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Immune Adherence Hemagglutination for Detection of Australia Antigen

Immune Adherence Hemagglutination for Detection of Australia Antigen Abstract The phenomenon of immune adherence was first described by Lavern and Mesnil1 and Levaditi2 at the beginning of this century. In 1953, Nelson3 defined the general principle that antigen-antibody complement complex adheres to primate erythrocytes or to nonprimate platelets and postulated the concept of immune adherence for this fundamental immunological phenomenon. Immune adherence hemagglutination technique (IAHA) and the participation of complement components in this reaction were described by Nishioka (1963)4 and by Nishioka and Linscott (1963).1 We found that the first four complement components, ie, Cl, C4, C2, and C3 reacted with antigen-antibody complex which resulted in positive adherence of human erythrocytes. This finding led to the discovery of other complement components required for immune hemolysis. If constant amounts of any two of the three variables (antigen, antibody, and complement) are employed, the third variable can be quantitated. This method measures as little as References 1. Laveran A, Mesnil F: Recherches morphologiques et expérimentales sur le trypanosome des rats . Ann Inst Pasteur 15:673-714, 1901. 2. Levaditi C: Sur l'etat de la cytase dans le plasma des animaux normaux et des organismes vaccinés contre le vibrion cholérique . Ann Inst Pasteur 15:894-927, 1901. 3. Nelson RA Jr: The immune adherence phenomenon: An immunologically specific reaction between microorganisms and erythrocytes leading to enhanced phagocytosis . Science 118:733-737, 1953.Crossref 4. Nishioka K: Measurement of complement by agglutination of human erythrocytes reacting in immune adherence . J Immun 90:86-97, 1963. 5. Nishioka K, Linscott WD: Components of guinea pig complement: I. Separation of a serum fraction essential for immune hemolysis and immune adherence . J Exp Med 118:767-793, 1963.Crossref 6. Okochi K, Mayumi M, Haguino Y, et al: Evaluation of frequency of Australia antigen in blood donors of Tokyo by means of immune adherence hemagglutination technique . Vox Sang 19:332-337, 1970.Crossref 7. Nishioka K, Tachibana T, Stock CC: Detection of antibody and antigen by immune adherence , in Ito Y (ed): Monograph of the 1st International Symposium on Tumor Viruses . Nagoya, Japan, 1967, pp 253-264. 8. Nishioka K: Complement and tumor immunology . Advances Cancer Res 14:231-293, 1971. 9. Ito M, Tagaya I: Immune adherence hemagglutination test as a new sensitive method for titration of animal virus antigens and antibodies . Japan J Med Sci 19:109-126, 1966. 10. Mayumi M, Okochi K, Nishioka K: Detection of Australia a antigen by means of immune adherence hemagglutination test . Vox Sang 20:178-181, 1971.Crossref 11. Tamura N, Nelson RA Jr: Three naturally-occuring inhibitors of components of complement guinea pig and rabbit serum . J Immun 99:582-589, 1967. 12. Nishioka K: Complement and immune complex. Molecular anatomy of C3 on antigen antibody complex with special references to immune adherence reactivity , in Proceedings of the VII International Congress Allergology, Florence 1970. Excerpta Medica 7:259-267, 1971. 13. Simons MJ, Yap EH, Ong YW, et al: Frequency of Australia antigen in blood donors in Singapore . Amer J Dis Child 123:358-359, 1972. 14. Obayashi A, Mayumi M, Okochi K: Australia antigen in antigen in familiar cirrhosis . Lancet 1:244, 1971.Crossref http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Diseases of Children American Medical Association

Immune Adherence Hemagglutination for Detection of Australia Antigen

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Publisher
American Medical Association
Copyright
Copyright © 1972 American Medical Association. All Rights Reserved.
ISSN
0002-922X
DOI
10.1001/archpedi.1972.02110100138052
Publisher site
See Article on Publisher Site

Abstract

Abstract The phenomenon of immune adherence was first described by Lavern and Mesnil1 and Levaditi2 at the beginning of this century. In 1953, Nelson3 defined the general principle that antigen-antibody complement complex adheres to primate erythrocytes or to nonprimate platelets and postulated the concept of immune adherence for this fundamental immunological phenomenon. Immune adherence hemagglutination technique (IAHA) and the participation of complement components in this reaction were described by Nishioka (1963)4 and by Nishioka and Linscott (1963).1 We found that the first four complement components, ie, Cl, C4, C2, and C3 reacted with antigen-antibody complex which resulted in positive adherence of human erythrocytes. This finding led to the discovery of other complement components required for immune hemolysis. If constant amounts of any two of the three variables (antigen, antibody, and complement) are employed, the third variable can be quantitated. This method measures as little as References 1. Laveran A, Mesnil F: Recherches morphologiques et expérimentales sur le trypanosome des rats . Ann Inst Pasteur 15:673-714, 1901. 2. Levaditi C: Sur l'etat de la cytase dans le plasma des animaux normaux et des organismes vaccinés contre le vibrion cholérique . Ann Inst Pasteur 15:894-927, 1901. 3. Nelson RA Jr: The immune adherence phenomenon: An immunologically specific reaction between microorganisms and erythrocytes leading to enhanced phagocytosis . Science 118:733-737, 1953.Crossref 4. Nishioka K: Measurement of complement by agglutination of human erythrocytes reacting in immune adherence . J Immun 90:86-97, 1963. 5. Nishioka K, Linscott WD: Components of guinea pig complement: I. Separation of a serum fraction essential for immune hemolysis and immune adherence . J Exp Med 118:767-793, 1963.Crossref 6. Okochi K, Mayumi M, Haguino Y, et al: Evaluation of frequency of Australia antigen in blood donors of Tokyo by means of immune adherence hemagglutination technique . Vox Sang 19:332-337, 1970.Crossref 7. Nishioka K, Tachibana T, Stock CC: Detection of antibody and antigen by immune adherence , in Ito Y (ed): Monograph of the 1st International Symposium on Tumor Viruses . Nagoya, Japan, 1967, pp 253-264. 8. Nishioka K: Complement and tumor immunology . Advances Cancer Res 14:231-293, 1971. 9. Ito M, Tagaya I: Immune adherence hemagglutination test as a new sensitive method for titration of animal virus antigens and antibodies . Japan J Med Sci 19:109-126, 1966. 10. Mayumi M, Okochi K, Nishioka K: Detection of Australia a antigen by means of immune adherence hemagglutination test . Vox Sang 20:178-181, 1971.Crossref 11. Tamura N, Nelson RA Jr: Three naturally-occuring inhibitors of components of complement guinea pig and rabbit serum . J Immun 99:582-589, 1967. 12. Nishioka K: Complement and immune complex. Molecular anatomy of C3 on antigen antibody complex with special references to immune adherence reactivity , in Proceedings of the VII International Congress Allergology, Florence 1970. Excerpta Medica 7:259-267, 1971. 13. Simons MJ, Yap EH, Ong YW, et al: Frequency of Australia antigen in blood donors in Singapore . Amer J Dis Child 123:358-359, 1972. 14. Obayashi A, Mayumi M, Okochi K: Australia antigen in antigen in familiar cirrhosis . Lancet 1:244, 1971.Crossref

Journal

American Journal of Diseases of ChildrenAmerican Medical Association

Published: Apr 1, 1972

References