref 22. Kay AB: Complement receptor enhancement by chemotactic factors . Mol Immunol 1982;19:1307-1311.Crossref 23. Fearon DT, Collins LA: Increased expression of C3b on polymorpho-nuclear leukocytes induced by chemotactic factors and by purification procedures . J Immunol 1983;130:370-375. 24. Schiffmann E, Corcoran BA, Wahl SM: N-formylmethionyl peptides as chemoattractants for leukocytes . Proc Natl Acad Sci USA 1975;72: 1059-1062.Crossref

Journal

Archives of SurgeryAmerican Medical Association

Published: Jan 1, 1987

References