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Identification of Group A Streptococci: Bacitracin Disc and Fluorescent Antibody Techniques Compared with the Lancefield Precipitin Method

Identification of Group A Streptococci: Bacitracin Disc and Fluorescent Antibody Techniques... Abstract During the course of a project which had as its primary objective the evaluation of a streptococcal disease control program,1 an opportunity was presented to compare the efficacy of (a) the bacitracin disc method and (b) the fluorescent antibody method of identifying Group A streptococci with the Lancefield precipitin technique. The results which were obtained and are here presented are confirmatory of previous work2,3 and show that both the bacitracin disc and the fluorescent antibody techniques are highly reliable. Each, therefore, represents a useful method for the identification of Group A streptococci in clinical laboratories. Methods A total of 776 strains of streptococci was subjected to study; 669 (90%) were isolated from patients who were seen on the clinical services of the Denver General Hospital, while 69 strains (10%) were obtained from household contacts of patients included in the study. Of the streptococcal isolations, 76% were made from References 1. Streamer, C. W., et al.: To be published. 2. Maxted, W. R.: The Use of Bacitracin for Identifying Group A Haemolytic Streptococci , J. Clin. Path. 6:224, 1953.Crossref 3. Levinson, M. L., and Frank, P. F.: Differentiation of Group A from Other Beta Hemolytic Streptococci with Bacitracin , J. Bact. 69:284, 1955. 4. Lancefield, R. C.: Microprecipitin Technique for Classifying Hemolytic Streptococci and Improved Methods for Producing Anti-Sera , Proc. Soc. Exp. Biol. Med. 38:473, 1938.Crossref 5. Moody, M. D.; Ellis, E. C., and Updyke, E. L.: Staining Bacterial Smears with Fluorescent Antibody: IV. Grouping Streptococci with Fluorescent Antibody , J. Bact. 75:553, 1958. 6. Clifford, R., and Garrison, H. A.: Further Study of Differentiations of Group A Beta Hemolytic Streptococci with Bacitracin , Tomorrow's Scientists 3:2, 1958. 7. Peeples, W. J.; Spielman, D. W., and Moody, M. D.: Field Application of Fluorescent Antibody Technique for Identification of Group A Streptococcus , Public Health Rep. 76:651, 1961.Crossref 8. Warfield, M. A.; Page, R. H.; Zuelzer, W. W., and Stulberg, C. S.: Immunofluorescence in Diagnostic Bacteriology: II. Identification of Group A Streptococci in Throat Smears , Amer. J. Dis. Child. 101:160, 1961.Crossref 9. Redys, J. J.; Ross, M. R., and Borman, E. K.: Inhibition of Common-Antigen Fluorescence in Grouping Streptococci by the Fluorescent Antibody Method , J. Bact. 80:823, 1960. 10. Denny, F. W.; Wannamaker, L. W.; Boink, W. R.; Rammelkamp, C. H., Jr., and Custer, E. A.: Prevention of Rheumatic Fever , J.A.M.A. 143:151, 1950.Crossref 11. Chamovitz, R.; Catanzaro, F. J.; Stetson, C. A., and Rammelkamp, C. H., Jr.: Prevention of Rheumatic Fever by Treatment of Previous Streptococcal Infections , New Engl. J. Med. 251: 466, 1954.Crossref 12. Eagle, H.; Fleischman. R., and Musselman, A. D.: The Bactericidal Action of Penicillin in Vivo: The Participation of the Host, and the Slow Recovery of the Surviving Organisms , Ann. Intern. Med. 33:544, 1950.Crossref 13. Haight, T. H., and Finland, M.: Observations on the Mode of Action of Erythromycin , Proc. Soc. Exp. Biol. Med. 81:188, 1952.Crossref 14. Breese, B. B., and Disney, F. A.: The Accuracy of Diagnosis of Beta Streptococcal Infections on Clinical Grounds , J. Pediat. 44:670, 1954.Crossref 15. Coburn, A. F.: The Factor of Infection in the Rheumatic State , Baltimore, Williams & Wilkins Company, 1931. 16. Schlesinger, B.: The Relationship of Throat Infection to Acute Rheumatism in Childhood , Arch. Dis. Child. 5:411, 1930.Crossref 17. Swift, H. F.: The Relationship of Streptococcal Infections to Rheumatic Fever , Amer. J. Med. 2:168, 1947.Crossref 18. Rantz, L. A.; Boisvert, P. L., and Spink, W. W.: Etiology and Pathogenesis of Rheumatic Fever , Arch. Intern. Med. 76:131, 1945.Crossref 19. Glaser, R. J.: The Practical Approach to Streptococcal Infection in the Family with Special Reference to Culture Techniques, in Bulletin of the Denver Rheumatic Fever Diagnostic Service, Vol. 6, No. 4, 1960. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Diseases of Children American Medical Association

Identification of Group A Streptococci: Bacitracin Disc and Fluorescent Antibody Techniques Compared with the Lancefield Precipitin Method

