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By means of the precipitin reaction, urinary proteins in nephritis have been studied and found to consist of the following blood proteins: albumin, pseudoglobulin and euglobulin. These proteins vary in their quantitative relationship in different individuals. When the method of globular crystallization with subsequent dialysis of the solution of the crystals was applied to the urinary proteins and the precipitin reaction applied to the successive fractions, the latter ones gave less response to the serum of definite titer than a blood albumin solution of the same percentage content. In one case a fraction of this character was obtained that showed a precipitin reaction only in high concentration with a powerful antialbumin serum. The same protein, however, when injected, produced an antialbumin serum. In the search for nonblood proteins characteristic of a definite condition, it was found that in experimental uremia produced by hypertonic sodium chloride and in both canine and
JAMA – American Medical Association
Published: Nov 17, 1928
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