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Increased Protocollagen Proline Hydroxylase Activity in Sclerodermatous Skin

Increased Protocollagen Proline Hydroxylase Activity in Sclerodermatous Skin Abstract Protocollagen proline hydroxylase (PPH) activity in skin was determined in vitro by radioassay employing a tritium-labeled L-proline-peptide substrate. Average PPH activity was significantly higher in biopsy specimens of both forearm and parasacral skin from 20 patients with scleroderma than in specimens from ten normal subjects and 17 patients with other diseases. These findings offer evidence that collagen synthesis is increased in the skin in scleroderma and suggest that assay of PPH activity in skin may be a useful tool for the diagnosis of this disease. References 1. Stein HD, Keiser HR, Sjoerdsma A: Collagen synthesis in granulating wounds of rats and man . Surg Forum 20:63-65, 1969. 2. Mussini E, Hutton JJ Jr, Udenfriend S: Collagen proline hydroxylase in wound healing, granuloma formation, scurvy, and growth . Science 157:927-929, 1967.Crossref 3. Takeuchi T, Prockop DJ: Protocollagen proline hydroxylase in normal liver and in hepatic fibrosis . Gastroenterology 56:744-750, 1969. 4. Uitto J, Halme J, Hannuksela M, et al: Protocollagen proline hydroxylase activity in the skin of normal human subjects and of patients with scleroderma . Scand J Clin Lab Invest 23:241-247, 1969.Crossref 5. Langner RO, Fuller GC: Elevation of proline hydroxylase activity in diseased rabbit aorta . Biochem Biophys Res Commun 36:559-566, 1969.Crossref 6. Halme J, Uitto J, Kahanpää K, et al: Protocollagen proline hydroxylase activity in experimental pulmonary fibrosis of rats . J Lab Clin Med 75:535-541, 1970. 7. Bianchi FA, Bistue AR, Wendt VE, et al: Analysis of 27 cases of progressive systemic sclerosis (including two with combined systemic lupus erythematosus) and a review of the literature . J Chronic Dis 19:953-977, 1966.Crossref 8. Laitinen O, Uitto J, Hannuksela M, et al: Solubility and turnover of collagen in collagen diseases . Ann Clin Res 1:64-73, 1969. 9. Harris ED Jr, Sjoerdsma A: Effect of penicillamine on human collagen and its possible application to treatment of scleroderma . Lancet 2:996-999, 1966.Crossref 10. Laitinen O, Uitto J, H[ill]nuksela M, et al: Increased soluble collagen content of affected and normal-looking skin in dermatomyositis, lupus erythematosus and scleroderma . Ann Med Exp Biol Fenn 44:507-509, 1966. 11. Keiser HR, Sjoerdsma A: Direct measurement of the rate of collagen synthesis in skin . Clin Chim Acta 23:341-346, 1969.Crossref 12. Hutton JJ Jr, Tappel AL, Udenfriend S: A rapid assay for collagen proline hydroxylase . Anal Biochem 16:384-394, 1966.Crossref 13. Hutton JJ Jr, Tappel AL, Undenfriend S: Cofactor and substrate requirements of collagen proline hydroxylase . Arch Biochem Biophys 118:231-240, 1967.Crossref 14. Stein HD, Keiser HR, Sjoerdsma A: Proline-hydroxylase activity in human blood . Lancet 1:106-109, 1970.Crossref 15. Rhoads RE, Hutton JJ Jr, Udenfriend S: Factors which stimulate collagen proline hydroxylase . Arch Biochem 122:805-807, 1967.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Dermatology American Medical Association

Increased Protocollagen Proline Hydroxylase Activity in Sclerodermatous Skin

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Publisher
American Medical Association
Copyright
Copyright © 1971 American Medical Association. All Rights Reserved.
ISSN
0003-987X
eISSN
1538-3652
DOI
10.1001/archderm.1971.04000190059008
Publisher site
See Article on Publisher Site

Abstract

Abstract Protocollagen proline hydroxylase (PPH) activity in skin was determined in vitro by radioassay employing a tritium-labeled L-proline-peptide substrate. Average PPH activity was significantly higher in biopsy specimens of both forearm and parasacral skin from 20 patients with scleroderma than in specimens from ten normal subjects and 17 patients with other diseases. These findings offer evidence that collagen synthesis is increased in the skin in scleroderma and suggest that assay of PPH activity in skin may be a useful tool for the diagnosis of this disease. References 1. Stein HD, Keiser HR, Sjoerdsma A: Collagen synthesis in granulating wounds of rats and man . Surg Forum 20:63-65, 1969. 2. Mussini E, Hutton JJ Jr, Udenfriend S: Collagen proline hydroxylase in wound healing, granuloma formation, scurvy, and growth . Science 157:927-929, 1967.Crossref 3. Takeuchi T, Prockop DJ: Protocollagen proline hydroxylase in normal liver and in hepatic fibrosis . Gastroenterology 56:744-750, 1969. 4. Uitto J, Halme J, Hannuksela M, et al: Protocollagen proline hydroxylase activity in the skin of normal human subjects and of patients with scleroderma . Scand J Clin Lab Invest 23:241-247, 1969.Crossref 5. Langner RO, Fuller GC: Elevation of proline hydroxylase activity in diseased rabbit aorta . Biochem Biophys Res Commun 36:559-566, 1969.Crossref 6. Halme J, Uitto J, Kahanpää K, et al: Protocollagen proline hydroxylase activity in experimental pulmonary fibrosis of rats . J Lab Clin Med 75:535-541, 1970. 7. Bianchi FA, Bistue AR, Wendt VE, et al: Analysis of 27 cases of progressive systemic sclerosis (including two with combined systemic lupus erythematosus) and a review of the literature . J Chronic Dis 19:953-977, 1966.Crossref 8. Laitinen O, Uitto J, Hannuksela M, et al: Solubility and turnover of collagen in collagen diseases . Ann Clin Res 1:64-73, 1969. 9. Harris ED Jr, Sjoerdsma A: Effect of penicillamine on human collagen and its possible application to treatment of scleroderma . Lancet 2:996-999, 1966.Crossref 10. Laitinen O, Uitto J, H[ill]nuksela M, et al: Increased soluble collagen content of affected and normal-looking skin in dermatomyositis, lupus erythematosus and scleroderma . Ann Med Exp Biol Fenn 44:507-509, 1966. 11. Keiser HR, Sjoerdsma A: Direct measurement of the rate of collagen synthesis in skin . Clin Chim Acta 23:341-346, 1969.Crossref 12. Hutton JJ Jr, Tappel AL, Udenfriend S: A rapid assay for collagen proline hydroxylase . Anal Biochem 16:384-394, 1966.Crossref 13. Hutton JJ Jr, Tappel AL, Undenfriend S: Cofactor and substrate requirements of collagen proline hydroxylase . Arch Biochem Biophys 118:231-240, 1967.Crossref 14. Stein HD, Keiser HR, Sjoerdsma A: Proline-hydroxylase activity in human blood . Lancet 1:106-109, 1970.Crossref 15. Rhoads RE, Hutton JJ Jr, Udenfriend S: Factors which stimulate collagen proline hydroxylase . Arch Biochem 122:805-807, 1967.Crossref

Journal

Archives of DermatologyAmerican Medical Association

Published: Jul 1, 1971

References