Abstract • Twenty-eight adult dogs underwent thoracoabdominal bypass with 6-mm double-velour Dacron grafts. The experimental grafts were seeded immediately prior to implantation with enzymatically harvested endothelial cells. Endothelial cells were procured from autologous external jugular vein segments using 0.1% trypsin and 0.5% collagenase solutions. Unseeded grafts served as controls. The grafts were studied from one to 28 days after implantation. The seeded grafts exhibited greater than 80% uniform luminal coverage with endothelial cells at day 28. Immunofluorescent microscopy was used to confirm the presence of endothelium. The experimental grafts studied at the two time periods of one to seven days and 14 to 28 days had 94.6% and 88.5% clot-free surfaces, respectively. The control grafts studied during similar periods had 81.0% and 40.1% clot-free surfaces. These differences were significant at the 14- to 28–day period. (Arch Surg 115:1289-1294, 1980) References 1. Graham LM, Vinter DW, Ford JW, et al: Cultured autogenous endothelial cell seeding of prosthetic vascular grafts . Surg Forum 30:204-206, 1979. 2. Graham LM, Vinter DW, Ford JW, et al: Endothelial cell seeding of prosthetic vascular grafts: Early experimental studies with cultured autologous canine endothelium . Arch Surg 115:929-933, 1980.Crossref 3. Ford JW, Burkel WE, Kahn RH: Isolation of adult canine venous endothelium for tissue culture. In Vitro, to be published. 4. Yates SG, Barros D'Sa AAB, Berger KE, et al: The preclotting of porous arterial prostheses . Ann Surg 188:611-622, 1978.Crossref 5. Benson RE, Dodds WJ: Immunologic characterization of canine factor VIII . Blood 48:521-529, 1976. 6. Jaffe EA, Hoyer LW, Nachman RL: Synthesis of antihemophilic factor antigen by cultured human endothelial cells . J Clin Invest 522757-2764, 1973.Crossref 7. Dilley R, Herring M, Boxer L, et al: Immunofluorescent staining for factor VIII-related antigens: A tool for study of healing in vascular prostheses . J Surg Res 27:149-155, 1979.Crossref 8. Herring HB, Dilley R, Hersilde RA Jr, et al: Seeding arterial prostheses with vascular endothelium: The nature of the lining . Ann Surg 190:84-90, 1979.Crossref 9. Herring M, Gardner A, Glover J: A single-staged technique for seeding vascular grafts with autogenous endothelium . Surgery 84:498-504, 1978. 10. Herring M, Gardner A, Glover J: Seeding endothelium onto canine arterial prostheses: The effects of graft design . Arch Surg 114:679-682, 1979.Crossref 11. Herring M, Dilley R, Cullison T, et al: Seeding endothelium on canine arterial prostheses: The size of the inoculum . J Surg Res 28:35-38, 1980.Crossref 12. Burkel WE, Kahn RH, Stanley JC: Development and Testing of Pseudointimal-lined Vascular Devices , National Heart, Lung, and Blood Institute contract NIH-N01-HV-2054. Springfield, Va, National Technical Information Service, 1979. 13. Swartz SM, Selden SC III, Bowman P: Growth control in aortic endothelium at wound edges , in Sato GH, Ross R (eds): Hormones and Cell Cultures . Cold Springs Harbor, NY, Cold Springs Harbor Laboratory, 1979, pp 593-610.
Archives of Surgery – American Medical Association
Published: Nov 1, 1980