Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You and Your Team.

Learn More →

Immunohistochemical Evaluation of Conjunctival Fibrillin-1 in Marfan Syndrome

Immunohistochemical Evaluation of Conjunctival Fibrillin-1 in Marfan Syndrome CLINICAL SCIENCES Immunohistochemical Evaluation of Conjunctival Fibrillin-1 in Marfan Syndrome Anuradha Ganesh, MD; Charles Smith, MD; Wilson Chan, MSc; Sheila Unger, MD; Nada Quercia, MSc; Maurice Godfrey, PhD; Stephen Kraft, MD; Raymond Buncic, MD; Alex Levin, MD Objective: To evaluate status of conjunctival fibrillin-1 rounding collagen bundles. The fresh frozen samples from in patients with Marfan syndrome with ectopia lentis. patients with Marfan syndrome showed consistent quali- tative differences in fibrillin-1 staining when compared Methods: Frozen sections of conjunctiva from 6 pa- with samples from control subjects. The fibrils were longer tients with Marfan syndrome with ectopia lentis and from and straighter than normal, varied in caliber, and showed 15 age-matched control subjects were stained with mouse fewer tendencies to form a woven pattern. antihuman fibrillin-1 antibody, using an avidin biotin im- munoperoxidase technique. The fibrillin-1 staining char- Conclusions: Consistent, qualitative abnormalities in acteristics of conjunctiva were analyzed with the light mi- fibrillin-1 staining pattern can be seen in the conjunc- croscope. tiva of patients with Marfan syndrome with ectopia len- tis. Conjunctival biopsy deserves further investigation as Results: All the fresh frozen sections of conjunctival a diagnostic modality for Marfan syndrome in patients samples from control subjects demonstrated http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png JAMA Ophthalmology American Medical Association

Immunohistochemical Evaluation of Conjunctival Fibrillin-1 in Marfan Syndrome

Loading next page...
 
/lp/american-medical-association/immunohistochemical-evaluation-of-conjunctival-fibrillin-1-in-marfan-d1HQ80g04q
Publisher
American Medical Association
Copyright
Copyright 2006 American Medical Association. All Rights Reserved. Applicable FARS/DFARS Restrictions Apply to Government Use.
ISSN
2168-6165
eISSN
2168-6173
DOI
10.1001/archopht.124.2.205
pmid
16476890
Publisher site
See Article on Publisher Site

Abstract

CLINICAL SCIENCES Immunohistochemical Evaluation of Conjunctival Fibrillin-1 in Marfan Syndrome Anuradha Ganesh, MD; Charles Smith, MD; Wilson Chan, MSc; Sheila Unger, MD; Nada Quercia, MSc; Maurice Godfrey, PhD; Stephen Kraft, MD; Raymond Buncic, MD; Alex Levin, MD Objective: To evaluate status of conjunctival fibrillin-1 rounding collagen bundles. The fresh frozen samples from in patients with Marfan syndrome with ectopia lentis. patients with Marfan syndrome showed consistent quali- tative differences in fibrillin-1 staining when compared Methods: Frozen sections of conjunctiva from 6 pa- with samples from control subjects. The fibrils were longer tients with Marfan syndrome with ectopia lentis and from and straighter than normal, varied in caliber, and showed 15 age-matched control subjects were stained with mouse fewer tendencies to form a woven pattern. antihuman fibrillin-1 antibody, using an avidin biotin im- munoperoxidase technique. The fibrillin-1 staining char- Conclusions: Consistent, qualitative abnormalities in acteristics of conjunctiva were analyzed with the light mi- fibrillin-1 staining pattern can be seen in the conjunc- croscope. tiva of patients with Marfan syndrome with ectopia len- tis. Conjunctival biopsy deserves further investigation as Results: All the fresh frozen sections of conjunctival a diagnostic modality for Marfan syndrome in patients samples from control subjects demonstrated

Journal

JAMA OphthalmologyAmerican Medical Association

Published: Feb 1, 2006

References