Abstract An indirect immunoperoxidase staining method has been successfully applied to 4% formaldehyde solution tissue sections fixed in and embedded in paraffin for the localization of Sporothrix schenckii and Cryptococcus neoformans without prior trypsinization of tissue sections. A comparison of this method with an analogous immunofluorescence staining technique has been made. (Arch Dermatol 115:433-435, 1979) References 1. Kaplan W, Kraft DE: Demonstration of pathogenic fungi in formalin-fixed tissue by immunofluorescence . Am J Clin Pathol 52:420-432, 1969. 2. Taylor CR, Burns J: The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labeled antibody . J Clin Pathol 27:14-20, 1974.Crossref 3. Nakane PK, Pierce BG: Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens . J Cell Biol 33:307-318, 1967.Crossref 4. Beckett JH, Bigbee JW: Immunoperoxidase localization of Treponema pallidum: Its use in formaldehyde-fixed and paraffin-embedded tissue sections . Arch Pathol 103:135-138, 1979. 5. Vogel RA: The indirect fluorescent antibody test for the detection of antibody in human cryptococcal disease . J Infect Dis 116:573-580, 1966.Crossref 6. Kaufman L, Blumer S: Value and interpretation of serological tests for the diagnosis of cryptococcosis . Appl Microbiol 16:1907-1909, 1968. 7. Wolf P, Rusell B, Jacobs PH: Immunofluorescence as a diagnostic aid in cryptococcal meningitis . Hum Pathol 5:758-760, 1974.Crossref 8. Burns J: Background staining and sensitivity of the unlabeled antibody-enzyme (PAP) method: Comparison with the peroxidase-labeled antibody sandwich method using formalin-fixed paraffin-embedded material . Histochemistry 43:291-294, 1975.Crossref 9. Petts V, Roitt IM: Peroxidase conjugates for demonstration of tissue antibodies: Evaluation of the technique . Clin Exp Immunol 9:407-418, 1971. 10. Kaufman L: Serology: Its value in the diagnosis of coccidioidomycosis cryptococcosis, and histoplasmosis , in Proceedings of the International Symposium on Mycoses , Pan American Health Organization scientific publication No. 205. Washington, DC, World Health Organization, 1970, pp 96-100.
Archives of Dermatology – American Medical Association
Published: Apr 1, 1979
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