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Human Ocular Mucus: Scanning Electron Microscopic Study

Human Ocular Mucus: Scanning Electron Microscopic Study Abstract • Twenty biopsy specimens of the human conjunctival epithelial surface with its associated mucus from normal subjects and contact lens wearers with biomicroscopic evidence of excess mucus were studied by scanning electron microscopy. Mucus existed in strands, sheets, and granules; each morphologic type of mucus was observed in all samples. No qualitative difference in morphologic features of mucus was found between normal subjects and those with excess mucus. Mucous forms observed by scanning electron microscopy closely resembled structures previously shown by biomicroscopic and light microscopic techniques, providing evidence that careful scanning electron microscopic preparation of biopsy specimens containing mucus may not greatly alter in situ morphologic characteristics of mucus. References 1. Kessing SV: Mucous gland system of conjunctiva: A quantitative normal anatomical study . Acta Ophthalmol , 1968, (suppl 95) , pp 1-133. 2. Greiner JV, Kenyon KR, Henriquez AS, et al: Mucus secretory vesicles in conjunctival epithelial cells of contact lens wearers . Arch Ophthalmol 1980;98:1843-1846.Crossref 3. Norn MS: Mucus on conjunctiva and cornea . Acta Ophthalmol 1963;41:13-24.Crossref 4. Adams AD: The morphology of human conjunctival mucus . Arch Ophthalmol 1979;97:730-734.Crossref 5. Greiner JV, Covington HI, Allansmith MR: Surface morphology of the human upper tarsal conjunctiva . Am J Ophthalmol 1977;83:892-905. 6. Greiner JV, Covington HI, Allansmith MR: The human limbus: A scanning electron microscopic study . Arch Ophthalmol 1979;97:1159-1165.Crossref 7. Greiner JV, Henriquez AS, Covington HI, et al: Goblet cells and crypts in the human conjunctiva . Arch Ophthalmol 1981;99:2190-2197.Crossref 8. Greiner JV, Allansmith MR: Effects of contact lens wear on the human conjunctival mucus system . Ophthalmology 1981;88:821-832.Crossref 9. Korb DR, Allansmith MR, Greiner JV, et al: Biomicroscopy of papillae associated with hard contact lens wearing . Ophthalmology 1981;88:1132-1136.Crossref 10. Holly FJ, Lemp MA: Wettability and wetting of the corneal epithelium . Exp Eye Res 1971;11:239-250.Crossref 11. Dudley JP, Cherry JD: Scanning electron microscopic demonstration of goblet cell discharge and mucous layer on nasal ciliated respiratory epithelium . Otolaryngol Head Neck Surg 1980;88:439-441. 12. Specian RD, Neutra MR: The surface topography of the colonic crypt in rabbit and monkey . Am J Anat 1981;160:461-472.Crossref 13. Chretien FC, Gernigon C, David G, et al: The ultrastructure of human cervical mucus under scanning electron microscopy . Fertil Steril 1973;24:746-757. 14. Knüsel O: Vitale Farbungen am menschlicken Auge: III. Morphologie der Schleimsekration der Konjunktiva . Z Augenheilkd 1923;51:257-270. 15. Bang BG, Bang FB: Responses of upper respiratory mucosae to dehydration and infection . Ann NY Acad Sci 1963;106:625-640.Crossref 16. Florey HW: Electron microscopic observations on goblet cells of the rat colon . Q J Exp Physiol 1960;45:329-336. 17. Shearman DJC, Muir AR: Observations on the secretory cycle of goblet cells . Q J Exp Physiol 1960;45:337-342. 18. Allansmith MR, Korb DR, Greiner JV, et al: Giant papillary conjunctivitis in contact lens wearers . Am J Ophthalmol 1977;83:697-708. 19. Fowler SA, Greiner JV, Allansmith MR: Soft contact lenses from patients with giant papillary conjunctivitis . Am J Ophthalmol 1979;88:1056-1061. 20. Norn MS: Ultrastructure of mucous thread in interior conjunctival fornix . Acta Ophthalmol 1967;45:727-732. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Ophthalmology American Medical Association