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Publisher
American Medical Association
Copyright
Copyright © 1962 American Medical Association. All Rights Reserved.
ISSN
0002-922X
DOI
10.1001/archpedi.1962.02080030159008
Publisher site
See Article on Publisher Site

Abstract

Abstract During the course of a project which had as its primary objective the evaluation of a streptococcal disease control program,1 an opportunity was presented to compare the efficacy of (a) the bacitracin disc method and (b) the fluorescent antibody method of identifying Group A streptococci with the Lancefield precipitin technique. The results which were obtained and are here presented are confirmatory of previous work2,3 and show that both the bacitracin disc and the fluorescent antibody techniques are highly reliable. Each, therefore, represents a useful method for the identification of Group A streptococci in clinical laboratories. Methods A total of 776 strains of streptococci was subjected to study; 669 (90%) were isolated from patients who were seen on the clinical services of the Denver General Hospital, while 69 strains (10%) were obtained from household contacts of patients included in the study. Of the streptococcal isolations, 76% were made from References 1. Streamer, C. W., et al.: To be published. 2. Maxted, W. R.: The Use of Bacitracin for Identifying Group A Haemolytic Streptococci , J. Clin. Path. 6:224, 1953.Crossref 3. Levinson, M. L., and Frank, P. F.: Differentiation of Group A from Other Beta Hemolytic Streptococci with Bacitracin , J. Bact. 69:284, 1955. 4. Lancefield, R. C.: Microprecipitin Technique for Classifying Hemolytic Streptococci and Improved Methods for Producing Anti-Sera , Proc. Soc. Exp. Biol. Med. 38:473, 1938.Crossref 5. Moody, M. D.; Ellis, E. C., and Updyke, E. L.: Staining Bacterial Smears with Fluorescent Antibody: IV. Grouping Streptococci with Fluorescent Antibody , J. Bact. 75:553, 1958. 6. Clifford, R., and Garrison, H. A.: Further Study of Differentiations of Group A Beta Hemolytic Streptococci with Bacitracin , Tomorrow's Scientists 3:2, 1958. 7. Peeples, W. J.; Spielman, D. W., and Moody, M. D.: Field Application of Fluorescent Antibody Technique for Identification of Group A Streptococcus , Public Health Rep. 76:651, 1961.Crossref 8. Warfield, M. A.; Page, R. H.; Zuelzer, W. W., and Stulberg, C. S.: Immunofluorescence in Diagnostic Bacteriology: II. Identification of Group A Streptococci in Throat Smears , Amer. J. Dis. Child. 101:160, 1961.Crossref 9. Redys, J. J.; Ross, M. R., and Borman, E. K.: Inhibition of Common-Antigen Fluorescence in Grouping Streptococci by the Fluorescent Antibody Method , J. Bact. 80:823, 1960. 10. Denny, F. W.; Wannamaker, L. W.; Boink, W. R.; Rammelkamp, C. H., Jr., and Custer, E. A.: Prevention of Rheumatic Fever , J.A.M.A. 143:151, 1950.Crossref 11. Chamovitz, R.; Catanzaro, F. J.; Stetson, C. A., and Rammelkamp, C. H., Jr.: Prevention of Rheumatic Fever by Treatment of Previous Streptococcal Infections , New Engl. J. Med. 251: 466, 1954.Crossref 12. Eagle, H.; Fleischman. R., and Musselman, A. D.: The Bactericidal Action of Penicillin in Vivo: The Participation of the Host, and the Slow Recovery of the Surviving Organisms , Ann. Intern. Med. 33:544, 1950.Crossref 13. Haight, T. H., and Finland, M.: Observations on the Mode of Action of Erythromycin , Proc. Soc. Exp. Biol. Med. 81:188, 1952.Crossref 14. Breese, B. B., and Disney, F. A.: The Accuracy of Diagnosis of Beta Streptococcal Infections on Clinical Grounds , J. Pediat. 44:670, 1954.Crossref 15. Coburn, A. F.: The Factor of Infection in the Rheumatic State , Baltimore, Williams & Wilkins Company, 1931. 16. Schlesinger, B.: The Relationship of Throat Infection to Acute Rheumatism in Childhood , Arch. Dis. Child. 5:411, 1930.Crossref 17. Swift, H. F.: The Relationship of Streptococcal Infections to Rheumatic Fever , Amer. J. Med. 2:168, 1947.Crossref 18. Rantz, L. A.; Boisvert, P. L., and Spink, W. W.: Etiology and Pathogenesis of Rheumatic Fever , Arch. Intern. Med. 76:131, 1945.Crossref 19. Glaser, R. J.: The Practical Approach to Streptococcal Infection in the Family with Special Reference to Culture Techniques, in Bulletin of the Denver Rheumatic Fever Diagnostic Service, Vol. 6, No. 4, 1960.

Journal

American Journal of Diseases of ChildrenAmerican Medical Association

Published: Aug 1, 1962

References