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In Vitro Evaluation of Inhibitors of Platelet Release and Aggregation

In Vitro Evaluation of Inhibitors of Platelet Release and Aggregation Abstract In order to determine which drugs would be most effective as inhibitors of platelet release and aggregation, In vitro release reactions and platelet aggregometry were used to evaluate aspirin, dipyridamole, sulfinpyrazone, flurbiprofen, low molecular weight dextran (dextran 40), prostaglandin E, (PGE1), apyrase, and adenosine. Adenosine diphosphate-induced aggregation was most effectively inhibited by PGF1, sulfinpyrazone, and dipyridamole. The latter had to be used in large doses. Collagen and epinephrine-induced release and aggregation were inhibited by the same drugs as well as by aspirin and apyrase. Antithynocyte globulin (ATG)-lnduced release and aggregation could only be partially blocked by these agents. In vitro studies suggest that sulfinpyrazone is one of the most effective of platelet inhibitors currently available for clinical testing. References 1. Mustard JF: Platelets in thrombosis: Mechanisms and therapy . Hosp Pract 4:46-56, 1969. 2. Rosenberg JC, Hawkins E, Rector F: Mechanisms of immunological injury during antibody-mediated hyperacute rejection of renal heterografts . Transplantation 11:151-157, 1971.Crossref 3. Broersma R, Mammen E, Steiman R, et al: Detection of antibodies to transplantation antigens by platelet aggregometry . J Surg Res 11:173-178, 1971.Crossref 4. Rosenberg JC, Lekas N, Lysz K, et al: Effect of antithymocyte globulin and other immune reactants on human platelets. Surgery 77:520-529, 1975. 5. Weiss HJ: The pharmacology of platelet inhibitor . Prog Hemostasis Thromb 1:199-231, 1972. 6. Salzman EW, Weisenberger H: Role of cyclic AMP in platelet function . Adv Cyclic Nucleotide Res 1:231-247, 1972. 7. Sullivan JM, Harken DE, Gorlin R: Pharmacologic control of thromboembolic complications of cardiac value replacement . N Engl J Med 284:1391-1394, 1971.Crossref 8. Mathew TH, Clyne DH, Nanra RS, et al: Controlled trial of oral anticoagulants and dipyridamole in cadaveric renal allografts . Lancet 1:1307-1310, 1974.Crossref 9. Kalgi A, Pineo GF, Shimizu A, et al: Arteriovenous shunt thrombosis: Prevention by sulfinpyrazone . New Engl J Med 290:304-306, 1974.Crossref 10. Elwood PC, Cochrane AL, Burr ML, et al: Randomized controlled trial of acetyl-salicylic acid in secondary prevention of mortality from myocardial infarction . Br Med J 1:436-440, 1974.Crossref 11. Harrison MJG, Marshall J, Meadows JC, et al: Effect of aspirin in amaurosis fugax . Lancet 2:743-744, 1971.Crossref 12. Salzman EW, Harris WH, DeSanctis RW: Reduction in venous thromboembolism by agents affecting platelet function . New Engl J Med 284:1287-1292, 1971.Crossref 13. Thomas J, Hume DM: A standardized ALG for use in man . Transplant Proc 4:477-479, 1972. 14. Emmons PR, Harrison JJG, Honour AJ, et al: Effect of dipyridamole on human platelet behaviour . Lancet 2:603-606, 1965.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Surgery American Medical Association

In Vitro Evaluation of Inhibitors of Platelet Release and Aggregation

Archives of Surgery , Volume 110 (8) – Aug 1, 1975

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Publisher
American Medical Association
Copyright
Copyright © 1975 American Medical Association. All Rights Reserved.
ISSN
0004-0010
eISSN
1538-3644
DOI
10.1001/archsurg.1975.01360140124024
Publisher site
See Article on Publisher Site

Abstract

Abstract In order to determine which drugs would be most effective as inhibitors of platelet release and aggregation, In vitro release reactions and platelet aggregometry were used to evaluate aspirin, dipyridamole, sulfinpyrazone, flurbiprofen, low molecular weight dextran (dextran 40), prostaglandin E, (PGE1), apyrase, and adenosine. Adenosine diphosphate-induced aggregation was most effectively inhibited by PGF1, sulfinpyrazone, and dipyridamole. The latter had to be used in large doses. Collagen and epinephrine-induced release and aggregation were inhibited by the same drugs as well as by aspirin and apyrase. Antithynocyte globulin (ATG)-lnduced release and aggregation could only be partially blocked by these agents. In vitro studies suggest that sulfinpyrazone is one of the most effective of platelet inhibitors currently available for clinical testing. References 1. Mustard JF: Platelets in thrombosis: Mechanisms and therapy . Hosp Pract 4:46-56, 1969. 2. Rosenberg JC, Hawkins E, Rector F: Mechanisms of immunological injury during antibody-mediated hyperacute rejection of renal heterografts . Transplantation 11:151-157, 1971.Crossref 3. Broersma R, Mammen E, Steiman R, et al: Detection of antibodies to transplantation antigens by platelet aggregometry . J Surg Res 11:173-178, 1971.Crossref 4. Rosenberg JC, Lekas N, Lysz K, et al: Effect of antithymocyte globulin and other immune reactants on human platelets. Surgery 77:520-529, 1975. 5. Weiss HJ: The pharmacology of platelet inhibitor . Prog Hemostasis Thromb 1:199-231, 1972. 6. Salzman EW, Weisenberger H: Role of cyclic AMP in platelet function . Adv Cyclic Nucleotide Res 1:231-247, 1972. 7. Sullivan JM, Harken DE, Gorlin R: Pharmacologic control of thromboembolic complications of cardiac value replacement . N Engl J Med 284:1391-1394, 1971.Crossref 8. Mathew TH, Clyne DH, Nanra RS, et al: Controlled trial of oral anticoagulants and dipyridamole in cadaveric renal allografts . Lancet 1:1307-1310, 1974.Crossref 9. Kalgi A, Pineo GF, Shimizu A, et al: Arteriovenous shunt thrombosis: Prevention by sulfinpyrazone . New Engl J Med 290:304-306, 1974.Crossref 10. Elwood PC, Cochrane AL, Burr ML, et al: Randomized controlled trial of acetyl-salicylic acid in secondary prevention of mortality from myocardial infarction . Br Med J 1:436-440, 1974.Crossref 11. Harrison MJG, Marshall J, Meadows JC, et al: Effect of aspirin in amaurosis fugax . Lancet 2:743-744, 1971.Crossref 12. Salzman EW, Harris WH, DeSanctis RW: Reduction in venous thromboembolism by agents affecting platelet function . New Engl J Med 284:1287-1292, 1971.Crossref 13. Thomas J, Hume DM: A standardized ALG for use in man . Transplant Proc 4:477-479, 1972. 14. Emmons PR, Harrison JJG, Honour AJ, et al: Effect of dipyridamole on human platelet behaviour . Lancet 2:603-606, 1965.Crossref

Journal

Archives of SurgeryAmerican Medical Association

Published: Aug 1, 1975

References