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Publisher
American Medical Association
Copyright
Copyright © 1982 American Medical Association. All Rights Reserved.
ISSN
0003-9950
eISSN
1538-3687
DOI
10.1001/archopht.1982.01030040592007
Publisher site
See Article on Publisher Site

Abstract

Abstract • Twenty biopsy specimens of the human conjunctival epithelial surface with its associated mucus from normal subjects and contact lens wearers with biomicroscopic evidence of excess mucus were studied by scanning electron microscopy. Mucus existed in strands, sheets, and granules; each morphologic type of mucus was observed in all samples. No qualitative difference in morphologic features of mucus was found between normal subjects and those with excess mucus. Mucous forms observed by scanning electron microscopy closely resembled structures previously shown by biomicroscopic and light microscopic techniques, providing evidence that careful scanning electron microscopic preparation of biopsy specimens containing mucus may not greatly alter in situ morphologic characteristics of mucus. References 1. Kessing SV: Mucous gland system of conjunctiva: A quantitative normal anatomical study . Acta Ophthalmol , 1968, (suppl 95) , pp 1-133. 2. Greiner JV, Kenyon KR, Henriquez AS, et al: Mucus secretory vesicles in conjunctival epithelial cells of contact lens wearers . Arch Ophthalmol 1980;98:1843-1846.Crossref 3. Norn MS: Mucus on conjunctiva and cornea . Acta Ophthalmol 1963;41:13-24.Crossref 4. Adams AD: The morphology of human conjunctival mucus . Arch Ophthalmol 1979;97:730-734.Crossref 5. Greiner JV, Covington HI, Allansmith MR: Surface morphology of the human upper tarsal conjunctiva . Am J Ophthalmol 1977;83:892-905. 6. Greiner JV, Covington HI, Allansmith MR: The human limbus: A scanning electron microscopic study . Arch Ophthalmol 1979;97:1159-1165.Crossref 7. Greiner JV, Henriquez AS, Covington HI, et al: Goblet cells and crypts in the human conjunctiva . Arch Ophthalmol 1981;99:2190-2197.Crossref 8. Greiner JV, Allansmith MR: Effects of contact lens wear on the human conjunctival mucus system . Ophthalmology 1981;88:821-832.Crossref 9. Korb DR, Allansmith MR, Greiner JV, et al: Biomicroscopy of papillae associated with hard contact lens wearing . Ophthalmology 1981;88:1132-1136.Crossref 10. Holly FJ, Lemp MA: Wettability and wetting of the corneal epithelium . Exp Eye Res 1971;11:239-250.Crossref 11. Dudley JP, Cherry JD: Scanning electron microscopic demonstration of goblet cell discharge and mucous layer on nasal ciliated respiratory epithelium . Otolaryngol Head Neck Surg 1980;88:439-441. 12. Specian RD, Neutra MR: The surface topography of the colonic crypt in rabbit and monkey . Am J Anat 1981;160:461-472.Crossref 13. Chretien FC, Gernigon C, David G, et al: The ultrastructure of human cervical mucus under scanning electron microscopy . Fertil Steril 1973;24:746-757. 14. Knüsel O: Vitale Farbungen am menschlicken Auge: III. Morphologie der Schleimsekration der Konjunktiva . Z Augenheilkd 1923;51:257-270. 15. Bang BG, Bang FB: Responses of upper respiratory mucosae to dehydration and infection . Ann NY Acad Sci 1963;106:625-640.Crossref 16. Florey HW: Electron microscopic observations on goblet cells of the rat colon . Q J Exp Physiol 1960;45:329-336. 17. Shearman DJC, Muir AR: Observations on the secretory cycle of goblet cells . Q J Exp Physiol 1960;45:337-342. 18. Allansmith MR, Korb DR, Greiner JV, et al: Giant papillary conjunctivitis in contact lens wearers . Am J Ophthalmol 1977;83:697-708. 19. Fowler SA, Greiner JV, Allansmith MR: Soft contact lenses from patients with giant papillary conjunctivitis . Am J Ophthalmol 1979;88:1056-1061. 20. Norn MS: Ultrastructure of mucous thread in interior conjunctival fornix . Acta Ophthalmol 1967;45:727-732.

Journal

Archives of OphthalmologyAmerican Medical Association

Published: Oct 1, 1982

References