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Immune Responses in Rabbits With Phlyctenules and Catarrhal Infiltrates

Immune Responses in Rabbits With Phlyctenules and Catarrhal Infiltrates Abstract • Corneal phlyctenules and catarrhal infiltrates developed in rabbits immunized with Staphylococcus aureus cell walls (CWs) mixed with complete Freund's adjuvant (CFA) after topical challenge with viable S aureus. The immune responses of these rabbits to CWs and ribitol teichoic acid (RTA) before and after immunization with CW/CFA were studied and the results of skin tests with lymphocyte blastogenesis and hemagglutination tests correlated. After immunization of rabbits with CW/CFA, their lymphocytes showed a greater proliferative response to CWs in the lymphocyte blastogenesis test that correlated with delayed hypersensitivity reactions found in skin tests. Both tests suggested that the lymphocytes of immunized rabbits showed cellular immunity to CWs. Skin tests to RTA suggested an Arthus but not a delayed hypersensitivity reaction to this antigen. The results of skin tests and hemagglutination tests suggested that immunized rabbits expressed humoral and immunity to RTA, the major antigenic determinant of S aureus. References 1. Mondino BJ, Kowalski R, Ratajczak HV, et al: Rabbit model of phlyctenulosis and catarrhal infiltrates . Arch Ophthalmol 1981;99:891-895.Crossref 2. Joklik WK, Willett HP, Amos DB: Zinsser Microbiology , ed 17. New York, Appleton-Century-Crofts, 1980, pp 535-540. 3. Mondino BJ, Kowalski R: Phlyctenules and catarrhal infiltrates in rabbits immunized with staphylococcal cell walls . Arch Ophthalmol 1982;100:1968-1971.Crossref 4. Dziarski R: Teichoic acids . Curr Top Microbiol Immunol 1976;74:113-135. 5. Dziarski R, Dziarski A, Levinson AI: Mitogenic responsiveness of mouse, cat and human lymphocytes to Staphylococcus aureus cell wall, teichoic acid, and peptidoglycan . Int Arch Allergy Appl Immunol 1980;63:383-395.Crossref 6. Cornu P, Gratwohl A, Schmid E, et al: A simple technique for testing the in vitro response of rabbit lymphocytes to PHA and allogeneic cells . Experientia 1979;35:281-283.Crossref 7. Brock JH, Reiter B: Sensitization of sheep erythrocytes by cell wall teichoic acid of Staphylococcus aureus . Immunochemistry 1971;8:933-938.Crossref 8. Heczko PB, Grov A, Oeding P: In vivo reactions of staphylococcal antigens . Acta Pathol Microbiol Scand B 1973;81:732-740. 9. Lillie RD: Histopathologic Technic and Practical Histochemistry , ed 2. New York, McGraw-Hill Book Co, 1954, pp 303-304. 10. Neta R, Salvin SB, Sabaawi M: Mechanisms in the in vivo release of lymphokines . Cell Immunol 1981;64:203-219.Crossref 11. Kirkpatrick CH, Rich RR, Bennett JE: Chronic mucocutaneous candidiasis: Modelbuilding in cellular immunity . Ann Intern Med 1971;74:955-978.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

Immune Responses in Rabbits With Phlyctenules and Catarrhal Infiltrates

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Publisher
American Medical Association
Copyright
Copyright © 1983 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1983.01040020277022
Publisher site
See Article on Publisher Site

Abstract

Abstract • Corneal phlyctenules and catarrhal infiltrates developed in rabbits immunized with Staphylococcus aureus cell walls (CWs) mixed with complete Freund's adjuvant (CFA) after topical challenge with viable S aureus. The immune responses of these rabbits to CWs and ribitol teichoic acid (RTA) before and after immunization with CW/CFA were studied and the results of skin tests with lymphocyte blastogenesis and hemagglutination tests correlated. After immunization of rabbits with CW/CFA, their lymphocytes showed a greater proliferative response to CWs in the lymphocyte blastogenesis test that correlated with delayed hypersensitivity reactions found in skin tests. Both tests suggested that the lymphocytes of immunized rabbits showed cellular immunity to CWs. Skin tests to RTA suggested an Arthus but not a delayed hypersensitivity reaction to this antigen. The results of skin tests and hemagglutination tests suggested that immunized rabbits expressed humoral and immunity to RTA, the major antigenic determinant of S aureus. References 1. Mondino BJ, Kowalski R, Ratajczak HV, et al: Rabbit model of phlyctenulosis and catarrhal infiltrates . Arch Ophthalmol 1981;99:891-895.Crossref 2. Joklik WK, Willett HP, Amos DB: Zinsser Microbiology , ed 17. New York, Appleton-Century-Crofts, 1980, pp 535-540. 3. Mondino BJ, Kowalski R: Phlyctenules and catarrhal infiltrates in rabbits immunized with staphylococcal cell walls . Arch Ophthalmol 1982;100:1968-1971.Crossref 4. Dziarski R: Teichoic acids . Curr Top Microbiol Immunol 1976;74:113-135. 5. Dziarski R, Dziarski A, Levinson AI: Mitogenic responsiveness of mouse, cat and human lymphocytes to Staphylococcus aureus cell wall, teichoic acid, and peptidoglycan . Int Arch Allergy Appl Immunol 1980;63:383-395.Crossref 6. Cornu P, Gratwohl A, Schmid E, et al: A simple technique for testing the in vitro response of rabbit lymphocytes to PHA and allogeneic cells . Experientia 1979;35:281-283.Crossref 7. Brock JH, Reiter B: Sensitization of sheep erythrocytes by cell wall teichoic acid of Staphylococcus aureus . Immunochemistry 1971;8:933-938.Crossref 8. Heczko PB, Grov A, Oeding P: In vivo reactions of staphylococcal antigens . Acta Pathol Microbiol Scand B 1973;81:732-740. 9. Lillie RD: Histopathologic Technic and Practical Histochemistry , ed 2. New York, McGraw-Hill Book Co, 1954, pp 303-304. 10. Neta R, Salvin SB, Sabaawi M: Mechanisms in the in vivo release of lymphokines . Cell Immunol 1981;64:203-219.Crossref 11. Kirkpatrick CH, Rich RR, Bennett JE: Chronic mucocutaneous candidiasis: Modelbuilding in cellular immunity . Ann Intern Med 1971;74:955-978.Crossref

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Aug 1, 1983

References